create soma
access soma

membranecap = 1    /* specific membrane capacitance in uF cm^-2 */
membraneresist = 40000 /* specific membrane resistance in ohm cm^2 */
axialresist = 100     /* axial resistivity in ohm cm */
Vrest = -70               /* resting potential, mV */
ECA=60
Gbar=5
V05=-10
Z=10

objref gplot, vplot, vC,BOX

proc load_cell() {

// $s1 filename

  aspiny = 0
  forall delete_section()
  xopen($s1)
  access soma
	forall {
		nseg*=5
		insert pas
		insert Calcium
		gbar_Calcium=Gbar
	    v12_Calcium=V05
	    vSlope_Calcium=Z
	    tau_Calcium=3
		e_pas = Vrest
		eca= ECA
		g_pas=1/membraneresist 
		Ra=axialresist 
		cm=membranecap
	}
	soma  vC = new SEClamp(0.5)
	vC.amp1=-120
	vC.dur1=15400
	vC.amp2=0
	vC.dur2=1000

	vC.rs=1e-5
 }   




proc DoIt(){

	finitialize(Vrest)
	fcurrent()

	dt=100
	for i=0,149 fadvance()

	dt=10
	for i=0,39 fadvance()

	dt=0.1
	for i=0,99 fadvance()

	dt=0.025
	for j=0,49 for i=0,39 fadvance()

	BOX = new HBox()
	BOX.intercept(1)
	vplot = new PlotShape()
	vplot.variable("v")
	vplot.scale(-10,80)
	vplot.exec_menu("Show Diam")
	vplot.exec_menu("Shape Plot")

	gplot = new PlotShape()
	gplot.variable("gca_Calcium")
	gplot.scale(0,Gbar+0.5)
	gplot.exec_menu("Show Diam")
	gplot.exec_menu("Shape Plot")
	xpanel("Information")
	xlabel("The left graph displays the membrane potential") 
	xlabel("in pseudo color in each compartment of the neuron while")
	xlabel("the right graph displays the activation of the")
	xlabel("voltage-gated conductance also in  pseudo color.")
	xlabel("Change the value of the conductance density in ")
	xlabel("panel or cell type to observe the different decay.")
	xpanel()
	BOX.intercept(0)
	BOX.map()
} 

proc fig1a() {
  load_cell("cells/A0606.hoc")
  DoIt()
}

proc fig1b() {
  load_cell("cells/C050800E2.hoc")  
  DoIt()
}

proc fig1c() {
  load_cell("cells/C230998A-I3.hoc")
  DoIt()
}

proc fig1d() {
  load_cell("cells/j7_L4stellate.hoc") 
  DoIt()
}

xpanel("Simulate")
xvalue("Calcium conductance density", "Gbar")
xbutton("1. L5 Pyramidal Neuron","fig1a()")
xbutton("2. L4 interneuron","fig1b()")
xbutton("3. L2/3 bipolar interneuron ","fig1c()")
xbutton("4. L4 spiny stellate ","fig1d()")
xpanel()