:Interneuron Cells to Pyramidal Cells GABA with local Ca2+ pool and read public soma Ca2+ pool
NEURON {
POINT_PROCESS interD2pyrD_STFD
USEION ca READ eca,ica
NONSPECIFIC_CURRENT igaba
RANGE initW
RANGE Cdur_gaba, AlphaTmax_gaba, Beta_gaba, Erev_gaba, gbar_gaba, W, on_gaba, g_gaba
RANGE eca, tauCa, Icatotal
RANGE ICag, P0g, fCag
RANGE Cainf, pooldiam, z
RANGE lambda1, lambda2, threshold1, threshold2
RANGE fmax, fmin, Wmax, Wmin, maxChange, normW, scaleW, srcid, destid,limitW
RANGE pregid,postgid, thr_rp
RANGE F, f, tauF, D1, d1, tauD1, D2, d2, tauD2
RANGE facfactor
RANGE neuroM,type
}
UNITS {
(mV) = (millivolt)
(nA) = (nanoamp)
(uS) = (microsiemens)
FARADAY = 96485 (coul)
pi = 3.141592 (1)
}
PARAMETER {
srcid = -1 (1)
destid = -1 (1)
type = -1
Cdur_gaba = 0.7254 (ms)
AlphaTmax_gaba = 1.52 (/ms):7.2609 (/ms): 2.2609 (/ms): 3.2609 (/ms) : 7.2609 as original
Beta_gaba = 0.14(/ms) : 0.147 (/ms) : 0.2667 (/ms): : 0.2667 as original
Erev_gaba = -75 (mV) : -75 as original
gbar_gaba = 0.6e-3 (uS)
Cainf = 50e-6 (mM)
pooldiam = 1.8172 (micrometer)
z = 2
neuroM = 0
k = 0.01
tauCa = 50 (ms)
P0g = .01
fCag = .024
lambda1 = 1 : 0.7 : 0.6 : 0.7 : 1.0 : 0.5 : 1.5 :3 : 4 : 3 : 2 : 3.0 : 2.0
lambda2 = .01
threshold1 = 0.5 :0.47 : 0.48 : 0.45 : 0.4 : 0.95 : 1.35 :0.75 :0.55 (uM)
threshold2 = 0.6 :0.52 : 0.53 : 0.5 : 0.45 : 1.0 : 1.4 : 0.8 : 0.65 :0.70 (uM)
:GABA Weight
initW = 5.0 : 3.0 : 4.0 : 5.0 : 4.2 : 3.5 :4.5 : : : 3 : 2.5 : 5
fmax = 3 : 2.85 :4 : 3 : 3
fmin = .8
GAPstart1 = 96000
GAPstop1 = 196000
thr_rp = 1 : .7
facfactor = 1
: the (1) is needed for the range limits to be effective
f = 0 (1) < 0, 1e9 > : 1.3 (1) < 0, 1e9 > : facilitation
tauF = 20 (ms) < 1e-9, 1e9 >
d1 = 0.95 (1) < 0, 1 > : fast depression
tauD1 = 40 (ms) < 1e-9, 1e9 >
d2 = 0.9 (1) < 0, 1 > : slow depression
tauD2 = 70 (ms) < 1e-9, 1e9 >
DAstart1 = 39500
DAstop1 = 40000
DAstart2 = 35900
DAstop2 = 36000
DA_t1 = 0.7 : 0.7
DA_t2 = 1.5 : 1.3 : 1.2
DA_t3 = 1.25
DA_S = 1.6 : 1.8 : 1.8
Beta1 = 0.001 (/ms) : 1/decay time for neuromodulators
Beta2 = 0.0001 (/ms)
NEstart1 = 39500
NEstop1 = 40000
NEstart2 = 35900
NEstop2 = 36000
NE_t1 = 1 : 1 : 0.95
NE_t2 = 1 :1 : 0.7 : 0.8
NE_t3 = 1
NE_S = 1 : 0.4
}
ASSIGNED {
v (mV)
eca (mV)
ica (nA)
igaba (nA)
g_gaba (uS)
on_gaba
limitW
t0 (ms)
ICan (nA)
ICag (nA)
Afactor (mM/ms/nA)
Icatotal (nA)
dW_gaba
Wmax
Wmin
maxChange
normW
scaleW
pregid
postgid
rp
tsyn
fa
F
D1
D2
}
STATE { r_nmda r_gaba capoolcon W }
INITIAL {
on_gaba = 0
r_gaba = 0
W = initW
limitW = 1
t0 = -1
Wmax = fmax*initW
Wmin = fmin*initW
maxChange = (Wmax-Wmin)/10
dW_gaba = 0
capoolcon = Cainf
Afactor = 1/(z*FARADAY*4/3*pi*(pooldiam/2)^3)*(1e6)
fa =0
F = 1
D1 = 1
D2 = 1
}
BREAKPOINT {
if ((eta(capoolcon)*(lambda1*omega(capoolcon, threshold1, threshold2)-lambda2*GAP1(GAPstart1, GAPstop1)*W))>0&&W>=Wmax) {
limitW=1e-12
} else if ((eta(capoolcon)*(lambda1*omega(capoolcon, threshold1, threshold2)-lambda2*GAP1(GAPstart1, GAPstop1)*W))<0&&W<=Wmin) {
limitW=1e-12
} else {
limitW=1 }
SOLVE release METHOD cnexp
: if (W >= Wmax || W <= Wmin ) { : for limiting the weight
: limitW=1e-12
: } else {
: limitW=1
: }
:if (W > Wmax) {
:W = Wmax
:} else if (W < Wmin) {
:W = Wmin
:}
if (neuroM==1) {
g_gaba = gbar_gaba*r_gaba*facfactor*DA1(DAstart1,DAstop1)*DA2(DAstart2,DAstop2) : Dopamine effect on GABA
} else if (neuroM==2) {
