// this sets the variables used by LGMD_model files, funcs and procs.
// When creating new procedures, use local variables when possible.
// As needed add new global variables to the list below
// ---- Global strdef
// there are no local strdefs in nrn
strdef NRNDIR // NRNDIR is base directory of model
strdef syndir // syndir is subdirectory containing synapse files
strdef esynfullpath // filepath of excitatory synapse file
strdef isynfullpath // filepath of inhibitory synapse file
strdef DATADIR, datadir_ // DATADIR is full path of directory where simulation data is saved
strdef subdir // data subdirectory
strdef strtmp, tmpstr // tmp strings used by several procs & funcs (there are no local strdef in hoc)
strdef datafile // name of data file being saved
strdef filename
strdef AECmap
strdef headerstr // header at top of data file
strdef cwd // working directory
strdef sect
strdef Hname, KDname
KDname = "KD"
Hname = "h"
// ------
// ---- Global objref
objref StrFx // StringFunction object
objref esyn, isyn // lists of excitatory and inhibitory synapse objects
objref espont, ispont // Lists of spontaneous synapse objects
esyn = new List()
isyn = new List()
objref syn, synlist // List of all synapse objects inserted
synlist = new List()
objref synfilelist // List of synapse filenames to include in batch sims
objref sref // SectionRef
objref MS_ // MechanismStandard
objref KDchan,Hchan,Mchan,CaTchan,KAchan // chanAnalysis objects for logging mean conductance
objref AREAS
objref GLS
objref objtmp[1]
// SectionList objects to subdivide the LGMD
objref Ctines,Chandle,InhEnds
objref ParentBranch,ChildBranch
objref FieldA, TineBase
objref TineEnds
objref siz
objref KDList, HList, MList, CaTList, KAList
HList = new SectionList()
KDList = new SectionList()
KAList = new SectionList()
MList = new SectionList()
CaTList = new SectionList()
Ctines = new SectionList() // Field C branches
Chandle = new SectionList() // neurite connecting Field C to Handle
InhEnds = new SectionList() // End compartments of Field B and C branches
ParentBranch = new SectionList() //
ChildBranch = new SectionList()
//FindBranches( ParentBranch, ChildBranch, FieldA)
TineBase = new SectionList()
// FindFieldBase( TineBase, "MainTrunk", "Tines" )
TineEnds = new SectionList()
// FindEnds(TineEnds,"Tines")
MakeSecList( siz, "soma", "SIZ")
MakeSecList( FieldA, "MainTrunk", "Tines")
// I use the '_G_' suffix for many global objects to decrease the chances of name conflicts
objref idc_G_[2] // Array of step currents (IClamp objects) used for dc holding current
objref sp_G_[5] // Array of sum pulses (sEPSP objects) used for summation sims
objref cw_G_ // Chirp current (chirp object) used for ZAP measure sims
objref simsecs_G_ // SectionList specifying sections from which to record simulation data
objref rvec_G_[1], tvec_G_ // time and data vectors to record during simulation
objref ivec_G_ // current vector to record
objref gvec_G_ // conductance and state vectors to record during simulation
objref vecList // list of vectors (currently unused)
RecDt = 0.2 // Sample interval (ms) for saving recorded data to file (should be multiple of dt)
Gstep = 5 // How many time steps between measuring conductances
idc_G_[1] = new IClamp()
// Tines[1] idc_G_[1].loc(0.5)
idc_G_[1].loc(0.5)
idc_G_[1].amp=0
tvec_G_ = new Vector()
tvec_G_.record(idc_G_[1], &t, RecDt)
ivec_G_ = new Vector()
ivec_G_.label("nA")
gvec_G_ = new List()
// cw_G_ = new chirp()
// ------
// ***** Setup input and output directory paths *****
// system("echo $NRNBASE", NRNDIR) // retrieve path of local neuron simulation directory
// StrFx = new StringFunctions()
// strlen = StrFx.len(NRNDIR)
// StrFx.left(NRNDIR,strlen-1) // remove newline character at end of string
NRNDIR = getcwd()
NRNDIR = "."
strtmp="date \"+%m%d%Y\" "
system(strtmp,tmpstr) // get current date
StrFx = new StringFunctions()
strlen = StrFx.len(tmpstr)
StrFx.left(tmpstr,strlen-1) // remove newline character at end of string
// make directory to save data
datadir_ = "data/" // data directory is named "data"
sprint( DATADIR, "%s/%s%s", NRNDIR, datadir_, tmpstr )
sprint(strtmp, "mkdir -p %s", DATADIR)
system(strtmp)
sprint( syndir, "%s/synapse", NRNDIR )
// syndir = "/Users/rich/Documents/MATLAB/syns_spontaneous/0708/"
// ***** End Setup input and output directory paths *****
/* --- Initialize Global variables for LGMD simulation --- */
loadsyn=2 // state. whether to load synapse files for visual stimulus. 0=none, 1=set file, 2=set directory
seclist=2 // state. sets which sections to add to SecList used by simulations
synCa = 0 // state. synapse causes Ca influx (value enumerates different effects of Ca influx)
recCa = 0 // boolean. whether to record cai instead of v
acn = 0 // boolean. whether the moddel has cyclic nucleotide dependent mechs
presyn=1 // boolean. Wether SimBatch procedure preloads synapses before starting sim
AEC = 0 // boolean. Whether the morphology has been reduced into electrotonically equivalent cable(s)
// tstart = 350 // time to start simulations (ms). Allows model to reach steady state
e4AP = 0.90 // effectiveness of 4AP application (0 = no effect, 1 = complete block)
eZD = 0.95 // effectiveness of ZD7288 application (0 = no effect, 1 = complete block)
eXE = 0.95 // effectiveness of XE991 application (0 = no effect, 1 = complete block)
tstart=0 // the time (ms into simulation) that stimulus starts
t0=0 // the time of collision for visual stimuli
nSection=0 // number of sections in the model
forall nSection+=1
AREAS = new Vector(nSection)
n=0
forall {
AREAS.x[n] = area(0.5)
n+=1
}
SA = AREAS.sum() // total surface area of cell
/* --- end simulation globals */
/* --- Initialize Global membrane variables --- */
passive=0 // state. 0=full model, 1=no active conductances, 2=passive dendrites, 3=h and pas dendrites, 4=passive siz
gl = 9.0e-6 // leak conductance (S/cm2) (for FieldA, some other sections set higher)
el = -65 // leak reversal potential (mV)
Cm = 0.80 // membrane capacitance (µF/cm2)
axial = 350 // axial resistivity (Ωcm) (some sections ignore this value)
SIZNa = 0.17 // sodium channel density at siz (S/cm2) (other sections scaled to this value)
SIZKdr = 2.0e-2 // Kdr channel density at siz (S/cm2) (other sections scaled to this value)
SIZM = 1.0e-3 // M channel density at siz (S/cm2) (other sections scaled to this value)
EK = -77 // K+ reversal potential (mV)
ENa = 50 // Na+ reversal potential (mV)
ECa = 100 // initial Ca2+ reversal potential (mV), changes with Ca2+ influx and eflux
Rabeforeg = 2 // 0: set condunctance then axial resistivity gradients, 1: set Ra then g, 2: no Ra gradient
// in locust saline (mM): [Na] = 144, [K] = 5, [Ca]=5, [Cl]=157