{\rtf1\adeflang1025\ansi\ansicpg10000\uc1\adeff31507\deff0\stshfdbch31506\stshfloch31506\stshfhich31506\stshfbi31507\deflang1033\deflangfe1033\themelang1033\themelangfe1041\themelangcs0{\upr{\fonttbl{\f0\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}{\f23\fbidi \fnil\fcharset0\fprq2{\*\panose 02040503050406030204}Cambria;}
{\f208\fbidi \fnil\fcharset0\fprq2 Lucida Grande;}{\flomajor\f31500\fbidi \fnil\fcharset0\fprq2{\*\panose 020b0604020202020204}Arial;}{\fdbmajor\f31501\fbidi \fnil\fcharset78\fprq2 \'82\'6c\'82\'72 \'83\'53\'83\'56\'83\'62\'83\'4e;}
{\fhimajor\f31502\fbidi \fnil\fcharset0\fprq2{\*\panose 020f0502020204030204}Calibri;}{\fbimajor\f31503\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}
{\flominor\f31504\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}{\fdbminor\f31505\fbidi \fnil\fcharset78\fprq2 \'82\'6c\'82\'72 \'96\'be\'92\'a9;}
{\fhiminor\f31506\fbidi \fnil\fcharset0\fprq2{\*\panose 02040503050406030204}Cambria;}{\fbiminor\f31507\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}
}{\*\ud{\fonttbl{\f0\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}{\f23\fbidi \fnil\fcharset0\fprq2{\*\panose 02040503050406030204}Cambria;}{\f208\fbidi \fnil\fcharset0\fprq2 Lucida Grande;}
{\flomajor\f31500\fbidi \fnil\fcharset0\fprq2{\*\panose 020b0604020202020204}Arial;}{\fdbmajor\f31501\fbidi \fnil\fcharset78\fprq2 \u-211 _\u-205 _ \u12468 _\u12471 _\u12483 _\u12463 _;}
{\fhimajor\f31502\fbidi \fnil\fcharset0\fprq2{\*\panose 020f0502020204030204}Calibri;}{\fbimajor\f31503\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}
{\flominor\f31504\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}{\fdbminor\f31505\fbidi \fnil\fcharset78\fprq2 \u-211 _\u-205 _ \u26126 _\u26397 _;}
{\fhiminor\f31506\fbidi \fnil\fcharset0\fprq2{\*\panose 02040503050406030204}Cambria;}{\fbiminor\f31507\fbidi \fnil\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}}}}{\colortbl;\red0\green0\blue0;\red0\green0\blue255;
\red0\green255\blue255;\red0\green255\blue0;\red255\green0\blue255;\red255\green0\blue0;\red255\green255\blue0;\red255\green255\blue255;\red0\green0\blue128;\red0\green128\blue128;\red0\green128\blue0;\red128\green0\blue128;\red128\green0\blue0;
\red128\green128\blue0;\red128\green128\blue128;\red192\green192\blue192;\chyperlink\ctint255\cshade255\red0\green0\blue255;\cfollowedhyperlink\ctint255\cshade255\red128\green0\blue128;}{\*\defchp \f31506 }{\*\defpap 
\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 }\noqfpromote {\stylesheet{\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 
\f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 \snext0 Normal;}{\*\cs10 \additive \ssemihidden Default Paragraph Font;}{\*
\ts11\tsrowd\trftsWidthB3\trpaddl108\trpaddr108\trpaddfl3\trpaddft3\trpaddfb3\trpaddfr3\tblind0\tblindtype3\tscellwidthfts0\tsvertalt\tsbrdrt\tsbrdrl\tsbrdrb\tsbrdrr\tsbrdrdgl\tsbrdrdgr\tsbrdrh\tsbrdrv 
\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 \rtlch\fcs1 \af31507\afs20\alang1033 \ltrch\fcs0 \f31506\fs20\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 \snext11 \ssemihidden Normal Table;}{
\s15\ql \li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin720\itap0\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 
\sbasedon0 \snext15 \styrsid1070915 List Paragraph;}{\*\cs16 \additive \rtlch\fcs1 \af0 \ltrch\fcs0 \ul\cf17 \sbasedon10 \ssemihidden \styrsid7606648 Hyperlink;}{\*\cs17 \additive \rtlch\fcs1 \af0 \ltrch\fcs0 \ul\cf18 
\sbasedon10 \ssemihidden \styrsid4868440 FollowedHyperlink;}{\s18\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 \rtlch\fcs1 \af31507\afs18\alang1033 \ltrch\fcs0 
\f208\fs18\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 \sbasedon0 \snext18 \slink19 \ssemihidden \styrsid12129 Balloon Text;}{\*\cs19 \additive \rtlch\fcs1 \af0 \ltrch\fcs0 \f208\fs18 \sbasedon10 \slink18 \slocked \ssemihidden \styrsid12129 
Balloon Text Char;}{\*\cs20 \additive \rtlch\fcs1 \af0 \ltrch\fcs0 \fs18 \sbasedon10 \ssemihidden \styrsid3694277 annotation reference;}{\s21\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 \rtlch\fcs1 
\af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 \sbasedon0 \snext21 \slink22 \ssemihidden \styrsid3694277 annotation text;}{\*\cs22 \additive \rtlch\fcs1 \af0 \ltrch\fcs0 \fs24 
\sbasedon10 \slink21 \slocked \ssemihidden \styrsid3694277 Comment Text Char;}{\s23\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 \rtlch\fcs1 \ab\af31507\afs20\alang1033 \ltrch\fcs0 
\b\f31506\fs20\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 \sbasedon21 \snext21 \slink24 \ssemihidden \styrsid3694277 annotation subject;}{\*\cs24 \additive \rtlch\fcs1 \ab\af0 \ltrch\fcs0 \b\fs24 
\sbasedon22 \slink23 \slocked \ssemihidden \styrsid3694277 Comment Subject Char;}}{\*\listtable{\list\listtemplateid1292264956\listhybrid{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0{\leveltext
\leveltemplateid67698703\'02\'00.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fbias0 \fi-360\li720\lin720 }{\listlevel\levelnfc4\levelnfcn4\leveljc0\leveljcn0\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698713\'02\'01.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-360\li1440\lin1440 }{\listlevel\levelnfc2\levelnfcn2\leveljc2\leveljcn2\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698715\'02\'02.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-180\li2160\lin2160 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698703\'02\'03.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-360\li2880\lin2880 }{\listlevel\levelnfc4\levelnfcn4\leveljc0\leveljcn0\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698713\'02\'04.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-360\li3600\lin3600 }{\listlevel\levelnfc2\levelnfcn2\leveljc2\leveljcn2\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698715\'02\'05.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-180\li4320\lin4320 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698703\'02\'06.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-360\li5040\lin5040 }{\listlevel\levelnfc4\levelnfcn4\leveljc0\leveljcn0\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698713\'02\'07.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-360\li5760\lin5760 }{\listlevel\levelnfc2\levelnfcn2\leveljc2\leveljcn2\levelfollow0\levelstartat1\lvltentative\levelspace0\levelindent0{\leveltext
\leveltemplateid67698715\'02\'08.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \fi-180\li6480\lin6480 }{\listname ;}\listid1595212809}}{\*\listoverridetable{\listoverride\listid1595212809\listoverridecount0\ls1}}{\*\rsidtbl \rsid12129\rsid474793
\rsid590647\rsid675284\rsid1070915\rsid1407234\rsid1988182\rsid3694277\rsid3757429\rsid3766145\rsid4004595\rsid4211956\rsid4605507\rsid4653395\rsid4868440\rsid4868963\rsid5059091\rsid5251163\rsid5468400\rsid5530802\rsid5706046\rsid5726795\rsid6176863
\rsid6320231\rsid6890987\rsid6910273\rsid7606648\rsid7756216\rsid8533820\rsid8869077\rsid8922863\rsid9137215\rsid9448945\rsid9718770\rsid10253584\rsid11172777\rsid11275082\rsid11490050\rsid11954081\rsid13641415\rsid14039451\rsid14558486\rsid14771632
\rsid15095093\rsid15486597\rsid15824208\rsid16207401\rsid16392562\rsid16517658}{\mmathPr\mmathFont34\mbrkBin0\mbrkBinSub0\msmallFrac1\mdispDef1\mlMargin0\mrMargin0\mdefJc1\mwrapRight1\mintLim0\mnaryLim0}{\info{\author Anwar Haroon}{\operator Anwar Haroon}
