<StimulationSet> <!-- the injectionSite must match a regionLabel defined in the morphology --> <!-- 20 Glu and Ca pulses / sec for 1 sec * 20 trains = 400 pulses --> <!--first burst of Da - full strength --> <!-- pulse 1 to produce 0.5 uM alone --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 30000 </onset> <duration> 15 </duration> <rate> 18 </rate> <period> 50 </period> <end> 30020 </end> </InjectionStim> <!-- pulse 2 = half of pulse 1 --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 30050 </onset> <duration> 15 </duration> <rate> 9.0 </rate> <period> 50 </period> <end> 30070 </end> </InjectionStim> <!-- pulse 3 = half of pulse 2 --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 30100 </onset> <duration> 15 </duration> <rate> 6.3 </rate> <period> 50 </period> <end> 30120 </end> </InjectionStim> <!-- pulses 4-20 = 2/3 of ctrl Da, to produce smaller peak than theta, because 40 pulses of 20hz are needed to produce same amount as 40 theta pulses--> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 30150 </onset> <duration> 15 </duration> <rate> 3 </rate> <period> 50 </period> <end> 30999 </end> </InjectionStim> <!--later bursts of Da - partial strength (2/3d) --> <!-- pulse 1 to produce 0.5 uM alone --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 40000 </onset> <duration> 15 </duration> <rate> 12 </rate> <period> 50 </period> <end> 40020 </end> </InjectionStim> <!-- pulse 2 = half of pulse 1 --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 40050 </onset> <duration> 15 </duration> <rate> 6.0 </rate> <period> 50 </period> <end> 40070 </end> </InjectionStim> <!-- pulse 3 = half of pulse 2 --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 40100 </onset> <duration> 15 </duration> <rate> 4.2 </rate> <period> 50 </period> <end> 40120 </end> </InjectionStim> <!-- pulses 4-20 = 2/3 of ctrl Da, faster injection to decrease stochasticity--> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 40150 </onset> <duration> 15 </duration> <rate> 2 </rate> <period> 50 </period> <end> 40999 </end> </InjectionStim> <!-- third and subsequent trains even smaller --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 50000 </onset> <duration> 15 </duration> <rate> 2.0 </rate> <period> 50 </period> <end> 50999 </end> </InjectionStim> <!-- fourth and subsequent trains smaller still --> <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA"> <onset> 60000 </onset> <duration> 15 </duration> <rate> 1.0 </rate> <period> 50 </period> <end> 60999 </end> <numTrains> 5 </numTrains> <interTrainInterval> 9001 </interTrainInterval> </InjectionStim> <!-- basal ACh value prior to and after stim --> <InjectionStim specieID="ACh" injectionSite="dend:submembrane"> <onset> 0 </onset> <duration> 30000 </duration> <rate> 0.2 </rate> </InjectionStim> <InjectionStim specieID="ACh" injectionSite="dend:submembrane"> <onset> 110000 </onset> <duration> 200000 </duration> <rate> 0.2 </rate> </InjectionStim> <!-- basal ACh stim paused during Da stim--> <InjectionStim specieID="ACh" injectionSite="dend:submembrane"> <onset> 33200 </onset> <duration> 6800 </duration> <rate> 0.2 </rate> <period> 10000 </period> <end> 110000 </end> </InjectionStim> <!-- burst during stim : Assume that ACh neurons can follow 20 Hz --> <InjectionStim specieID="ACh" injectionSite="sa1[0].pointA"> <onset> 30000 </onset> <duration> 15 </duration> <rate> 3.0 </rate> <period> 50 </period> <end> 30999 </end> <numTrains> 8 </numTrains> <interTrainInterval> 9001 </interTrainInterval> </InjectionStim> <!-- Should this stimulation be eliminated???--> <InjectionStim specieID="ACh" injectionSite="dend:submembrane"> <onset> 30000 </onset> <duration> 15 </duration> <rate> 3.0 </rate> <period> 50 </period> <end> 30999 </end> <numTrains> 8 </numTrains> <interTrainInterval> 9001 </interTrainInterval> </InjectionStim> <InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA"> <onset> 30000 </onset> <duration> 5 </duration> <rate> 1.125 </rate> <period> 50 </period> <end> 30999 </end> <numTrains> 8 </numTrains> <interTrainInterval> 9001 </interTrainInterval> </InjectionStim> <InjectionStim specieID="Ca" injectionSite="sa1[0].pointA"> <onset> 30000 </onset> <duration> 3 </duration> <rate> 312 </rate> <period> 50 </period> <end> 30999 </end> <numTrains> 8 </numTrains> <interTrainInterval> 9001 </interTrainInterval> </InjectionStim> <InjectionStim specieID="Ca" injectionSite="dend:submembrane"> <onset> 30000 </onset> <duration> 3 </duration> <rate> 156 </rate> <period> 50 </period> <end> 30999 </end> <numTrains> 8 </numTrains> <interTrainInterval> 9001 </interTrainInterval> </InjectionStim> </StimulationSet>