back=0
//////// load needed templates////////////
if(!back){ load_file("nrngui.hoc") }
if(!back){ load_file("template/ObliquePath.hoc") }
if(!back){ load_file("template/BasalPath.hoc" ) }
objref econ,f1,f2,f3,ss,cvode
f1= new File()
f2= new File()
f3= new File()
ss=new SaveState()
cvode = new CVode(1)
x=cvode.active(1)
restart=0
v_init=-70
strdef morphology_location, morpho_path, ObliqueTrunkSection, BasalTrunkSection
objref vRP, vAPEX
proc xopen_morphology(){
sprint(morpho_path,"%s/%s",morphology_location,$s1)
xopen(morpho_path)
}
// Carmen
morphology_location = "pc2b"
ObliqueTrunkSection = "trunk[17]"
BasalTrunkSection = "trunk[7]"
xopen_morphology("cell.hoc") //reads morpholofy from the file
xopen_morphology("cell-analysis-simple.hoc") //reads simplified version of CA
// --------------- Creating lists-----------------
xopen("lib/TP-lib.hoc")
Tip_sections(apical_non_trunk_list,apical_trunk_list,"Apical")
objref apical_tip_list
apical_tip_list=TP_list // Apical Tip list
print "apical_tip_list"
apical_tip_list.printnames()
print "END apical_tip_list"
objref tmp_pl[num_tips],pl[num_tips],opl[num_tips],degree_apical_tip,peri_trunk_list
objref bl[num_tips],obl[num_tips],degree_basal_tip
xopen("lib/Oblique-lib.hoc")
oblique_sections(apical_tip_list,apical_trunk_list,num_tips) // apical dendrite path lists and degree of tips
xopen("lib/vector-distance.hoc")
//-----------------------------------------------------------------------------------------------------
printf("Setting up cell\n") // load cell-setup to
xopen("cell-setup.hoc") // specify all mechanisms, membrane properties etc
///////////////////Load File//////////////////////
//if(!back) load_file("kca.ses")
if(!back) { load_file("print.ses") }
/*cvode_active(1)*/
////// Spike counter//////////
objref apc, v1
proc insert_APC() {
apc = new APCount(0.5)
apc.thresh = $1
v1 = new Vector()
apc.record(v1)
}
proc init() {
if(restart){
f1.ropen("state.old")
ss.fread(f1)
f1.close
finitialize(v_init)
ss.restore()
t=0
fcurrent()
cvode.re_init()
} else {
finitialize(v_init)
fcurrent()
}
}
////////////////////Main///////////////////
nsyn=10
objref s[nsyn], rsyn[nsyn], nc[nsyn]
objref rsynmda[nsyn], ncnmda[nsyn]
objref tvec,ampvec,iclamp
proc main(){
tstop=4000
in=0
th=-14
access soma
insert_APC(th)
f3.wopen("spike.dat")
current=0.0 // 0.18
npulse = 10
period = 10 // 10
pulsdur = 2
pulsamp = 1.0
starttime = 1000
soma {
iclamp = new IClamp( 0.5 )
tvec = new Vector( npulse * 2 )
ampvec = new Vector( npulse * 2 )
}
iclamp.del = starttime
iclamp.amp = 0
for i=0,(npulse-1){
tvec.x[i*2 ] = starttime + i*period
tvec.x[i*2+1] = starttime + i*period + pulsdur
iclamp.dur = i*period + pulsdur
ampvec.x[i*2 ] = pulsamp
ampvec.x[i*2+1] = 0
}
ampvec.play(&iclamp.amp,tvec)
access soma[0]
//////////////run////////////////////////
run()
/////////////////////////////////////
// print the spike number
if(!back) printf("\n Current: %.4f nA | # Spikes: %d\n", current, apc.n)
if(!back) printf("___________________\n")
v1.printf(f3)
f3.close
f2.wopen("state.new")
ss.save
ss.fwrite(f2)
f2.close
}
main()
/******** end file ******/