{\creatim\yr2011\mo3\dy30\hr12\min17}{\revtim\yr2011\mo3\dy30\hr12\min17}{\version2}{\edmins0}{\nofpages3}{\nofwords757}{\nofchars4319}{\*\company OIST}{\nofcharsws5304}{\vern33041}{\*\saveprevpict}}{\*\xmlnstbl {\xmlns1 http://schemas.microsoft.com/offic
e/word/2003/wordml}}\paperw11900\paperh16840\margl1800\margr1800\margt1440\margb1440\gutter0\ltrsect 
\ftnbj\aenddoc\trackmoves0\trackformatting1\donotembedsysfont0\relyonvml0\donotembedlingdata0\grfdocevents0\validatexml0\showplaceholdtext0\ignoremixedcontent0\saveinvalidxml0\showxmlerrors0\noxlattoyen
\expshrtn\noultrlspc\dntblnsbdb\nospaceforul\formshade\horzdoc\dghspace180\dgvspace180\dghorigin1701\dgvorigin1984\dghshow0\dgvshow0
\jexpand\viewkind1\viewscale200\pgbrdrhead\pgbrdrfoot\splytwnine\ftnlytwnine\htmautsp\nolnhtadjtbl\useltbaln\alntblind\lytcalctblwd\lyttblrtgr\lnbrkrule\nobrkwrptbl\snaptogridincell\allowfieldendsel\wrppunct
\asianbrkrule\nojkernpunct\rsidroot13641415\newtblstyruls\nogrowautofit\usenormstyforlist\noindnmbrts\felnbrelev\nocxsptable\indrlsweleven\afelev\utinl\hwelev\notvatxbx \nouicompat \fet0{\*\wgrffmtfilter 013f}\nofeaturethrottle1\ilfomacatclnup0
\stylesortmethod0\ltrpar \sectd \ltrsect\linex0\endnhere\sectdefaultcl\sectrsid1070915\sftnbj {\*\pnseclvl1\pnucrm\pnstart1\pnindent720\pnhang {\pntxta .}}{\*\pnseclvl2\pnucltr\pnstart1\pnindent720\pnhang {\pntxta .}}{\*\pnseclvl3
\pndec\pnstart1\pnindent720\pnhang {\pntxta .}}{\*\pnseclvl4\pnlcltr\pnstart1\pnindent720\pnhang {\pntxta )}}{\*\pnseclvl5\pndec\pnstart1\pnindent720\pnhang {\pntxtb (}{\pntxta )}}{\*\pnseclvl6\pnlcltr\pnstart1\pnindent720\pnhang {\pntxtb (}{\pntxta )}}
{\*\pnseclvl7\pnlcrm\pnstart1\pnindent720\pnhang {\pntxtb (}{\pntxta )}}{\*\pnseclvl8\pnlcltr\pnstart1\pnindent720\pnhang {\pntxtb (}{\pntxta )}}{\*\pnseclvl9\pnlcrm\pnstart1\pnindent720\pnhang {\pntxtb (}{\pntxta )}}\pard\plain \ltrpar
\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 \af31507 
\ltrch\fcs0 \b\f0\ul\insrsid13641415\charrsid15095093 Calcium buffering models explanatory note:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid13641415\charrsid15095093 Model Published in:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 
\par Anwa}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 r H, Hong S, De Schutter E (201}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4211956 0}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 ) Controlling Ca}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\super\insrsid13641415\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 -activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid13641415\charrsid15095093 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid13641415\charrsid15095093  channels with models of Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid13641415\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093  buffering in Purkinje cell.}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648\charrsid7606648 Cerebellum}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid4868440  *}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648  }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 
\par 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid4868440 * }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648\charrsid4868440 Available as Open Access
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid7606648 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid7606648\charrsid4868440 PubMed link}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid7606648 : }{\field\fldedit{\*\fldinst {\rtlch\fcs1 \af31507 \ltrch\fcs0 \insrsid9448945 HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed/20981513"}}{\fldrslt {\rtlch\fcs1 \af31507 \ltrch\fcs0 \cs16\f0\ul\cf17\insrsid7606648\charrsid12670074 
http://www.ncbi.nlm.nih.gov/pubmed/20981513}}}\sectd \ltrsect\linex0\endnhere\sectdefaultcl\sectrsid1070915\sftnbj {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987 
\par Email contact: }{\field{\*\fldinst {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987  HYPERLINK "mailto:anwar@oist.jp" }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987\charrsid12670074 {\*\datafield 
00d0c9ea79f9bace118c8200aa004ba90b02000000170000000e00000061006e0077006100720040006f006900730074002e006a0070000000e0c9ea79f9bace118c8200aa004ba90b2a0000006d00610069006c0074006f003a0061006e0077006100720040006f006900730074002e006a0070000000}}}{\fldrslt {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \cs16\f0\ul\cf17\insrsid6890987\charrsid12670074 anwar@oist.jp}}}\sectd \ltrsect\linex0\endnhere\sectdefaultcl\sectrsid1070915\sftnbj {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6890987 
\par 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid13641415\charrsid15095093 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid4004595\charrsid15095093 Model description:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4004595\charrsid15095093 
\par In this work, we }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 compare the dynamics of different buffering models during generation of a dendritic Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+
}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  spike in a single compartment model of a Purkinje cell dendrite.}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4004595\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid10253584\charrsid15095093  The Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  buffering models used are 1) a single Ca}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  pool, 2) two Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid10253584\charrsid15095093 2+}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  pools respectively }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14558486\charrsid15095093 f}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 
or the fast and slow transients, 3) a detailed calcium model with buffers, pump}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562  (Schmidt et al., 2003)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5726795 ,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid10253584\charrsid15095093  and diffusion and 4) a calcium model with buffers, pump and diffusion compensation. The parameters of }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 single pool and double pool are}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093  tuned, using Neurofitter}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231\charrsid15095093 (Van Geit }{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231\charrsid15095093  2007)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 , to approximate the behavior of detailed calcium dynami}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5706046\charrsid15095093 cs over range of 0.5 \u181\'b5M to 8 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid10253584\charrsid15095093 M}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid14558486\charrsid15095093  of intracellular calcium}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 . The diffusion compensation is}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14558486\charrsid15095093  modele}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 d using a buffer}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid12129 -}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 like mechanism}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid8869077  called }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 DCM. