load_file("stdgui.hoc")
load_file("morphology_mechanisms_DM.hoc")
xopen("BuffersNPumps.ses")
objref pulse, pulse1, pulse2
objref InjectMatrix
objref InjectFile, InjectVector0, InjectVector
InjectFile = new File("CaSpikeProtocol.dat")
InjectFile.ropen()
InjectMatrix = new Matrix()
InjectMatrix.scanf(InjectFile,500001,2)
InjectVector = new Vector(500001)
for(i=0; i<InjectVector.size-1; i = i+1){
if(i>=InjectMatrix.nrow){
InjectVector.x[i] = -95
}else{
InjectVector.x[i]=InjectMatrix.x[i][1]
}
}
tstop = InjectVector.size
dt = 0.02
tstop = 2000
proc init_VClamp() {
access dend[0]
pulse = new SEClamp(0.5)
pulse.dur1 = tstop
InjectVector.play(&pulse.amp1,dt)
}
proc init_VClamp1() {
access dend[1]
pulse1 = new SEClamp(0.5)
pulse1.dur1 = tstop
InjectVector.play(&pulse1.amp1,dt)
}
proc init_VClamp2() {
access dend[2]
pulse2 = new SEClamp(0.5)
pulse2.dur1 = tstop
InjectVector.play(&pulse2.amp1,dt)
}
Vrest = -90
celsius = 24 //degrees
v_init = Vrest
steps_per_ms = 1/dt
t = 0 // simulation starts at t = 0 ms
finitialize()
init_VClamp()
init_VClamp1()
init_VClamp2()
run()