g_gaba = gbar_gaba*r_gaba*facfactor*NEn(NEstart1,NEstop1)*NE2(NEstart2,NEstop2) : Norepinephrine effect on GABA
} else if (neuroM==3) {
g_gaba = gbar_gaba*r_gaba*facfactor*DA1(DAstart1,DAstop1)*DA2(DAstart2,DAstop2)*NEn(NEstart1,NEstop1)*NE2(NEstart2,NEstop2) : Dopamine & Norepinephrine effect on GABA
} else {
g_gaba = gbar_gaba*r_gaba*facfactor
}
igaba = W*g_gaba*(v - Erev_gaba)
ICag = P0g*g_gaba*(v - eca)
Icatotal = ICag + k*ica*4*pi*((15/2)^2)*(0.01) : icag+k*ica*Area of soma*unit change
}
DERIVATIVE release {
: W' = eta(capoolcon)*(lambda1*omega(capoolcon, threshold1, threshold2)-lambda2*GAP1(GAPstart1, GAPstop1)*W) : Long-term plasticity was implemented. (Shouval et al. 2002a, 2002b)
W' = 1e-12*limitW*eta(capoolcon)*(lambda1*omega(capoolcon, threshold1, threshold2)-lambda2*GAP1(GAPstart1, GAPstop1)*W)
r_gaba' = AlphaTmax_gaba*on_gaba*(1-r_gaba)-Beta_gaba*r_gaba
capoolcon'= -fCag*Afactor*Icatotal + (Cainf-capoolcon)/tauCa
}
NET_RECEIVE(dummy_weight) {
if (flag==0) { :a spike arrived, start onset state if not already on
if ((!on_gaba)){ :this synpase joins the set of synapses in onset state
t0=t
on_gaba=1
net_send(Cdur_gaba,1)
} else if (on_gaba==1) { :already in onset state, so move offset time
net_move(t+Cdur_gaba)
t0=t
}
}
if (flag == 1) { : turn off transmitter, i.e. this synapse enters the offset state
on_gaba=0
}
if (flag == 0) { : Short term plasticity was implemented(Varela et. al 1997):
rp = unirand()
:F = 1 + (F-1)* exp(-(t - tsyn)/tauF)
D1 = 1 - (1-D1)*exp(-(t - tsyn)/tauD1)
D2 = 1 - (1-D2)*exp(-(t - tsyn)/tauD2)
:printf("%g\t%g\t%g\t%g\t%g\t%g\n", t, t-tsyn, F, D1, D2, facfactor)
:printf("%g\t%g\t%g\t%g\n", F, D1, D2, facfactor)
tsyn = t
facfactor = F * D1 * D2
::F = F+f :F * f
if (F > 3) {
F=3 }
if (facfactor < 0.7) {
facfactor=0.7
}
D1 = D1 * d1
D2 = D2 * d2
:printf("\t%g\t%g\t%g\n", F, D1, D2)
}
}
:::::::::::: FUNCTIONs and PROCEDUREs ::::::::::::
FUNCTION eta(Cani (mM)) {
LOCAL taulearn, P1, P2, P4, Cacon
P1 = 0.1
P2 = P1*1e-4
P4 = 1
Cacon = Cani*1e3
taulearn = P1/(P2+Cacon*Cacon*Cacon)+P4
eta = 1/taulearn*0.001
}
FUNCTION omega(Cani (mM), threshold1 (uM), threshold2 (uM)) {
LOCAL r, mid, Cacon
Cacon = Cani*1e3
r = (threshold2-threshold1)/2
mid = (threshold1+threshold2)/2
if (Cacon <= threshold1) { omega = 0}
else if (Cacon >= threshold2) { omega = 1/(1+50*exp(-50*(Cacon-threshold2)))}
else {omega = -sqrt(r*r-(Cacon-mid)*(Cacon-mid))}
}
FUNCTION DA1(DAstart1 (ms), DAstop1 (ms)) {
LOCAL DAtemp1, DAtemp2, DAtemp3, DAtemp4, DAtemp5, DAtemp6, DAtemp7, DAtemp8, DAtemp9, DAtemp10, DAtemp11, DAtemp12, DAtemp13, DAtemp14, DAtemp15, DAtemp16, DAtemp17, DAtemp18, DAtemp19, DAtemp20, DAtemp21, DAtemp22, DAtemp23, DAtemp24, DAtemp25, DAtemp26, DAtemp27, DAtemp28, DAtemp29, DAtemp30, DAtemp31, DAtemp32, DAtemp33, DAtemp34,s
DAtemp1 = DAstart1+4000
DAtemp2 = DAtemp1+4000
DAtemp3 = DAtemp2+4000
DAtemp4 = DAtemp3+4000
DAtemp5 = DAtemp4+4000
DAtemp6 = DAtemp5+4000
DAtemp7 = DAtemp6+4000
DAtemp8 = DAtemp7+4000
DAtemp9 = DAtemp8+4000
DAtemp10 = DAtemp9+4000
DAtemp11 = DAtemp10+4000
DAtemp12 = DAtemp11+4000
DAtemp13 = DAtemp12+4000
DAtemp14 = DAtemp13+4000
DAtemp15 = DAtemp14 + 4000 + 100000 : 100sec Gap
DAtemp16 = DAtemp15 + 4000
DAtemp17 = DAtemp16 + 4000
DAtemp18 = DAtemp17 + 4000
DAtemp19 = DAtemp18 + 4000
DAtemp20 = DAtemp19 + 4000