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 To use DCM robustly for different diameter compartments, its}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093  parameters}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11172777\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 are}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid8533820\charrsid15095093  estimated}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231 ,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093  using Neurofitter}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid14039451\charrsid15095093  (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14039451\charrsid15095093  2007)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6320231 ,}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11172777\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 as a fu}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 nction of com}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid5706046\charrsid15095093 partment diameter  (0.8 \u181\'b5m-20 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 m)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093 .}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7756216\charrsid15095093 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8533820\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14558486\charrsid15095093 
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\f0\insrsid1070915\charrsid15095093 Scripts Explanation:
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
\par Go to the folder containing the model scripts. Run nrnivmodl. Then type nrngui CaBufferingModels.hoc
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\outlinelevel0\adjustright\rin0\lin0\itap0\pararsid12129 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 A panel with six}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1070915\charrsid15095093  buttons will appear on the screen. 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid15824208\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 1.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid1070915\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid15824208\charrsid15095093 Detailed Calcium }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093 dynamics model}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
: Click on this button to see the behavior of }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093 detailed c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16207401\charrsid15095093 
alcium dynamics model. It will display nine plots arranged in three rows and three columns. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093 The plot on the top left corner shows }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid9718770\charrsid15095093 c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 alcium transients}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093  with }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid5706046\charrsid15095093 peak amplitude of 0.5 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11490050 M, 1 }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11490050\charrsid15095093 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid5706046\charrsid15095093 M and 2 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 M}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093 
. These transients were generated using a voltage step command (as shown in Figure 1a}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid8922863 {\*\bkmkstart _GoBack}{\*\bkmkend _GoBack}2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093 ) with different maximal condu}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093 
ctance values of P-type }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868440 Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid4868440\charrsid4868440 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid11275082\charrsid15095093  channel. }{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 All the other plots show corresponding behavior of buffers and pump current.}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid590647\charrsid15095093  These plots are same as Figur}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid14771632\charrsid15095093 e 2 in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid590647\charrsid15095093 .}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4004595\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 
\par }\pard \ltrpar\s15\ql \li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin720\itap0\pararsid15824208\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093  
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1070915\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 2.\tab}}\pard \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid15824208\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093 
Calcium transients using different buffering models}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 : Click on this button to see the calcium trans}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5706046\charrsid15095093 
ients with peak amplitude of 2 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093 M, generated using single pool, double pool, detailed calcium dynamics model and DCM. The parameters of single pool}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid590647\charrsid15095093 , double pool and DCM were fitted using Neurofitter}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14039451\charrsid15095093  (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14039451\charrsid15095093  2007)}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid9718770\charrsid15095093  as described in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid590647\charrsid15095093 .}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid15824208\charrsid15095093  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid5059091\charrsid15095093 This }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6176863\charrsid15095093 plot is si}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5530802 m}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963 
ilar to Figure 3c and Figure 6 }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963\charrsid15095093 in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963 Anwar et al., 2010 }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5530802 except that the Ca}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid5530802\charrsid5468400 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5530802  transient in these scri}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid5468400 pts are shown for diameter 4 }{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid5468400\charrsid15095093 \u181\'b5}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid4868963 m}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid6176863\charrsid15095093 . }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid15824208\charrsid15095093 
