// mitral.tem // Template for n-compartment mitral cell model // Modele avec Longue dendrite begintemplate Mit public soma, glom, prim, dend, AMPA, GABAA, spiketimes, spikecount, gsp, gpg create soma, glom, prim, dend[100], s2d, s2p, p2g objref AMPA, GABAA[100], spikecount, spiketimes proc init() { local Len, Erest, RM, p, q, r, Atotal, AMPAtau, AMPArev, GABAAtau, GABAArev create soma, glom, prim, dend[$1], s2d, s2p, p2g spiketimes = new Vector() lastspikecount = 0 Len = 100 Erest = -65 // mV Atotal = 100000 // um2 gsp = 5.47e-11 // S/cm2 gpg = 5.86e-11 // RM = 100000 // ohm.cm2 p = 0.051 q = 0.084 r = 0.328 AMPAtau = 10 //ms AMPArev = 0 //mV GABAAtau = 6 // from Sc&98 GABAArev = -70 // soma { insert pas insert nafast insert kfasttab insert kslowtab insert kA insert kca insert lcafixed insert cad depth_cad = 8 L = Len diam = p*Atotal/(PI*Len) Ra = PI*diam*diam/(4*Len*Atotal) e_pas = Erest // reversal potential mV g_pas = 1/RM // membrane conductance siemen-cm^-2 gnabar_nafast = 0.1532 gkbar_kfasttab = 0.1956 gkbar_kslowtab = 0.0028 gkbar_kA = 0.00587 gkbar_kca = 0.0142 gcabar_lcafixed = 0.0040 spikecount = new APCount(0.5) spikecount.thresh = -30 spikecount.record(spiketimes) } glom { insert pas insert kslowtab insert lcafixed insert cad L = Len diam = q*Atotal/(PI*Len) Ra = PI*diam*diam/(4*Len*Atotal) e_pas = Erest g_pas = 1/RM gkbar_kslowtab = 0.02 gcabar_lcafixed = 0.0095 AMPA = new ExpSyn(0.5) AMPA.tau = AMPAtau AMPA.e = AMPArev } prim { insert pas insert nafast insert kfasttab insert kslowtab insert lcafixed insert cad depth_cad = 8 L = Len diam = r*Atotal/(PI*Len) Ra = PI*diam*diam/(4*Len*Atotal) e_pas = Erest g_pas = 1/RM gkbar_kfasttab = 0.00123 gnabar_nafast = 0.00134 gkbar_kslowtab = 0.00174 gcabar_lcafixed = 0.0022 } for i=0,$1-1 { dend[i] { insert pas insert kfasttab insert nafast L = 10 // um instead of 100 diam = 17.03 //instead of (1-p-q-r)*Atotal/(PI*Len) // um Ra = 595 //1130 //2267 //850 //to keep an electronic length similar e_pas = Erest g_pas = 1/RM gnabar_nafast = 0.033 // 0.0226 gkbar_kfasttab = 0.0226 // 0.0330 GABAA[i] = new ExpSyn(0.5) GABAA[i].tau = GABAAtau GABAA[i].e = GABAArev } } s2p { diam = 1 Ra = PI*diam*diam/(4*Len*Atotal) * ( 1/gsp ) L = 1 } p2g { diam = 1 Ra = PI*diam*diam/(4*Len*Atotal) * ( 1/gpg ) L = 1 } soma connect s2p(0),0 s2p connect prim(0),1 prim connect p2g(0),1 p2g connect glom(0),1 soma connect dend[0](0),1 for i= 0, $1-2 { dend[i] connect dend[i+1](0),1 } // set reversal potentials, etc. forall if (ismembrane("ca_ion")) { eca = 70 // mV cai = 0.00001 // mM cao = 2 // mM ion_style("ca_ion",3,2,0,0,1) } forall if (ismembrane("na_ion")) { ena = 45 // mV } forall if (ismembrane("k_ion")) { ek = -70 // mV } } endtemplate Mit