DAtemp21 = DAtemp20 + 4000
DAtemp22 = DAtemp21 + 4000
DAtemp23 = DAtemp22 + 4000
DAtemp24 = DAtemp23 + 4000
DAtemp25 = DAtemp24 + 4000
DAtemp26 = DAtemp25 + 4000
DAtemp27 = DAtemp26 + 4000
DAtemp28 = DAtemp27 + 4000
DAtemp29 = DAtemp28 + 4000
DAtemp30 = DAtemp29 + 4000
DAtemp31 = DAtemp30 + 4000
DAtemp32 = DAtemp31 + 4000
DAtemp33 = DAtemp32 + 4000
DAtemp34 = DAtemp33 + 4000
if (t <= DAstart1) { DA1 = 1.0}
else if (t >= DAstart1 && t <= DAstop1) {DA1 = DA_t1} : 2nd tone in conditioning
else if (t > DAstop1 && t < DAtemp1) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-DAstop1))} : Basal level
else if (t >= DAtemp1 && t <= DAtemp1+500) {DA1=DA_t1} : 3rd tone
else if (t > DAtemp1+500 && t < DAtemp2) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp1+500)))} : Basal level
else if (t >= DAtemp2 && t <= DAtemp2+500) {DA1=DA_t1} : 4th tone
else if (t > DAtemp2+500 && t < DAtemp3) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp2+500)))} : Basal level
else if (t >= DAtemp3 && t <= DAtemp3+500) {DA1=DA_t1} : 5th tone
else if (t > DAtemp3+500 && t < DAtemp4) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp3+500)))} : Basal level
else if (t >= DAtemp4 && t <= DAtemp4+500) {DA1=DA_t1} : 6th tone
else if (t > DAtemp4+500 && t < DAtemp5) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp4+500)))} : Basal level
else if (t >= DAtemp5 && t <= DAtemp5+500) {DA1=DA_t1} : 7th tone
else if (t > DAtemp5+500 && t < DAtemp6) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp5+500)))} : Basal level
else if (t >= DAtemp6 && t <= DAtemp6+500) {DA1=DA_t1} : 8th tone
else if (t > DAtemp6+500 && t < DAtemp7) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp6+500)))} : Basal level
else if (t >= DAtemp7 && t <= DAtemp7+500) {DA1=DA_t1} : 9th tone
else if (t > DAtemp7+500 && t < DAtemp8) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp7+500)))} : Basal level
else if (t >= DAtemp8 && t <= DAtemp8+500) {DA1=DA_t1} : 10th tone
else if (t > DAtemp8+500 && t < DAtemp9) {DA1 = 1.0 + (DA_t1-1)*exp(-Beta1*(t-(DAtemp8+500)))} : Basal level
else if (t >= DAtemp9 && t <= DAtemp9+500) {DA1=DA_t2} : 11th tone - Second Step
else if (t > DAtemp9+500 && t < DAtemp10) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp9+500)))} : Basal level
else if (t >= DAtemp10 && t <= DAtemp10+500) {DA1=DA_t2} : 12th tone
else if (t > DAtemp10+500 && t < DAtemp11) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp10+500)))} : Basal level
else if (t >= DAtemp11 && t <= DAtemp11+500) {DA1=DA_t2} : 13th tone
else if (t > DAtemp11+500 && t < DAtemp12) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp11+500)))} : Basal level
else if (t >= DAtemp12 && t <= DAtemp12+500) {DA1=DA_t2} : 14th tone
else if (t > DAtemp12+500 && t < DAtemp13) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp12+500)))} : Basal level
else if (t >= DAtemp13 && t <= DAtemp13+500) {DA1=DA_t2} : 15th tone
else if (t > DAtemp13+500 && t < DAtemp14) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp13+500)))} : Basal level
else if (t >= DAtemp14 && t <= DAtemp14+500) {DA1=DA_t2} : 16th tone
else if (t > DAtemp14+500 && t < DAtemp15) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp14+500)))} : Basal level
else if (t >= DAtemp15 && t <= DAtemp15+500) {DA1 = DA_t2} : 1st tone EE