\par }\pard \ltrpar\s15\ql \li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin720\itap0\pararsid15824208\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1070915\charrsid15095093 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1070915\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 3.\tab}}\pard \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid5059091\contextualspace {\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \i\f0\insrsid1988182 single pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1070915\charrsid15095093  model}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid590647\charrsid15095093 : }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid4605507\charrsid15095093 Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid14771632\charrsid15095093 generated using single pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
 model. The maximal conductance values for P-type and T-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
 channels were tuned using Neurofitter (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 
Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 . Note that the parameter values used for single pool in this plot are different than those use
d in plot showing calcium transients. The parameter values of single pool used to generate calcium spikes in this plot are obtained using experimental trace (shown in Figure 1b in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 ) as a voltage command. This plot }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  in Figure 7}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658 d}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1988182\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 4.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid5059091\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182 double pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093  model}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 : Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 generated using double}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 
 pool}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  model. The maximal conductance values for P-type and T-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
 channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels were tuned using Neurofitter (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 
. Note that the parameter values used for double pool in this plot are different than those used in plot showing calcium transients. The parameter values 
of double pool used to generate calcium spikes in this plot are obtained using experimental trace (shown in Figure 1b in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 ) as a voltage command. This plot }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658  in Figure 7d}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1988182\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 5.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid5059091\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182 detailed}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093  model}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 : Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 generated using detailed model. The maximal conductance values for P-type and T-type Ca}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels were tuned using Neurofitter (Van Geit }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 . This plot }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658  in Figure 7c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af31507 \ltrch\fcs0 \i\insrsid1988182\charrsid15095093 \hich\af0\dbch\af31506\loch\f0 6.\tab}}\pard\plain \ltrpar
\s15\ql \fi-360\li720\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\ls1\adjustright\rin0\lin720\itap0\pararsid1988182\contextualspace \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {
\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182\charrsid15095093 Calcium spikes using }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid1988182 DCM}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid15095093 
: Click on this button to see the calcium spikes }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 generated using DCM. The maximal conductance values for P-type and T-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 
2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels and BK-type and SK-type Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  activated K}{\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\super\insrsid1988182\charrsid6910273 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  channels were tuned using Neurofitter (Van Geit }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 et al.,}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid1988182  2007) and are presented in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 . This plot }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid7606648 exists}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16517658  in Figure 7c}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182  in }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 Anwar et al., }{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182 .
\par }\pard\plain \ltrpar\ql \li360\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin360\itap0\pararsid1988182 \rtlch\fcs1 \af31507\afs24\alang1033 \ltrch\fcs0 \f31506\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\rtlch\fcs1 
\af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid1988182 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid1988182\charrsid1988182 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\insrsid9137215\charrsid9137215 References}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\insrsid1070915 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \b\insrsid16392562\charrsid16392562 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid15095093 Anwa}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 r H, Hong S, De Schutter E (}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid8922863 2010}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid16392562\charrsid15095093 ) Controlling Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid16392562\charrsid15095093 2+}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid15095093 -activated K}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\super\insrsid16392562\charrsid15095093 +}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid15095093  channels with models of Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid16392562\charrsid15095093 2+}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid16392562\charrsid15095093  buffering in Purkinje cell.}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562  }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid16392562\charrsid7606648 Cerebellum}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\f0\insrsid16392562 , in print.
