<StimulationSet>
<!-- the injectionSite must match a regionLabel defined in the morphology -->
<!-- one dendrite injection, sa2[:], is distributed over all the spine
(each spine gets total/num spines)
four Glu and Ca pulses at 50 Hz, repeated 10x= 40 pulses per train -->
<!-- This Da is single pulse, representing 4 pulse burst, burst 1 amp = 2x 1st pulse of 20 Hz
longer duration of inject provides for the >100msegion rise time-->
<!-- the 15 s repetition of train provides the first burst for each of 10 trains -->
<InjectionStim specieID="AIP" injectionSite="dend:submembrane">
<onset> 2000 </onset>
<duration> 60 </duration>
<rate> 45 </rate>
</InjectionStim>
<InjectionStim specieID="AIP" injectionSite="sa1[0].pointA">
<onset> 2000 </onset>
<duration> 60 </duration>
<rate> 25 </rate>
</InjectionStim>
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 20000 </onset>
<duration> 60 </duration>
<rate> 6.0 </rate>
<period> 105 </period>
<end> 20100 </end>
</InjectionStim>
<!-- These next Da are single pulse, represent 4 pulse burst; burst 2-10 amp = 1/2 amp of 1st burst -->
<!-- the 15 s repetition of train provides bursts 2-10 for each of 10 trains -->
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 20095 </onset>
<duration> 60 </duration>
<rate> 2.5 </rate>
<period> 95 </period>
<end> 20949 </end>
</InjectionStim>
<!-- bursts 2-10 are lower amplitude than 1st burst -->
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 35000 </onset>
<duration> 60 </duration>
<rate> 4.0 </rate>
<period> 105 </period>
<end> 35100 </end>
<numTrains> 9 </numTrains>
<interTrainInterval> 14900 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 35095 </onset>
<duration> 60 </duration>
<rate> 1.5 </rate>
<period> 95 </period>
<end> 35949 </end>
<numTrains> 9 </numTrains>
<interTrainInterval> 14051 </interTrainInterval>
</InjectionStim>
<!-- basal ACh value prior to and after stim -->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 0 </onset>
<duration> 20000 </duration>
<rate> 0.2 </rate>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 170000 </onset>
<duration> 200000 </duration>
<rate> 0.2 </rate>
</InjectionStim>
<!-- basal ACh stim paused during Da stim-->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 23200 </onset>
<duration> 11800 </duration>
<rate> 0.2 </rate>
<period> 15000 </period>
<end> 170000 </end>
</InjectionStim>
<!-- burst during stim specified for each Glu/Da train (below)-->
<!-- rebound burst: develops more slowly -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 22400 </onset>
<duration> 800 </duration>
<rate> 0 </rate>
<period> 900 </period>
<end> 23299 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 14101 </interTrainInterval>
</InjectionStim>
<!-- *****************TRAIN 1 *** Glu inject **********-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 20000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 20066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- Ca pulses TRAIN 1: 40 Ca pulses -->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 20000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 20066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 20000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 20066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 20000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 20066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 20000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 20066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Glu *******TRAIN 2************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 35000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 35066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 2******* 80 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 35000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 35066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 35000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 35066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 35000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 35066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 35000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 35066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--*********Glu******TRAIN 3***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 50000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 50066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 3******* 120 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 50000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 50066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 50000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 50066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 50000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 50066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 50000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 50066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Glu******TRAIN 4******************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 65000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 65066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 4******* 160 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 65000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 65066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 65000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 65066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 65000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 65066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 65000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 65066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--*********Glu******TRAIN 5***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 80000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 80066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 5******* 200 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 80000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 80066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 80000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 80066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 80000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 80066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 80000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 80066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Glu******TRAIN 6******************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 95000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 95066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 6******* 240 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 95000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 95066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 95000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 95066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 95000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 95066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 95000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 95066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--*********Glu******TRAIN 7***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 110000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 110066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 7******* 280 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 110000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 110066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 110000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 110066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 110000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 110066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 110000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 110066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--*********Glu******TRAIN 8***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 125000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 125066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 8******* 320 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 125000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 125066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 125000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 125066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 125000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 125066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 125000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 125066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--*********Glu******TRAIN 9***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 140000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 140066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 9******* 360 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 140000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 140066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 140000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 140066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 140000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 140066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 140000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 140066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--*********Glu******TRAIN 10***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 155000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 155066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 10******* 320 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 155000 </onset>
<duration> 3 </duration>
<rate> 154 </rate>
<period> 20 </period>
<end> 155066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 155000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 155066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 155000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 155066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 155000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 155066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
</StimulationSet>