: xtrau.mod, based on xtra.mod,v 1.3 2009/02/24 00:52:07
: the "u" stands for "uniform" as in "uniform field"
: because this modification was made to accommodate a
: uniform extracellular field,
: as would occur between two parallel plates
COMMENT
This mechanism is intended to be used in conjunction
with the extracellular mechanism. Pointers specified
at the hoc level must be used to connect the
extracellular mechanism's e_extracellular and i_membrane
to this mechanism's ex and im, respectively.
xtrau does four useful things:
1. Serves as a target for Vector.play() to facilitate
extracellular stimulation. Assumes that one has initialized
a Vector to hold the time sequence of the extracellular field
strength E.
This Vector is to be played into the GLOBAL variable E
(GLOBAL so only one Vector.play() needs to be executed),
which is multiplied by the RANGE variable d ("distance
between the local node and the zero potential plane")
and then by -1.
That product, called ex in this mechanism, is the extracellular
potential at the local node, i.e. is used to drive local
e_extracellular.
2. Reports the contribution of local i_membrane to the
total signal that would be picked up by an extracellular
recording electrode. This is computed as the product of
rx (transfer resistance between the local node and the
monopolar recording electrode), i_membrane (called im in
this mechanism), and the surface area of the local segment,
and is reported as er. The total extracellularly recorded
potential is the sum of all er_xtrau over all segments in
all sections, and is to be computed at the hoc level,
e.g. with code like
func fieldrec() { local sum
sum = 0
forall {
if (ismembrane("xtrau")) {
for (x,0) sum += er_xtrau(x)
}
}
return sum
}
Bipolar recording, i.e. recording the difference in potential
between two extracellular electrodes, can be achieved with no
change to either this NMODL code or fieldrec(); the values of
rx will reflect the difference between the potentials at the
recording electrodes caused by the local membrane current, so
some rx will be negative and others positive.
3. Enables local storage of xyz coordinates interpolated from
the pt3d data. These coordinates are used by hoc code that
computes the transfer resistance that couples the membrane
to extracellular stimulating and recording electrodes.
4. Enables local storage of the distance d of internal
nodes from the zero potential plane.
Prior to NEURON 5.5, the SOLVE statement in the BREAKPOINT block
used METHOD cvode_t so that the adaptive integrators wouldn't miss
the stimulus. Otherwise, the BREAKPOINT block would have been called
_after_ the integration step, rather than from within cvodes/ida,
causing this mechanism to fail to deliver a stimulus current
when the adaptive integrator is used.
With NEURON 5.5 and later, this mechanism abandons the BREAKPOINT
block and uses the two new blocks BEFORE BREAKPOINT and
AFTER BREAKPOINT, like this--
BEFORE BREAKPOINT { : before each cy' = f(y,t) setup
ex = is*rx*(1e6)
}
AFTER SOLVE { : after each solution step
er = (10)*rx*im*area
}
This ensures that the stimulus potential is computed prior to the
solution step, and that the recorded potential is computed after.
ENDCOMMENT
NEURON {
SUFFIX xtrau
RANGE rx, er, d
RANGE x, y, z
GLOBAL E
POINTER im, ex
}
PARAMETER {
: default transfer resistance between recording electrode and node
rx = 1 (megohm) : mV/nA
x = 0 (1) : spatial coords
y = 0 (1)
z = 0 (1)
}
ASSIGNED {
v (millivolts)
: is (milliamp)
E (volt/m) : field strength
d (micron) : distance of node from zero potential plane
ex (millivolts)
im (milliamp/cm2)
er (microvolts)
area (micron2)
}
INITIAL {
: ex = is*rx*(1e6)
ex = -E*d*(1e-3)
er = (10)*rx*im*area
: this demonstrates that area is known
: UNITSOFF
: printf("area = %f\n", area)
: UNITSON
}
: Use BREAKPOINT for NEURON 5.4 and earlier
: BREAKPOINT {
: SOLVE f METHOD cvode_t
: }
:
: PROCEDURE f() {
: : 1 mA * 1 megohm is 1000 volts
: : but ex is in mV
: ex = E*d*(1e-3)
: er = (10)*rx*im*area
: }
: With NEURON 5.5 and later, abandon the BREAKPOINT block and PROCEDURE f(),
: and instead use BEFORE BREAKPOINT and AFTER BREAKPOINT
BEFORE BREAKPOINT { : before each cy' = f(y,t) setup
: ex = is*rx*(1e6)
ex = -E*d*(1e-3)
}
AFTER SOLVE { : after each solution step
er = (10)*rx*im*area
}