//--------------------------------------------- PRUNING --------------------------------------------------
if (Apical_pruning){
if (ratio_Pruning==1){
remove_sections(TP_apical_list,L_Pruning)
}else{
random_remove_sections(TP_apical_list,ratio_Pruning,L_Pruning_ob,L_Pruning_tu,ob,vector_distance_Apic_TP_b)
}
}
if (Basal_pruning){
if (ratio_Pruning==1){
remove_sections(TP_basal_list,L_Pruning)
}else{
random_remove_sections(TP_basal_list,ratio_Pruning,0,L_Pruning_b,0,vector_distance_Basal_TP_b)
}
}
//--------------------------------------------End PRUNING--------------------------------------------------
print "GGGGGGGGGGGGGGGGGGGGGGGGGGGG AFTER PRUNING GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG"
//------------------AGAIN------------------ To calculate the morphological parameter --------------------------
n_soma=n_section("soma")
n_dend=n_section("dendrite")
n_apic=n_section("apical_dendrite")
print "Number of sections in\n soma: ",n_soma," dendrite: ",n_dend," apical dendrite: ",n_apic
dendrite_n_list= n_list(basal_tree_list)
apical_dentrite_n_list=n_list(apical_non_trunk_list)
print "Number of real elements(axon and trunk removed) of:"
print " drendrites: ",dendrite_n_list," apical dendrites: ",apical_dentrite_n_list
Terminal_Parent(apical_non_trunk_list,apical_trunk_list,"Apical dendrites",display_Apical_Dendrite_TP)
matrix_apical_dendrite_TP = matrix_coord.c()
vector_L_Apic_TP=vector_L.c
TP_apical_list=TP_list
Terminal_Parent(basal_tree_list,soma_list,"Dedrites",display_Dendrite_TP)
matrix_dendrite_TP = matrix_coord.c()
vector_L_Basal_TP=vector_L.c
TP_basal_list=TP_list
Branch_Child_Parent(apical_non_trunk_list,apical_trunk_list,"Apical dendrites",display_Apical_Dendrite_BP)
matrix_apical_dendrite_BP = matrix_coord.c()
BP_apical_list=BP_list
Branch_Child_Parent(basal_tree_list,soma_list,"Dedrites",display_Dendrite_BP)
matrix_dendrite_BP = matrix_coord.c()
BP_basal_list=BP_list
//---------------AGAIN---------------------END To calculate the morphologycal parameter --------------------------
//---------------AGAIN------------------- To calculate the radial disance to de center of the soma -----------
distanceTosoma(matrix_apical_dendrite_TP,xcg,ycg,zcg,"Distance apical dendrite TP",display_Radial_Distance_Apical_Dendrite_TP)
vector_distance_Apic_TP_b=vector_distance.c
distanceTosoma(matrix_dendrite_TP,xcg,ycg,zcg,"Distance basal dendrite TP",display_Radial_Distance_Dendrite_TP)
vector_distance_Basal_TP_b=vector_distance.c
distanceTosoma(matrix_apical_dendrite_BP,xcg,ycg,zcg,"Distance apical dendrite BP",display_Radial_Distance_Apical_Dendrite_BP)
vector_distance_Apic_BP_b=vector_distance.c
distanceTosoma(matrix_dendrite_BP,xcg,ycg,zcg,"Distance basal dendrite BP",display_Radial_Distance_Dendrite_BP)
vector_distance_Basal_BP_b=vector_distance.c
//-----------------------Shell-wise Dendrite Path Length for pruned neuron---------------------------
Sholl_Path_Length(apical_non_trunk_list,R,dR,xcg,ycg,zcg)
PL_Sholl_apical_b=PL_Sholl
Sholl_Path_Length(basal_tree_list,R,dR,xcg,ycg,zcg)
PL_Sholl_basal_b=PL_Sholl
PL_Sholl_apical_b=PL_Sholl_apical_b.reverse
xy2.erase()
xy2.beginline()
for(k=-R/dR;k<0;k+=1){
xy2.line((k)*dR,PL_Sholl_apical_b.x[k+R/dR])
}
xy2.line(0,0) //I put this point
for(k=1;k<(R/dR);k+=1){
xy2.line((k)*dR,PL_Sholl_basal_b.x[k-1])
}
xy2.flush()
//---------------AGAIN-----------------------------To calculate the Dendritic path lenght ---------------------
lenghtTosoma(TP_apical_list)
L_TP_apical_b=L_dend.c
lenghtTosoma(BP_apical_list)
L_BP_apical_b=L_dend.c
lenghtTosoma(TP_basal_list)
L_TP_basal_b=L_dend.c
lenghtTosoma(BP_basal_list)
L_BP_basal_b=L_dend.c
xopen_filehoc(root_lib,"histographBP_TP02b_button") ///ESTO HAY QUE CAMBIARLO, CREAR OTRO FICHERO PARA ESTE CASO
//----------AGAIN-----------To calculate the combined Dendritic length for pruned neuron----------------------
DL1_b=(combined_DL(TP_apical_list)+combined_DL(BP_apical_list)) // Total apical dendritic length
DL2_b=(combined_DL(TP_basal_list)+combined_DL(BP_basal_list)) // Total basal dendritic length
// Total apical trunk length is the same
DL_b=DL1_b+DL2_b+DL3
reduced_DL1=(DL1_a-DL1_b)*100/DL1_a
reduced_DL2=(DL2_a-DL2_b)*100/DL2_a
reduced_DL=(DL_a-DL_b)*100/DL_a
print "Combined dendritic lengths"
print "\nApical dendritic length ", DL1_a, "in pruned neuron: ",DL1_b , " % : ", reduced_DL1
print "Basal dendritic length ",DL2_a, "in pruned neuron: ",DL2_b , " % : ", reduced_DL2
print "Apical trunk length ",DL3
print "\nTotal dendritic length ",DL_a, "in pruned neruon: ",DL_b, " % :",reduced_DL
//------------------------------------------ Display NEURON-------------------------------------------------
display.color_list(apical_trunk_list,6)
display.color_list(axon_sec_list,3)
display.color_list(soma_list,2)
display.color_list(basal_tree_list,4)
display.color_list(apical_non_trunk_list,5)
display.flush()
//------------------------------------------End Display NEURON----------------------------------------------