// The goal of this experiment is to insure that our cell responses
// are reasonably similar to the data on back propagating action potentials
// where the site of current injection is either at the soma or the apical trunk
load_proc("nrnmainmenu") // load main NEURON library
cvode.active(1)
load_template("ExperimentControl") // load a custom made library function that centralizes parameters so as to
strdef accstr // not confuse experimental variable bindings with neurophysiological variable bindings
objref econ // Create an experiment object
show_errs=1
debug_lev=1
econ=new ExperimentControl(show_errs,debug_lev)
econ.self_define(econ) // points the object at itself
econ.morphology_dir = "../../morphology/n123" // Setup morphology directory
econ.generic_dir = "../../experiment" // Setup directory with cell-setup file
econ.add_lib_dir("Terrence","../../lib") // Setup directory with library functions
econ.data_dir = "data" // Define directory to save produced data
sprint(econ.syscmd, "mkdir -p %s", econ.data_dir) // make the data directory
system(econ.syscmd)
// Setup cell
econ.xopen_geometry_dependent("cell") // load the raw cell morphology
econ.xopen_geometry_dependent("cell-analysis") // load user-defined semantics on morphology
cell_analysis(econ)
printf("Opening cell setup\n")
econ.xopen_generic("cell-setup") // load the cell-setup file (define specific
printf("Opened. Setting up cell\n") // channels, membrane properties etc)
cell_setup(econ)
// Set simulation parameters for the experiment
econ.defvar("Simulation Control", "tstop", "750", "Defines when the simulation stops.")
econ.defvar("Simulation Control", "dt", "0.1", "Timestep")
econ.defvar("Simulation Control", "steps_per_ms", "10", "How many points are plotted per ms")
setdt()
// Define directories for different cases tested
strdef data_dir
data_dir = "data/BPAP/Soma" // Define directory to save produced data for somatic stimulation
sprint(econ.syscmd, "mkdir -p %s", data_dir) // make directory
system(econ.syscmd)
// Insert current clamp into soma or dendritic section
access soma
//access apical_dendrite[62] //stimulate for dendritic bpap train
//access apical_dendrite[64] //stimulate for single-Ca++-dendritic spike in apical_dendrite[64]
objref ic,ic2
ic = new IClamp(0.5)
// Configure current clamp
econ.defvar("Current Clamp Control", "ic.del", "50", "Determines the delay before onset of the current clamp.")
econ.defvar("Current Clamp Control", "ic.dur", "700", "Determines the duration of the current clamp.")
// current used with somatic stimulation
econ.defvar("Current Clamp Control", "ic.amp", ".16", "Determines the amplitude of the current clamp at the soma.")
// current used with dendrite 62 stimulation, for dendritically induced trains of BPAP
//econ.defvar("Current Clamp Control", "ic.amp", ".64", "Determines the amplitude of the current clamp at debdrite [64].")
//econ.defvar("Current Clamp Control", "ic.amp", ".48", "Determines the amplitude of the current clamp at debdrite [64].")
// current used with dendrite 64 stimulation, single Ca++ spike
//econ.defvar("Current Clamp Control", "ic.amp", ".48", "Determines the amplitude of the current clamp at debdrite [64].")
//econ.defvar("Current Clamp Control", "ic.dur", "700", "Determines the duration of the current clamp.")
// Create basic graphics
econ.xopen_library("Terrence","basic-graphics") // open graphics library file
// section used in train-with-single-Ca++-spike figure
//addgraph_2("apical_dendrite[64].v(0.5)", 0,tstop,-72,20) // 233.67 um from soma
//sections used in BPAP figure
addgraph_2("soma.v(0.5)", 0,tstop,-72,20)
addgraph_2("apical_dendrite[46].v(0.5)",0,tstop,-72,20) // 156.96 um from soma
addgraph_2("apical_dendrite[71].v(0.5)", 0,tstop,-72,20) // 324.53 um from soma
// Initialize and run the experiment
finitialize(v_init)
//mystates.restore()
fcurrent()
cvode.re_init()
run()
// Dump the graphical output to .eps files
//econ.xopen_library("Terrence","verbose-system")
for i=0,windex {
sprint(econ.tmp_str, "%s/graph-%d.eps",data_dir, i) // define the name of the .eps file
win[i].printfile(econ.tmp_str)
}