TITLE Low threshold calcium current
:
: Ca++ current responsible for low threshold spikes (LTS)
:
: Written by Alain Destexhe, Salk Institute, Sept 18, 1992
: Modified by Geir Halnes, Norwegian University of Life Sciences, June 2011:
:
: - Kinetics adapted to LGN interneuron data from Broicher et al.: Mol Cell Neurosci 36: 132-145, 2007.
: using Q10 values of 3 and 1.5 for activation/inactivation.
: - Activation variable shifted 8mV to account for dLGN interneuron data in Halnes et al. 2011
INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)}
NEURON {
THREADSAFE
SUFFIX it2
USEION Ca READ Cai, Cao WRITE iCa VALENCE 2
RANGE gcabar, g
GLOBAL m_inf, tau_m, h_inf, tau_h, shift2, sm, sh, phi_m, phi_h, hx, mx, shift1
}
UNITS {
(molar) = (1/liter)
(mV) = (millivolt)
(mA) = (milliamp)
(mM) = (millimolar)
FARADAY = (faraday) (coulomb)
R = (k-mole) (joule/degC)
}
PARAMETER {
v (mV)
celsius = 36 (degC)
gcabar = 8.5e-6 (mho/cm2)
hx = 1.5
mx = 3.0
Cai = 5e-5 (mM) : Initial Ca concentration
Cao = 2 (mM) : External Ca concentration
: GH, parameters fitted to Broicher et al. 07 - data
minf1 = 46.2
hinf1 = 69.7
taum1 = 5.4
taum2 = 125.7
taum3 = -19.7
taum4 = -0.54
taum5 = 13
tauh1 = 21
tauh2 = 22.2
tauh3 = 9.1
tauh4 = 362.9
tauh5 = 46.9
sm = 8.7
sh = 6.4
shift1 = -8 (mV) : Halnes et al. 2011
shift2 = 0 (mV) : Halnes et al. 2011
}
STATE {
m h
}
ASSIGNED {
iCa (mA/cm2)
g (mho/cm2)
carev (mV)
m_inf
tau_m (ms)
h_inf
tau_h (ms)
phi_m
phi_h
}
BREAKPOINT {
SOLVE castate METHOD cnexp
g = gcabar * m*m*h
iCa = g * ghk(v, Cai, Cao)
}
DERIVATIVE castate {
evaluate_fct(v)
m' = (m_inf - m) / tau_m
h' = (h_inf - h) / tau_h
}
UNITSOFF
INITIAL {
VERBATIM
Cai = _ion_Cai;
Cao = _ion_Cao;
ENDVERBATIM
:
phi_m = mx ^ ((celsius-23.5)/10)
phi_h = hx ^ ((celsius-23.5)/10)
evaluate_fct(v)
m = m_inf
h = h_inf
}
PROCEDURE evaluate_fct(v(mV)) {
m_inf = 1.0 / ( 1 + exp(-(v+shift1+minf1)/sm) )
h_inf = 1.0 / ( 1 + exp((v+shift2+hinf1)/sh) )
tau_m = (taum1+1.0/(exp((v+shift1+taum2)/(taum3))+exp((v+shift1+taum4)/taum5)))/ phi_m
tau_h = (tauh1+1/(exp((v+shift2+tauh2)/tauh3)+exp(-(v+shift2+tauh4)/tauh5)))/phi_h
}
FUNCTION ghk(v(mV), Ci(mM), Co(mM)) (.001 coul/cm3) {
LOCAL z, eci, eco
z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))
eco = Co*efun(z)
eci = Ci*efun(-z)
:high Cao charge moves inward
:negative potential charge moves inward
ghk = (.001)*2*FARADAY*(eci - eco)
}
FUNCTION efun(z) {
if (fabs(z) < 1e-4) {
efun = 1 - z/2
}else{
efun = z/(exp(z) - 1)
}
}
UNITSON