//genesis /*************************** MS Model, Version 9.1 ********************* **************************** CaR.g ********************* Rebekah Evans 3/20/12 updated channel. ****************************************************************************** ******************************************************************************/ function create_CaR str chanName = "CaR_channel" str compPath = "/library" int c = 0 int xdivs = 3000 float Ek = 0.140 //(nernst calculated for 35degrees, [Cain] 50nM [Caout]2mM) //Ek is overwritten the the GHK object if it is used. float x = -0.1 float xmin = -0.1 float xmax = 0.05 float increment ={{xmax}-{xmin}}/{xdivs} echo "CaR increment:" {increment} "V" float mPower = 3.0 //Foerhing et al., 2000 p 2228 float hPower = 1.0 if (calciuminact == 1) float zpower = 1.0 else float zpower = 0 end float mvHalfCaR = -29e-3 float mkCaR = -9.6e-3 float hvHalfCaR = -33.3e-3 float hkCaR = 17e-3 float mTauCaR = 5.1e-003 //Foehring et al., 2000 pg 2230. float mInfCaR = 0.0 float hTauCaR = 0.0 float hInfCaR = 0.0 float hA float hB //parameters for calcium-dep inactivation (CDI) float Ca = 0.0 float CaMax = 0.005 //5uM float CaMin = 0 float CaDivs = 3000 float CaIncrement ={{CaMax}-{CaMin}}/{CaDivs} echo "CDIincrement:" {CaIncrement} float qFactCaR = {qfactCa} float surf = 0.0 float gMax = 0 float theta = 0.0 float theta_exp = 0.0 float beta = 0.0 float beta_exp = 0.0 pushe {compPath} create tabchannel {chanName} setfield {chanName} Ek {Ek} Xpower {mPower} Ypower {hPower} Zpower {zpower} call {chanName} TABCREATE X {xdivs} {xmin} {xmax} call {chanName} TABCREATE Y {xdivs} {xmin} {xmax} for(c = 0; c < {xdivs} + 1; c = c + 1) /************************ Begin CaR_mTau *********************/ // mTauCaR setfield {chanName} X_A->table[{c}] {{mTauCaR}/{qFactCaR}} /************************ End CaR_mTau ***********************/ /************************ Begin CaR_mInf *********************/ //mInfCaR = 1./(1 + exp((vMemb - mvHalfCaR)/mkCaR)); // parameters tuned to fit Foerhing et al., 2000 fig 6 theta = {{x} - {mvHalfCaR}}/{mkCaR} theta_exp = {exp {theta}} + 1.0 mInfCaR = 1.0/{theta_exp} setfield {chanName} X_B->table[{c}] {mInfCaR} /************************ End CaR_mInf ***********************/ /************************ Begin CaR_hTau *********************/ // hA = 10e6*(vMemb + 0.0945)./ // (exp((vMemb + 0.0945)/0.00512)-1); // hB = 84.2*exp(vMemb/0.013); // hTauCaR = ((1/(hA + hB))+0.1) / qFactCaR; // parameters tuned to fit Brevi 2001 fig 10 theta = {{10e6}*{ {x} + 0.0945}} beta = {{x} + 0.0945}/0.00512 beta_exp = {exp {beta}} beta_exp = beta_exp - 1.0 hA = {{theta}/{beta_exp}} beta = {{x}/0.013} beta_exp = {exp {beta}} hB = 84.2*{beta_exp} hTauCaR = {{1.0/{{hA} + {hB}}}+0.02} setfield {chanName} Y_A->table[{c}] {hTauCaR}/{qFactCaR} /************************ End CaR_hTau ***********************/ /************************ Begin CaR_hInf *********************/ //hInfCaR = 1./(1 + exp((vMemb - hvHalfCaR)/hkCaR)); // parameters tuned to fit Foerhing et al., 2000 fig 7 theta = {{x} - {hvHalfCaR}}/{hkCaR} theta_exp = {exp {theta}} + 1.0 hInfCaR = 1.0/{theta_exp} setfield {chanName} Y_B->table[{c}] {hInfCaR} /************************ End CaR_hInf ***********************/ x = x + increment end tweaktau {chanName} X tweaktau {chanName} Y //fill in the Z table with CDI values //equation from Tuckwell review paper 2012 progress in neurobiology table A1.3 if (calciuminact == 1) call {chanName} TABCREATE Z {CaDivs} {CaMin} {CaMax} int a float CDI float q, k, b, n for(a = 0; a < {CaDivs} + 1; a = a + 1) //f= (0.001/(0.001+[Ca]))Poirazi CA1 2003 //f= (0.0005/(0.0005+[Ca])) Rhodes and Llinas 2001 Cort Pyr Ca=a*{CaIncrement} k=0.0005 b={pow {k} 3} n={pow {Ca} 3} q = {{b}/({b}+{n})} CDI = {pow {q} 100} setfield {chanName} Z_B->table[{a}] {CDI} setfield {chanName} Z_A->table[{a}] {{142e-3}/{cdiqfact}} //CaL1.2 in HEK cells Barrett and Tsien 2007 roomtemp end tweaktau {chanName} Z end create ghk {chanName}GHK setfield {chanName}GHK Cout 2 // Carter & Sabatini 2004 uses 2mM, // Wolf 5mM setfield {chanName}GHK valency 2.0 setfield {chanName}GHK T {TEMPERATURE} setfield {chanName} Gbar {gMax*surf} addmsg {chanName} {chanName}GHK PERMEABILITY Gk pope end