\par 
\par }\pard \ltrpar\ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid16392562 {\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562 
van Geit W, Achard P, de Schutter E (2007) Neurofitter: a parameter tuning package for a wide range of electrophysiological neuron models. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid474793\charrsid474793 Front}{\rtlch\fcs1 \af31507 \ltrch\fcs0 
\i\f0\insrsid9137215\charrsid9137215  Neuroinform}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562  1:1
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562 
\par }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562 Schmidt H, Stiefel K, Racay P, Schwaller B, Eilers J (2003) Mutational analysis of dendritic Ca}{\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\super\insrsid9137215\charrsid9137215 2+}{
\rtlch\fcs1 \af31507 \ltrch\fcs0 \f0\insrsid16392562\charrsid16392562  kinetics in rodent Purkinje cells: role of parvalbumin and calbindin D28k. }{\rtlch\fcs1 \af31507 \ltrch\fcs0 \i\f0\insrsid9137215\charrsid9137215 J Physiol}{\rtlch\fcs1 \af31507 
\ltrch\fcs0 \f0\insrsid16392562\charrsid16392562  551:13-32
\par }{\*\themedata 504b0304140006000800000021001b898e1bfe0000001c020000130000005b436f6e74656e745f54797065735d2e786d6cac91cf6ac3300cc6ef83bd83f17524
4e77186324e961ed6efb73e81e40384a6296c8c6564bfbf653d216c62885c12e0659d2f7fd2495cbfd38a81dc6e43c557a91175a2159df38ea2afdb979c91eb5
4a0cd4c0e0092b7dc0a497f5ed4db939044c4aba2955ba670e4fc624dbe30829f7014932ad8f23b084b13301ec177468ee8be2c1584f8cc4194f1aba2e57d8c2
7660b5decbf79144dab57a3ed64d5695861006678105d44c595397ef021e5d83ea0322bfc128558605017fbe8bfcbad68e9a5fc0996f5b67b1f1763b0a663e4b
defdc5f31548868dffe37c123b035c5856c4215dd9d685094fe7c8a573de68ea5d48670733dfb6fe060000ffff0300504b030414000600080000002100a5d6a7
e7c0000000360100000b0000005f72656c732f2e72656c73848fcf6ac3300c87ef85bd83d17d51d2c31825762fa590432fa37d00e1287f68221bdb1bebdb4fc7
060abb0884a4eff7a93dfeae8bf9e194e720169aaa06c3e2433fcb68e1763dbf7f82c985a4a725085b787086a37bdbb55fbc50d1a33ccd311ba548b63095120f
88d94fbc52ae4264d1c910d24a45db3462247fa791715fd71f989e19e0364cd3f51652d73760ae8fa8c9ffb3c330cc9e4fc17faf2ce545046e37944c69e462a1
a82fe353bd90a865aad41ed0b5b8f9d6fd010000ffff0300504b0304140006000800000021006b799616830000008a0000001c0000007468656d652f7468656d
652f7468656d654d616e616765722e786d6c0ccc4d0ac3201040e17da17790d93763bb284562b2cbaebbf600439c1a41c7a0d29fdbd7e5e38337cedf14d59b4b
0d592c9c070d8a65cd2e88b7f07c2ca71ba8da481cc52c6ce1c715e6e97818c9b48d13df49c873517d23d59085adb5dd20d6b52bd521ef2cdd5eb9246a3d8b47
57e8d3f729e245eb2b260a0238fd010000ffff0300504b0304140006000800000021009d5c8bbe10070000871d0000160000007468656d652f7468656d652f74
68656d65312e786d6cec594f6f1b4514bf23f11d467b6f13274e9a4475aad8b109b469a3d82dea71bc3bf64e33bbb39a1927f10db547242444411ca8c48d0302
2ab51297f269024550a47e05deccecae77e271e39400153487d63bfb7b6fdefbbd3ff367af5e3b4e183a2442529e3682dae5c5009134e4114d878de076af7369
2d4052e134c28ca7a4118c890cae6dbefbce55bca162921004f2a9dcc08d20562adb585890210c63799967248577032e12ace0510c1722818f406fc216961617
5717124cd300a53801b5b706031a12d4d32a83cd42799bc163aaa41e0899e86ad5c49130d8e8a0a611722c5b4ca043cc1a01cc13f1a31e39560162582a78d108
16cd5fb0b07975016fe4424ccd90adc875cc5f2e970b44074b664e31ec9793d63af5f52bdba57e03606a1ad76eb75bed5aa9cf00701882a7d696aace7a67add6
2c745640f6e7b4eed6e2ca62ddc557f42f4fd9bcde6c3657d6735bac5203b23feb53f8b5c5d5fad6928337208b5f99c2d79b5badd6aa8337208b5f9dc277aeac
afd65dbc01c58ca60753681dd04e27d75e42069ced78e16b005f5bcce113146443995d7a8a014fd5ac5c4bf03d2e3a00d04086154d911a67648043c8e21666b4
2fa89e006f105c796387423935a4e742321434538de0830c43454cf4bd7cf6ddcb674fd0c9fda727f77f3c79f0e0e4fe0f569123b583d36155eac5379ffef1e8
23f4fb93af5f3cfcdc8f9755fc2fdf7ffcf34f9ff981503e13739e7ff1f8d7a78f9f7ff9c96fdf3ef4c0b704ee57e13d9a10896e9223b4cf1370ccb0e25a4efa
e27c12bd18d3aac4563a9438c57a168ffeb68a1df4cd3166d8836b1297c13b02da870ff8dee89e637037162395c7dbf1ec7a9c38c05dce59930b2f0bd7f55c15
9a7ba374e89f5c8caab87d8c0f7d73b770eac4b73dcaa06f529fca564c1c33f7184e151e929428a4dff103423c7cdda5d4e1759786824b3e50e82e454d4cbd94
f468dfc9a689d00e4d202e639f81106f879bdd3ba8c999cfeb6d72e822a12a30f318df23cca1f13d3c5238f1a9ece1845509bf8155ec33b23b166115d7960a22
3d248ca37644a4f4c9dc12e06f25e8d7a175f8c3becbc6898b148a1ef874dec09c5791dbfca015e324f361bb348dabd8f7e501a428467b5cf9e0bbdcad10fd0c
71c0e9cc70dfa1c409f7d9dde0361d3a264d1244bf19091d4b68d54e074e68faaa769c4037ceddb9b8760c0df0f9578f3c99f5a636e22d20c157093ba7daef2c
dce9a6dbe222a26f7ecfddc6a3748f409a4f2f3c6f5beedb961bfce75beeac7a9eb7d14e7a2bb45dbdbdb19b62b3454e66ee900794b1ae1a3372439a4db28475
22eac0a09633a743529e98b2187ee67dddc10d0536324870f521557137c6196cb06b81563294b9eaa144199770b033c35edd1a0f9b74658f852bfac060fb81c4
6a97477678590f17e782528d596d86e6f0594cb4ac15cc3bd9f2955c29b8fd3a93d5b45173cf5633a69956e7cc56ba0c319c760d064b3661038260db022cafc2
f95c4f0d0713cc48a479b76b6f11161385bf2744b9d7d6911847c486c819aeb05933b12b52c85c10404a7942773e364bd680b4b38d3069313b7fe624b9503021
5997dda96a6269b5b6588a8e1ac1facad24a80429c3582011c49e1679241d0a4deb26136847b9d50099bb567d6a229d289c7ebfeacaac12dc38c8271ca381352
