This is the readme for the modeling in the paper: Kamiya H (2022) Modeling analysis of subthreshold voltage signaling along hippocampal mossy fiber axons. Front. Cell. Neurosci., https://www.frontiersin.org/articles/10.3389/fncel.2022.966636 In this paper, the models of the sodium, potassium, and calcium channels found in the hippocampal mossy fiber terminals were reconstructed according to the parameters described in the papers; Engel D, Jonas P (2005) Presynaptic action potential amplification by voltage-gated Na+ channels in hippocampal mossy fiber boutons. Neuron 45: 405-17. http://dx.doi.org/10.1016/j.neuron.2004.12.048 and Li L, Josef Bischofberger J, Jonas P (2007) Differential gating and recruitment of P/Q-, N-, and R-type Ca2+ channels in hippocampal mossy fiber boutons. J. Neurosci. 27: 13420-9. https://www.jneurosci.org/content/27/49/13420 To test for the subthreshold axonal voltage signaling on spike propagation and transmitter release, these models of axonal sodium, potassium, and calcium channel were implemented with the structure model of mossy fiber and granule cells as described in; Alle H, Geiger JR (2006) Combined analog and action potential coding in hippocampal mossy fibers. Science, 311: 1290-3. https://www.science.org/doi/10.1126/science.1119055 To run the simulations, follow these steps: Compile the mod files using nrnivmodl under Mac OS X and Linux, or mknrndll under Windows. Run mosinit.hoc in NEURON. Click the radiobuttons in the panel "Demo" and click "Init & Run" in the "RunControl" panel to run simulations. Questions about this model should be directed to kamiya@med.hokudai.ac.jp.