"""
STIMULI
This code defines properties of different type of stimuli
and defines functions to generate the stimuli
"""
from pylab import array, rand, exp, zeros, hstack
import shared as s # Import population and connection data
#PMd, ASC, DSC, ER2, IF2, IL2, ER5, EB5, IF5, IL5, ER6, IF6, IL6, AMPA, NMDA, GABAA, GABAB, opsin, Epops, Ipops, allpops = cpd.names2inds() # Define populations
# Class with natural touch stimulus
class touch:
name = 'touch'
receptor = s.AMPA # Set which receptor to stimulate
isi = 0.5 # Interstimulus interval in s
var = 0.0 # Variation in ISI in s
width = 0.005 # Stimulus width in s
weight = 100 # Weight of stimuli
sta = [] # Starting time in s -- usually defined below
fin = [] # Finishing time in s
shape = 'gaussian' # Shape of stimulus
#pops = [TCR]
fraction = 1 # No idea what this should be...
rate = 200 # 500 is the highest rate that can be used without significantly slowing down the simulation
noise = 0 # Variability in onset time
loc = array([[0.4,0.6],[0.4,0.6]]) # Location of cells to be stimulated
falloff = [array([0.5,0.5]), 1e9] # Weight fall-off characteristics of the stimulus: center and Gaussian width in um
# class with optogenetic stimulus
class opto:
name = 'opto'
receptor = s.opsin # Set which receptor to stimulate
isi = 0.100 # Interstimulus interval in s
var = 0.0 # Variation in ISI
width = 0.2 # Stimulus width in s
weight = 10 # Weight of stimuli
sta = 3 # Starting time in s
fin = 6 # Finishing time in s
shape = 'square' # Shape of stimulus
pops = [s.ER5, s.EB5, s.ER6]
fraction = 0.4 # No idea what this should be...
rate = 500 # 500 is the highest rate that can be used without significantly slowing down the simulation
noise = 0
loc = array([[0.0,1.0],[0.0,1.0]]) # Location of cells to be stimulated
falloff = [array([0.5,0.5]), 2000] # Weight fall-off characteristics of the stimulus: center and Gaussian width in um
# Allow defaults to be overriden by name, e.g. instead of touch(), stimmod(touch,sta=1,fin=3)
def stimmod(stimclass, name=None, receptor=None, isi=None, var=None, width=None, weight=None, sta=None, fin=None, shape=None, pops=None, fraction=None, rate=None, noise=None, loc=None, falloff=None):
stiminstance = stimclass()
stimproperties = ['name', 'receptor', 'isi', 'var', 'width', 'weight', 'sta', 'fin', 'shape', 'pops', 'fraction', 'rate', 'noise', 'loc', 'falloff'];
for prop in stimproperties:
thisproperty = eval(prop)
if thisproperty!=None:
exec('stiminstance.' + prop + ' = thisproperty')
return stiminstance
## Define stimulus-making code
def makestim(isi=1, variation=0, width=0.05, weight=10, start=0, finish=1, stimshape='gaussian'):
from pylab import r_, convolve, shape
# Create event times
timeres = 0.005 # Time resolution = 5 ms = 200 Hz
pulselength = 10 # Length of pulse in units of width
currenttime = 0
timewindow = finish-start
allpts = int(timewindow/timeres)
output = []
while currenttime<timewindow:
if currenttime>=0 and currenttime<timewindow: output.append(currenttime)
currenttime = currenttime+isi+variation*(rand()-0.5)
# Create single pulse
npts = min(pulselength*width/timeres,allpts) # Calculate the number of points to use
x = (r_[0:npts]-npts/2+1)*timeres
if stimshape=='gaussian':
pulse = exp(-(x/width*2-2)**2) # Offset by 2 standard deviations from start
pulse = pulse/max(pulse)
elif stimshape=='square':
pulse = zeros(shape(x))
pulse[int(npts/2):int(npts/2)+int(width/timeres)] = 1 # Start exactly on time
else:
raise Exception('Stimulus shape "%s" not recognized' % stimshape)
# Create full stimulus
events = zeros((allpts))
events[array(array(output)/timeres,dtype=int)] = 1
fulloutput = convolve(events,pulse,mode='same')*weight # Calculate the convolved input signal, scaled by rate
fulltime = (r_[0:allpts]*timeres+start)*1e3 # Create time vector and convert to ms
fulltime = hstack((0,fulltime,fulltime[-1]+timeres*1e3)) # Create "bookends" so always starts and finishes at zero
fulloutput = hstack((0,fulloutput,0)) # Set weight to zero at either end of the stimulus period
events = hstack((0,events,0)) # Ditto
stimvecs = [fulltime, fulloutput, events] # Combine vectors into a matrix
return stimvecs