load_file("../templates/Pyram_WT_modif.tem")
load_file("../templates/FS_WT_modif.tem")
begintemplate string //Equivalent to ronalds string handler
public str
strdef str
proc init(){
str=$s1
}
endtemplate string
objref word,word1, word3,word4,rand_gen,cells,nil,stim_list
rand_gen=new Random(34)//seeds 34,78569783335
strdef end
word=new string("")
word1=new string("")
use_mcell_ran4(1)
strdef num_flag
n_types=2 //number of cell types indx1==0 =>pyramidal indx1==1 =>fast spiking
//sgr subgrps of spikes to a giiven population
//NL=> number of pyramidal/fast spiking layers
for(Cell_type=0;Cell_type<n_types;Cell_type+=1){
if(Cell_type==0){NL=n_layerP end="P" sgr=sub_spkE}else{if(Cell_type==1){NL=n_layerFS end="FS" sgr=sub_spkI}}
for(idx_spk=0;idx_spk<=sgr;idx_spk+=1){
sprint(word.str, "objref Spikesource%s%g[%g],excit_syn%s%g[%g],Ic%s[%g]",end,idx_spk,NL,end,idx_spk,NL,end,NL)
execute(word.str)
//print word.str
for Ly=0,NL-1{
sprint(word1.str,"Spikesource%s%g[%g]=new List() excit_syn%s%g[%g]=new List() Ic%s[%g]=new List()",end,idx_spk,Ly,end,idx_spk,Ly,end,Ly)
execute(word1.str)
}
}
}
obfunc createSpikeGen(){local ctr,i,ii,interv,numb,start,noise,inf,inf1,max, max1,cell_type,layer localobj target_cells
strdef end1
target_cells=$o1 interv=$2 numb=$3/*<= not in use*/ start=$4 noise=$5 excit_v=$6 excit_tau=$7
inf=$8 max=$9 cell_type=$10 layer=$11 syn_cond=$12 sub_idx=$13
word3=new string("")
if(target_cells.count()>0){
for i=0,target_cells.count()-1 target_cells.o(i).soma{
inf1=target_cells.o(i).Area()*inf // arbitrary scale factor
max1=target_cells.o(i).Area()*max // arbitrary scale factor
if(cell_type==0){end1="P"}else{if(cell_type==1){end1="FS"}}
for(ctr=0;ctr<=sub_idx;ctr+=1){
if(ctr==1){interv=$14 noise=1 syn_cond=$15 //attention!!! this incorporates "RANDOM" additional spike-trains to the measumerents
//print "additional syn in cell_type"," ",cell_type," interval, noise and syn_weight ", interv," ",noise," ",syn_cond
}
sprint(word3.str,"Spikesource%s%g[%g].append(new NetStim(0.5))",end1,ctr,layer)
execute(word3.str)
//sprint(word3.str,"Spikesource%s%g[%g].o(%g).interval=int(tstop/(%g))",end1,ctr,layer,i,numb)
sprint(word3.str,"Spikesource%s%g[%g].o(%g).interval=%g",end1,ctr,layer,i,interv)
execute(word3.str)
sprint(word3.str,"Spikesource%s%g[%g].o(%g).number=int(tstop/(%g))",end1,ctr,layer,i,interv)
//sprint(word3.str,"Spikesource%s%g[%g].o(%g).number=%g",end1,ctr,layer,i,numb)
execute(word3.str)
sprint(word3.str,"Spikesource%s%g[%g].o(%g).start=%g",end1,ctr,layer,i,start)
execute(word3.str)
sprint(word3.str,"Spikesource%s%g[%g].o(%g).noise=%g",end1,ctr,layer,i,noise)
execute(word3.str)
sprint(word3.str,"excit_syn%s%g[%g].append(new ExpSyn(0.5))",end1,ctr,layer)//exponential synapse to apply the stimulus
execute(word3.str)
sprint(word3.str,"excit_syn%s%g[%g].o(%g).e=%g",end1,ctr,layer,i,excit_v)
execute(word3.str)
sprint(word3.str,"excit_syn%s%g[%g].o(%g).tau=%g",end1,ctr,layer,i,excit_tau)
execute(word3.str)
//print word3.str
}
}
}
for ii=0,target_cells.count()-1 {
if(target_cells.count()-1>0){
if(cell_type==0){end1="P"}else{if(cell_type==1){end1="FS"}}
thresh=0 delay=1 Excit_weight=syn_cond //uS
for(ctr=0;ctr<=sub_idx;ctr+=1){
sprint(word3.str,"layer%s[%g].o(%g).netcon.append(new NetCon(Spikesource%s%g[%g].o(%g),excit_syn%s%g[%g].o(%g),%g,%g,%g))",end1,layer,ii,end1,ctr,layer,ii,end1,ctr,layer,ii,thresh,delay,Excit_weight)
execute(word3.str)
}
sprint(word3.str,"setup_IC_soma(layer%s[%g],%g,%g,%g,%g,%g)",end1,layer,ii,inf1,max1,cell_type,layer)
execute(word3.str)
//print word3.str
//setup_IC_soma(target_cells,i,inf1,max1,cell_type,layer)
}
}
return target_cells
}
proc setup_IC_soma(){local i,inf, max,unif_rand,cell_type,layer localobj Stim_in
Stim_in=$o1 i=$2 inf=$3 max=$4 cell_type=$5 layer=$6 unif_rand=rand_gen.uniform(inf,max)
word4=new string("")
strdef end2
if(cell_type==0){end2="P"}else{if(cell_type==1){end2="FS"}}
Stim_in.o(i).soma {
sprint(word4.str,"Ic%s[%g].append(new IClamp(0.5))",end2,layer)
execute(word4.str)
sprint(word4.str,"Ic%s[%g].o(%g).dur=tstop",end2,layer,i)
//sprint(word4.str,"Ic%s[%g].o(%g).dur=tstop/2",end2,layer,i)
execute(word4.str)
if(cell_type==1){
sprint(word4.str,"Ic%s[%g].o(%g).amp=%g",end2,layer,i,unif_rand)//=>cell_type =0 for pyramidal population
execute(word4.str)
}else if (cell_type==0){
sprint(word4.str,"Ic%s[%g].o(%g).amp=%g",end2,layer,i,unif_rand)
execute(word4.str)}
//print "The iclamp amplitude is",word4.str//=> cell_type=1 for fs_cell
sprint(word4.str,"Ic%s[%g].o(%g).del=100",end2,layer,i)
execute(word4.str)
/*
sprint(word4.str,"Ic%s[%g].o(%g).freq=10",end2,layer,i)
execute(word4.str)*/
}
}