/*----------------------------------------------------------------------------
Calyx-of-Held to MNTB Model - Pre and Postsynaptic Structures
-------------------------------------------------------------
B. Graham, Dept. of Computing Science & Maths, University of Stirling
(Contact: b.graham@cs.stir.ac.uk)
(previously IANC, Division of Informatics, University of Edinburgh)
CNS 2000 Version (19/11/02)
----------------------------------------------------------------------------*/
create AXON, MNTB
N=500 // number of release sites (same as RSIZE in coh2.mod)
objectvar coh // presynaptic calyx of Held
objectvar c[1] // postsynaptic receptor sites
//----------------------------------------------------------------------------
// create AMPA receptors
//----------------------------------------------------------------------------
proc recampa() {
objectvar c[N] // postsynaptic receptor sites
for i = 0, N-1 {
c[i] = new AMPA()
MNTB c[i].loc(0.5) // assign postsynaptic compartment
setpointer c[i].C, coh.T[i] // connect presynaptic transmitter
c[i].gmax = 500 // (pS) maximum conductance
}
}
proc setgmax() {
for i = 0, N-1 {c[i].gmax = $1} // (pS) maximum conductance
}
//----------------------------------------------------------------------------
// create pre and postsynaptic compartments and insert passive properties
//----------------------------------------------------------------------------
forall {
diam=20
L=20
Ra=200
insert pas
g_pas=1/50000
e_pas=v_init
}
//----------------------------------------------------------------------------
// create presynaptic and insert postsynaptic mechansisms
//----------------------------------------------------------------------------
// calyx of Held
coh = new COH()
AXON coh.loc(0.5)
// postsynaptic receptors
recampa()
objectvar vcl // voltage-clamp object
if (postAP == 1) { // generate postsynaptic spikes
access MNTB
v_init=-66
MNTB {
insert BKDNaDR gnabar_BKDNaDR=0.025 gkbar_BKDNaDR=0.03
g_pas=1/500
e_pas=v_init
}
} else { // record postsynaptic currents
// voltage clamp postsynaptic cell
MNTB vcl = new VClamp(.5)
vcl.dur[0] = 11000
vcl.amp[0] = v_init
}