load_file("nrngui.hoc")
load_file("morphology_mechanisms_CaTransients.hoc")
xopen("CaTransientsDisplay.ses")
celsius = 34
Dt = 0.02
steps_per_ms = 1/Dt
tstop = 5000
v_init = -90
objref pulse1, pulse2, pulse3, pulse4
objref InjectMatrix
objref InjectFile, InjectVector0, InjectVector
InjectFile = new File("CaSpikeProtocol.dat")
InjectFile.ropen()
InjectMatrix = new Matrix()
InjectMatrix.scanf(InjectFile,500001,2)
InjectVector = new Vector(500001)
for(i=0; i<InjectVector.size-1; i = i+1){
if(i>=InjectMatrix.nrow){
InjectVector.x[i] = -95
}else{
InjectVector.x[i]=InjectMatrix.x[i][1]
}
}
proc init_VClamp() {
access dend[0]
pulse1 = new SEClamp(0.5)
pulse1.dur1 = tstop
InjectVector.play(&pulse1.amp1,dt)
}
proc init_VClamp1() {
access dend[1]
pulse2 = new SEClamp(0.5)
pulse2.dur1 = tstop
InjectVector.play(&pulse2.amp1,dt)
}
proc init_VClamp2() {
access dend[2]
pulse3 = new SEClamp(0.5)
pulse3.dur1 = tstop
InjectVector.play(&pulse3.amp1,dt)
}
proc init_VClamp3() {
access dend[3]
pulse4 = new SEClamp(0.5)
pulse4.dur1 = tstop
InjectVector.play(&pulse4.amp1,dt)
}
finitialize()
init_VClamp()
init_VClamp1()
init_VClamp2()
init_VClamp3()
run()