6d6319db189a5779a858aa67b2f62fadd475b25d8c0336515fc38ae53548917fcd0a08b51b5a3218905055835d19d1dcd9c7bc13f29122a21b4747a8cf46621f
43f88153ed4f4425dc2c9882d60f700da6d936afdcde9a779aeae593c1d971ccb218e7dd525fa3141567e1a6de4a1bcc53c53cf0cd6bbb71eefcaee88abf2857
aa69fc3f73452f0770d05f8e740442b8851518e97a6d045ca8984317ca621a7604acfba67740b6c0552abc06f2e12ed8fc2fc8a1fedfd69cd561ca1ace6b6a9f
0e91a0b09ca85810b2076dc964df19ca6af9d26355b25c91c9a88ab932b366f7c921613ddd0357750f0e500ca96eba49de060cee74feb9cf7905f5877a8f52ad
37a787944ba7ad817f7ae3628b199c3ab597d0f95bf05f9ae859fdacbc112fd6c8aa23fac56497542faac259fcd6d7f3a95ed3847916e0ca5a6b3bd694c74b2b
857110c5698f61b0dccf64705d83f43fb0fe511132fb61412fa83dbe0fbd15c17702cb1f82acbea4bb1a64906e90f6571ff63d76d026935665a9cd773e9ab562
b1bee08d6a39ef29b2b565f3c4fb9c64979b28773aa7162f92ec9c61876b3b36936a88ece91285a141710e3181315fa4aa1f8d78ff1e047a1baee747cc7e4692
193c993ac8f684c9ae3e8fc6f94f26ed826bb34e9f613492a5fb648068745c9c3f4a266c09d94f19c516d9a0b5984eb45270d97768700573bc16b5ab6529bc74
b67029616686965d0a9b1b329f02f89095376e7db403bc6db2d66b5d5c05532cfd2b94cd61bc9f32efc9675ecaec41f195817a0dcad4f1ab29cb9902f2a6130f
3e450a0c4793aee9bfb0e8d84c3729bbf927000000ffff0300504b0304140006000800000021000dd1909fb60000001b010000270000007468656d652f746865
6d652f5f72656c732f7468656d654d616e616765722e786d6c2e72656c73848f4d0ac2301484f78277086f6fd3ba109126dd88d0add40384e4350d363f2451ec
ed0dae2c082e8761be9969bb979dc9136332de3168aa1a083ae995719ac16db8ec8e4052164e89d93b64b060828e6f37ed1567914b284d262452282e3198720e
274a939cd08a54f980ae38a38f56e422a3a641c8bbd048f7757da0f19b017cc524bd62107bd5001996509affb3fd381a89672f1f165dfe514173d9850528a2c6
cce0239baa4c04ca5bbabac4df000000ffff0300504b01022d00140006000800000021001b898e1bfe0000001c02000013000000000000000000000000000000
00005b436f6e74656e745f54797065735d2e786d6c504b01022d0014000600080000002100a5d6a7e7c0000000360100000b000000000000000000000000002f
0100005f72656c732f2e72656c73504b01022d00140006000800000021006b799616830000008a0000001c00000000000000000000000000180200007468656d
652f7468656d652f7468656d654d616e616765722e786d6c504b01022d00140006000800000021009d5c8bbe10070000871d0000160000000000000000000000
0000d50200007468656d652f7468656d652f7468656d65312e786d6c504b01022d00140006000800000021000dd1909fb60000001b0100002700000000000000
000000000000190a00007468656d652f7468656d652f5f72656c732f7468656d654d616e616765722e786d6c2e72656c73504b050600000000050005005d010000140b00000000}
{\*\colorschememapping 3c3f786d6c2076657273696f6e3d22312e302220656e636f64696e673d225554462d3822207374616e64616c6f6e653d22796573223f3e0d0a3c613a636c724d
617020786d6c6e733a613d22687474703a2f2f736368656d61732e6f70656e786d6c666f726d6174732e6f72672f64726177696e676d6c2f323030362f6d6169
6e22206267313d226c743122207478313d22646b3122206267323d226c743222207478323d22646b322220616363656e74313d22616363656e74312220616363
656e74323d22616363656e74322220616363656e74333d22616363656e74332220616363656e74343d22616363656e74342220616363656e74353d22616363656e74352220616363656e74363d22616363656e74362220686c696e6b3d22686c696e6b2220666f6c486c696e6b3d22666f6c486c696e6b222f3e}
{\*\latentstyles\lsdstimax276\lsdlockeddef0\lsdsemihiddendef1\lsdunhideuseddef1\lsdqformatdef0\lsdprioritydef99{\lsdlockedexcept \lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority0 \lsdlocked0 Normal;
\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority9 \lsdlocked0 heading 1;\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 2;\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 3;\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 4;
\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 5;\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 6;\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 7;\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 8;\lsdqformat1 \lsdpriority9 \lsdlocked0 heading 9;
\lsdpriority39 \lsdlocked0 toc 1;\lsdpriority39 \lsdlocked0 toc 2;\lsdpriority39 \lsdlocked0 toc 3;\lsdpriority39 \lsdlocked0 toc 4;\lsdpriority39 \lsdlocked0 toc 5;\lsdpriority39 \lsdlocked0 toc 6;\lsdpriority39 \lsdlocked0 toc 7;
\lsdpriority39 \lsdlocked0 toc 8;\lsdpriority39 \lsdlocked0 toc 9;\lsdqformat1 \lsdpriority35 \lsdlocked0 caption;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority10 \lsdlocked0 Title;\lsdpriority1 \lsdlocked0 Default Paragraph Font;
\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority11 \lsdlocked0 Subtitle;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority22 \lsdlocked0 Strong;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority20 \lsdlocked0 Emphasis;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority59 \lsdlocked0 Table Grid;\lsdunhideused0 \lsdlocked0 Placeholder Text;\lsdunhideused0 \lsdqformat1 \lsdpriority1 \lsdlocked0 No Spacing;\lsdsemihidden0 \lsdunhideused0 \lsdpriority60 \lsdlocked0 Light Shading;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority61 \lsdlocked0 Light List;\lsdsemihidden0 \lsdunhideused0 \lsdpriority62 \lsdlocked0 Light Grid;\lsdsemihidden0 \lsdunhideused0 \lsdpriority63 \lsdlocked0 Medium Shading 1;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority64 \lsdlocked0 Medium Shading 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority65 \lsdlocked0 Medium List 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority66 \lsdlocked0 Medium List 2;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority67 \lsdlocked0 Medium Grid 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority68 \lsdlocked0 Medium Grid 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority69 \lsdlocked0 Medium Grid 3;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority70 \lsdlocked0 Dark List;\lsdsemihidden0 \lsdunhideused0 \lsdpriority71 \lsdlocked0 Colorful Shading;\lsdsemihidden0 \lsdunhideused0 \lsdpriority72 \lsdlocked0 Colorful List;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority73 \lsdlocked0 Colorful Grid;\lsdsemihidden0 \lsdunhideused0 \lsdpriority60 \lsdlocked0 Light Shading Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority61 \lsdlocked0 Light List Accent 1;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority62 \lsdlocked0 Light Grid Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority63 \lsdlocked0 Medium Shading 1 Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority64 \lsdlocked0 Medium Shading 2 Accent 1;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority65 \lsdlocked0 Medium List 1 Accent 1;\lsdunhideused0 \lsdlocked0 Revision;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority34 \lsdlocked0 List Paragraph;
\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority29 \lsdlocked0 Quote;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority30 \lsdlocked0 Intense Quote;\lsdsemihidden0 \lsdunhideused0 \lsdpriority66 \lsdlocked0 Medium List 2 Accent 1;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority67 \lsdlocked0 Medium Grid 1 Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority68 \lsdlocked0 Medium Grid 2 Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority69 \lsdlocked0 Medium Grid 3 Accent 1;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority70 \lsdlocked0 Dark List Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority71 \lsdlocked0 Colorful Shading Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority72 \lsdlocked0 Colorful List Accent 1;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority73 \lsdlocked0 Colorful Grid Accent 1;\lsdsemihidden0 \lsdunhideused0 \lsdpriority60 \lsdlocked0 Light Shading Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority61 \lsdlocked0 Light List Accent 2;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority62 \lsdlocked0 Light Grid Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority63 \lsdlocked0 Medium Shading 1 Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority64 \lsdlocked0 Medium Shading 2 Accent 2;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority65 \lsdlocked0 Medium List 1 Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority66 \lsdlocked0 Medium List 2 Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority67 \lsdlocked0 Medium Grid 1 Accent 2;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority68 \lsdlocked0 Medium Grid 2 Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority69 \lsdlocked0 Medium Grid 3 Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority70 \lsdlocked0 Dark List Accent 2;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority71 \lsdlocked0 Colorful Shading Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority72 \lsdlocked0 Colorful List Accent 2;\lsdsemihidden0 \lsdunhideused0 \lsdpriority73 \lsdlocked0 Colorful Grid Accent 2;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority60 \lsdlocked0 Light Shading Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority61 \lsdlocked0 Light List Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority62 \lsdlocked0 Light Grid Accent 3;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority63 \lsdlocked0 Medium Shading 1 Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority64 \lsdlocked0 Medium Shading 2 Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority65 \lsdlocked0 Medium List 1 Accent 3;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority66 \lsdlocked0 Medium List 2 Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority67 \lsdlocked0 Medium Grid 1 Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority68 \lsdlocked0 Medium Grid 2 Accent 3;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority69 \lsdlocked0 Medium Grid 3 Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority70 \lsdlocked0 Dark List Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority71 \lsdlocked0 Colorful Shading Accent 3;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority72 \lsdlocked0 Colorful List Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority73 \lsdlocked0 Colorful Grid Accent 3;\lsdsemihidden0 \lsdunhideused0 \lsdpriority60 \lsdlocked0 Light Shading Accent 4;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority61 \lsdlocked0 Light List Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority62 \lsdlocked0 Light Grid Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority63 \lsdlocked0 Medium Shading 1 Accent 4;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority64 \lsdlocked0 Medium Shading 2 Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority65 \lsdlocked0 Medium List 1 Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority66 \lsdlocked0 Medium List 2 Accent 4;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority67 \lsdlocked0 Medium Grid 1 Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority68 \lsdlocked0 Medium Grid 2 Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority69 \lsdlocked0 Medium Grid 3 Accent 4;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority70 \lsdlocked0 Dark List Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority71 \lsdlocked0 Colorful Shading Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority72 \lsdlocked0 Colorful List Accent 4;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority73 \lsdlocked0 Colorful Grid Accent 4;\lsdsemihidden0 \lsdunhideused0 \lsdpriority60 \lsdlocked0 Light Shading Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority61 \lsdlocked0 Light List Accent 5;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority62 \lsdlocked0 Light Grid Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority63 \lsdlocked0 Medium Shading 1 Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority64 \lsdlocked0 Medium Shading 2 Accent 5;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority65 \lsdlocked0 Medium List 1 Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority66 \lsdlocked0 Medium List 2 Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority67 \lsdlocked0 Medium Grid 1 Accent 5;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority68 \lsdlocked0 Medium Grid 2 Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority69 \lsdlocked0 Medium Grid 3 Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority70 \lsdlocked0 Dark List Accent 5;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority71 \lsdlocked0 Colorful Shading Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority72 \lsdlocked0 Colorful List Accent 5;\lsdsemihidden0 \lsdunhideused0 \lsdpriority73 \lsdlocked0 Colorful Grid Accent 5;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority60 \lsdlocked0 Light Shading Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority61 \lsdlocked0 Light List Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority62 \lsdlocked0 Light Grid Accent 6;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority63 \lsdlocked0 Medium Shading 1 Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority64 \lsdlocked0 Medium Shading 2 Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority65 \lsdlocked0 Medium List 1 Accent 6;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority66 \lsdlocked0 Medium List 2 Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority67 \lsdlocked0 Medium Grid 1 Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority68 \lsdlocked0 Medium Grid 2 Accent 6;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority69 \lsdlocked0 Medium Grid 3 Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority70 \lsdlocked0 Dark List Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority71 \lsdlocked0 Colorful Shading Accent 6;
\lsdsemihidden0 \lsdunhideused0 \lsdpriority72 \lsdlocked0 Colorful List Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdpriority73 \lsdlocked0 Colorful Grid Accent 6;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority19 \lsdlocked0 Subtle Emphasis;
\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority21 \lsdlocked0 Intense Emphasis;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority31 \lsdlocked0 Subtle Reference;
\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority32 \lsdlocked0 Intense Reference;\lsdsemihidden0 \lsdunhideused0 \lsdqformat1 \lsdpriority33 \lsdlocked0 Book Title;\lsdpriority37 \lsdlocked0 Bibliography;
\lsdqformat1 \lsdpriority39 \lsdlocked0 TOC Heading;}}{\*\datastore 010001000200000010000000584d4c2e5341585265616465722e3500000000000000000000060000
d0cf11e0a1b11ae1000000000000000000000000000000003e000300feff090006000000000000000000000001000000010000000000000000100000feffffff00000000feffffff0000000000000000ffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
ffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
ffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
ffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
fffffffffffffffffdfffffffeffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
ffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
ffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
ffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffffff
ffffffffffffffffffffffffffffffff5200000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000500ffffffffffffffffffffffffec69d9888b8b3d4c859eaf6cd158be0f00000000000000000000000079e7
080589eecb01feffffff00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000ffffffffffffffffffffffff00000000000000000000000000000000000000000000000000000000
00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000ffffffffffffffffffffffff0000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000ffffffffffffffffffffffff000000000000000000000000000000000000000000000000
0000000000000000000000000000000000000000000000000100010000000000}}