Continuum model of tubulin-driven neurite elongation ---------------------------------------------------- This model investigates the elongation over time of a single developing neurite (axon or dendrite). The dynamics of outgrowth, determined by numerical integration of the model, are explored in the paper (to be referred to as GLM): Bruce P. Graham, Karen Lauchlan and Douglas R. McLean, "Dynamics of outgrowth in a continuum model of neurite elongation", Journal of Computational Neuroscience, to appear. Our neurite growth model describes the elongation of a single, unbranched neurite in terms of the rate of extension of the microtubule cytoskeleton. The cytoskeleton is not explicitly modelled, but its construction is assumed to depend on the available free tubulin at the growing neurite tip. A site of tubulin production in the cell body (soma) results in a flux of tubulin across the soma/neurite interface from where tubulin is transported along the neurite by active transport and diffusion. A constant, slow active transport rate is assumed. At the distal neurite tip tubulin is consumed by microtubule assembly, which represents the average assembly of all available microtubules. If assembly exceeds disassembly, then the population of microtubules increases in length, on average. To incorporate the effect of somatic tubulin concentration on tubulin synthesis into the model, the flux of tubulin across the soma/neurite interface may be continuously autoregulated by the tubulin concentration there. Finally, tubulin is assumed everywhere to degrade with a fixed time constant. For simplicity we assume that this single ``degradation'' process encompasses degradation of the tubulin dimers, a constant consumption of tubulin by microtubule assembly along the length of the neurite, and also allows for local synthesis of new tubulin in the neurite leading to an apparent increase in the tubulin half-life. Microtubule assembly proximal to the neurite tip is assumed not to contribute to neurite elongation. Full mathematical details of the model and the algorithm for its numerical solution using a finite difference scheme are given in GLM. A novelty here is that the neurite length changes dynamically, so stretching of the spatial discretization must be incorporated. Steady-state analysis of the model can be found in: McLean & Graham, Proc. Roy. Soc. Lond. A. 460:2437-2456, 2004. McLean, Lauchlan & Graham, WSEAS Trans. Biol. and Biomed. 2:98-105, 2005. The numerical code was developed using Matlab version 7. Example Main Matlab file: CMNG_exampmain.m GUI Version: CMNG_gui.fig and CMNG_gui.m Runnable m-files for each figure in the JCN paper e.g. GLMpap_Figs2_3.m etc