strdef cmd
objref volt_cl
objref vbox1, vbox2, vbox3, vbox4, vbox5
objref g_v, g_i1, g_i2, g_c
objref v_vec, nctr_vec, tr_vec
objref ms
v_vec = new Vector()
nctr_vec = new Vector()
tr_vec = new Vector()
y_min = -1.0
create soma
soma {
diam = 21.851
L = 21.851
nseg = 1
cm = 1
Ra = 60
insert CaR
cai0_ca_ion = 0.000001
cao0_ca_ion = 4
celsius = 22
volt_cl = new VClamp_plus(.5)
}
vbox5 = new VBox()
vbox5.intercept(1)
ms = new MechanismStandard("CaR")
ms.action("change_CaR()")
ms.in()
ms.panel()
vbox5.intercept(0)
vbox5.map("CaR Control", 1, 854, -1, -1)
proc change_CaR() {
ms.out()
}
proc Clamp() {
volt_cl.amp[1]=$1
volt_cl.amp[0]=hold_pot
volt_cl.amp[2]=hold_pot
soma_curr_tr=0
finitialize(v_init)
pre_i = 0
while (t<tstop) {
dens=soma.ica(.5)
g_v.line(t, soma.v(.5))
g_v.flush()
g_i1.line(t, dens)
g_i1.flush()
if ((t>volt_cl.dur[0]) && (t<(volt_cl.dur[0]+volt_cl.dur[1]))) {
if (abs(dens)>abs(pre_i)) {
execute(cmd)
soma_curr_tr=dens
}
}
fadvance()
pre_i=dens
}
v_vec.append($1)
nctr_vec.append(soma_curr_tr)
tr_vec.append(soma_curr_tr)
doEvents()
}
proc erase() {
g_v.erase(0)
g_i1.erase(0)
g_v.size(0, tstop, hold_pot-10, end_cl+10)
g_i1.size(0, tstop, y_min, 0.1)
g_v.beginline()
g_i1.beginline(2,1)
}
proc start() {
v_vec.resize(0)
nctr_vec.resize(0)
tr_vec.resize(0)
g_i2.erase(0)
g_i2.size(st_cl, end_cl, y_min, 0.1)
g_i2.beginline(2,1)
g_v.erase()
g_i1.erase()
g_v.size(0, tstop, hold_pot-10, end_cl+10)
g_i1.size(0, tstop, y_min, 0.1)
for (i=st_cl; i<=end_cl; i=i+incr1) {
erase()
Clamp(i)
tr_vec.line(g_i2,v_vec,2,1)
}
aa = nctr_vec.max()
for i=0, v_vec.size()-1 {
nctr_vec.x[i]=nctr_vec.x[i]/aa
}
g_c.erase()
g_c.size(st_cl,end_gr,0,1)
g_c.begin()
for i=0, v_vec.size()-1 {
if (v_vec.x[i]==end_gr) {
v_vec.resize(i)
nctr_vec.resize(i)
break
}
}
nctr_vec.line(g_c,v_vec,1,1)
g_c.flush()
doNotify()
}
proc Tstop() {
tstop = volt_cl.dur[0]+volt_cl.dur[1]+volt_cl.dur[2]
}
access soma
execute(cmd)
{
xpanel("Run Control", 0)
v_init = -80
xvalue("Init","v_init", 1,"stdinit()", 1, 1 )
xbutton("Init & Run","run()")
xbutton("Stop","stoprun=1")
runStopAt = 5
xvalue("Continue til","runStopAt", 1,"{continuerun(runStopAt) stoprun=1}", 1, 1 )
runStopIn = 1
xvalue("Continue for","runStopIn", 1,"{continuerun(t + runStopIn) stoprun=1}", 1, 1 )
xbutton("Single Step","steprun()")
t = 40
xvalue("t","t", 2 )
tstop = 415
xvalue("Tstop","tstop", 1,"tstop_changed()", 0, 1 )
dt = 0.125
xvalue("dt","dt", 1,"setdt()", 0, 1 )
steps_per_ms = 40
xvalue("Points plotted/ms","steps_per_ms", 1,"setdt()", 0, 1 )
screen_update_invl = 0.05
xvalue("Scrn update invl","screen_update_invl", 1,"", 0, 1 )
realtime = 0.00999999
xvalue("Real Time","realtime", 0,"", 0, 1 )
xpanel(1,102)
}
{
xpanel("Clamp Control", 0)
xbutton("Start","start()")
y_min = -0.2
xvalue("maximal current", "y_min")
st_cl=-80
xvalue("Start voltage", "st_cl")
end_cl=60
xvalue("End voltage", "end_cl")
end_gr=60
xvalue("end graph", "end_gr")
incr1=1
volt_cl.dur[0]=5
xvalue("Pre-pulse duration", "volt_cl.dur[0]", 1, "Tstop()")
volt_cl.dur[1]=400
xvalue("Test pulse duration", "volt_cl.dur[1]", 1, "Tstop()")
volt_cl.dur[2]=10
xvalue("Post-pulse duration", "volt_cl.dur[2]", 1, "Tstop()")
hold_pot=v_init
xvalue("Holding voltage", "hold_pot")
xpanel(1,537)
}
vbox1=new VBox()
vbox1.intercept(1)
g_v=new Graph()
g_v.size(0, tstop, hold_pot-10, end_cl+10)
vbox1.intercept(0)
vbox1.map("Voltage protocol", 319, 24, 625, 260)
vbox2=new VBox()
vbox2.intercept(1)
g_i1=new Graph()
g_i1.size(0, tstop, y_min, 0.1)
vbox2.intercept(0)
vbox2.map("IV currents", 320, 456, 625, 304)
vbox3=new VBox()
vbox3.intercept(1)
g_i2=new Graph()
g_i2.size(st_cl, end_cl, y_min, 0.1)
vbox3.intercept(0)
vbox3.map("IV-relationship", 1161, 454, 306, 298)