<StimulationSet>
<!-- the injectionSite must match a regionLabel defined in the morphology -->
<!-- one dendrite injection, sa2[:], is distributed over all the spine
(each spine gets total/num spines)
four Glu and Ca pulses at 50 Hz, repeated 10x= 40 pulses per train -->
<!-- This Da is single pulse, representing 4 pulse burst, burst 1 amp = 2x 1st pulse of 20 Hz
longer duration of inject provides for the >100msegion rise time-->
<!-- the 15 s repetition of train provides the first burst for each of 10 trains -->
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 30000 </onset>
<duration> 60 </duration>
<rate> 6.0 </rate>
<period> 105 </period>
<end> 30100 </end>
</InjectionStim>
<!-- These next Da are single pulse, represent 4 pulse burst; burst 2-10 amp = 1/2 amp of 1st burst -->
<!-- the 15 s repetition of train provides bursts 2-10 for each of 10 trains -->
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 30095 </onset>
<duration> 60 </duration>
<rate> 2.5 </rate>
<period> 95 </period>
<end> 30949 </end>
</InjectionStim>
<!-- bursts 2-4 are lower amplitude than 1st burst -->
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 45000 </onset>
<duration> 60 </duration>
<rate> 4.0 </rate>
<period> 105 </period>
<end> 45100 </end>
<numTrains> 3 </numTrains>
<interTrainInterval> 14900 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
<onset> 45095 </onset>
<duration> 60 </duration>
<rate> 1.5 </rate>
<period> 95 </period>
<end> 45949 </end>
<numTrains> 3 </numTrains>
<interTrainInterval> 14051 </interTrainInterval>
</InjectionStim>
<!-- basal ACh value prior to and after stim -->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 0 </onset>
<duration> 30000 </duration>
<rate> 0.2 </rate>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 85000 </onset>
<duration> 230000 </duration>
<rate> 0.2 </rate>
</InjectionStim>
<!-- basal ACh stim paused during Da stim-->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 33200 </onset>
<duration> 11800 </duration>
<rate> 0.2 </rate>
<period> 15000 </period>
<end> 90000 </end>
</InjectionStim>
<!-- *****************TRAIN 1 *** Glu inject **********-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 30000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 30066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- Ca pulses TRAIN 1: 40 Ca pulses -->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 30000 </onset>
<duration> 3 </duration>
<rate> 308 </rate>
<period> 20 </period>
<end> 30066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 30000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 30066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 30000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 30066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 30000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 30066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Glu *******TRAIN 2************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 45000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 45066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 2******* 80 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 45000 </onset>
<duration> 3 </duration>
<rate> 308 </rate>
<period> 20 </period>
<end> 45066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 45000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 45066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 45000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 45066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 45000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 45066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--*********Glu******TRAIN 3***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 60000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 60066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 3******* 120 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 60000 </onset>
<duration> 3 </duration>
<rate> 308 </rate>
<period> 20 </period>
<end> 60066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 60000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 60066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 60000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 60066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 60000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 60066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Glu******TRAIN 4******************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
<onset> 75000 </onset>
<duration> 5 </duration>
<rate> 1.125 </rate>
<period> 20 </period>
<end> 75066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!--**********Calcium******TRAIN 4******* 160 total Ca pulses ***********-->
<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
<onset> 75000 </onset>
<duration> 3 </duration>
<rate> 308 </rate>
<period> 20 </period>
<end> 75066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
<onset> 75000 </onset>
<duration> 3 </duration>
<rate> 156 </rate>
<period> 20 </period>
<end> 75066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
<onset> 75000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 75066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
<onset> 75000 </onset>
<duration> 15 </duration>
<rate> 3.0 </rate>
<period> 40 </period>
<end> 75066 </end>
<numTrains> 10 </numTrains>
<interTrainInterval> 29 </interTrainInterval>
</InjectionStim>
</StimulationSet>