<StimulationSet>


    <!-- the injectionSite must match a regionLabel defined in the morphology  -->
   
    <!-- one dendrite injection, sa2[:], is distributed over all the spine
	 (each spine gets total/num spines) 
	 four Glu and Ca pulses at 50 Hz, repeated 10x= 40 pulses per train -->

  <!-- This Da is single pulse, representing 4 pulse burst, burst 1 amp = 2x 1st pulse of 20 Hz
    longer duration of inject provides for the >100msegion rise time-->
  <!-- the 15 s repetition of train provides the first burst for each of 10 trains -->
<InjectionStim specieID="AIP" injectionSite="dend:submembrane">
        <onset>               2000          </onset>
        <duration>            60      </duration>
        <rate>                45         </rate>
</InjectionStim>
<InjectionStim specieID="AIP" injectionSite="sa1[0].pointA">
        <onset>               2000          </onset>
        <duration>            60      </duration>
        <rate>                25         </rate>
</InjectionStim>

<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            60              </duration>
        <rate>                6.0           </rate>
        <period>              105             </period>
        <end>                 20100          </end>
  </InjectionStim>

  <!-- These next Da are single pulse, represent 4 pulse burst; burst 2-10 amp = 1/2 amp of 1st burst -->
  <!-- the 15 s repetition of train provides bursts 2-10 for each of 10 trains -->
  <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               20095          </onset>
        <duration>            60              </duration>
        <rate>                2.5           </rate>
        <period>              95             </period>
        <end>                 20949          </end>
  </InjectionStim>

  <!-- bursts 2-10 are lower amplitude than 1st burst -->
  <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            60              </duration>
        <rate>                4.0           </rate>
        <period>              105             </period>
        <end>                 35100          </end>
        <numTrains>           9             </numTrains>
        <interTrainInterval>  14900             </interTrainInterval>    
  </InjectionStim>

  <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               35095          </onset>
        <duration>            60              </duration>
        <rate>                1.5           </rate>
        <period>              95             </period>
        <end>                 35949          </end>
        <numTrains>           9             </numTrains>
        <interTrainInterval>  14051             </interTrainInterval>    
  </InjectionStim>

<!-- basal ACh value prior to and after stim  -->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               0          </onset>
        <duration>            20000      </duration>
        <rate>                0.2         </rate>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               170000          </onset>
        <duration>            200000      </duration>
        <rate>                0.2         </rate>
</InjectionStim>
<!-- basal ACh stim paused during Da stim-->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               23200          </onset>
        <duration>            11800      </duration>
        <rate>                0.2         </rate>
        <period>              15000             </period>
        <end>                 170000          </end>
</InjectionStim>

 <!-- burst during stim specified for each Glu/Da train (below)-->
 <!-- rebound burst: develops more slowly -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               22400          </onset>
        <duration>            800            </duration>
        <rate>                0            </rate>
        <period>              900           </period>
        <end>                 23299          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  14101          </interTrainInterval>  
</InjectionStim>


   <!-- *****************TRAIN 1 *** Glu inject **********-->   
  <InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
  </InjectionStim>

<!--  Ca pulses TRAIN 1:  40 Ca pulses -->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               20000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               20000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--**********Glu *******TRAIN 2************-->
  <InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>  
  </InjectionStim>
  
<!--**********Calcium******TRAIN 2******* 80 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               35000          </onset>
        <duration>            3              </duration>
        <rate>               156            </rate>
        <period>              20             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               35000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 3***************-->
  <InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               50000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
  </InjectionStim>
  
<!--**********Calcium******TRAIN 3******* 120 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               50000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>      
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               50000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>   
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               50000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               50000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--**********Glu******TRAIN 4******************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               65000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>
  
<!--**********Calcium******TRAIN 4******* 160 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               65000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval> 
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               65000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval> 
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               65000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               65000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--*********Glu******TRAIN 5***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               80000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>  
</InjectionStim>

<!--**********Calcium******TRAIN 5******* 200 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               80000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               80000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               80000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               80000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--**********Glu******TRAIN 6******************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               95000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 6******* 240 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               95000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               95000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>   
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               95000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               95000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 7***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              110000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 7******* 280 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              110000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              110000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               110000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               110000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 8***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              125000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 8******* 320 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              125000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>      
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              125000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               125000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               125000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--*********Glu******TRAIN 9***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              140000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 9******* 360 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              140000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              140000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               140000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               140000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 10***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              155000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
      </InjectionStim>
      
<!--**********Calcium******TRAIN 10******* 320 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              155000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              155000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               155000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               155000          </onset>
        <duration>            15              </duration>
        <rate>               3.0          </rate>
        <period>              40             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


</StimulationSet>