else if (t > DAtemp15+500 && t < DAtemp16) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp15+500)))} : Basal level
else if (t >= DAtemp16 && t <= DAtemp16+500) {DA1 = DA_t2} : 2nd tone EE
else if (t > DAtemp16+500 && t < DAtemp17) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp16+500)))} : Basal level
else if (t >= DAtemp17 && t <= DAtemp17+500) {DA1 = DA_t2} : 3rd tone EE
else if (t > DAtemp17+500 && t < DAtemp18) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp17+500)))} : Basal level
else if (t >= DAtemp18 && t <= DAtemp18+500) {DA1 = DA_t2} : 4th tone EE
else if (t > DAtemp18+500 && t < DAtemp19) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp18+500)))} : Basal level
else if (t >= DAtemp19 && t <= DAtemp19+500) {DA1 = DA_t3} : 5th tone EE
else if (t > DAtemp19+500 && t < DAtemp20) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp19+500)))} : Basal level
else if (t >= DAtemp20 && t <= DAtemp20+500) {DA1 = DA_t3} : 6th tone EE
else if (t > DAtemp20+500 && t < DAtemp21) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp20+500)))} : Basal level
else if (t >= DAtemp21 && t <= DAtemp21+500) {DA1 = DA_t3} : 7th tone EE
else if (t > DAtemp21+500 && t < DAtemp22) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp21+500)))} : Basal level
else if (t >= DAtemp22 && t <= DAtemp22+500) {DA1 = DA_t3} : 8th tone EE
else if (t > DAtemp22+500 && t < DAtemp23) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp22+500)))} : Basal level
else if (t >= DAtemp23 && t <= DAtemp23+500) {DA1 = DA_t3} : 9th tone EE
else if (t > DAtemp23+500 && t < DAtemp24) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp23+500)))} : Basal level
else if (t >= DAtemp24 && t <= DAtemp24+500) {DA1 = DA_t3} : 10th tone EE
else if (t > DAtemp24+500 && t < DAtemp25) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp24+500)))} : Basal level
else if (t >= DAtemp25 && t <= DAtemp25+500) {DA1 = DA_t3} : 11th tone EE
else if (t > DAtemp25+500 && t < DAtemp26) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp25+500)))} : Basal level
else if (t >= DAtemp26 && t <= DAtemp26+500) {DA1 = DA_t3} : 12th tone EE
else if (t > DAtemp26+500 && t < DAtemp27) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp26+500)))} : Basal level
else if (t >= DAtemp27 && t <= DAtemp27+500) {DA1 = DA_t3} : 13th tone EE
else if (t > DAtemp27+500 && t < DAtemp28) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp27+500)))} : Basal level
else if (t >= DAtemp28 && t <= DAtemp28+500) {DA1 = DA_t3} : 14th tone EE
else if (t > DAtemp28+500 && t < DAtemp29) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp28+500)))} : Basal level
else if (t >= DAtemp29 && t <= DAtemp29+500) {DA1 = DA_t3} : 15th tone EE
else if (t > DAtemp29+500 && t < DAtemp30) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp29+500)))} : Basal level
else if (t >= DAtemp30 && t <= DAtemp30+500) {DA1 = DA_t3} : 16th tone EE
else if (t > DAtemp30+500 && t < DAtemp31) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp30+500)))} : Basal level
else if (t >= DAtemp31 && t <= DAtemp31+500) {DA1 = DA_t3} : 17th tone EE
else if (t > DAtemp31+500 && t < DAtemp32) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp31+500)))} : Basal level
else if (t >= DAtemp32 && t <= DAtemp32+500) {DA1 = DA_t3} : 18th tone EE
else if (t > DAtemp32+500 && t < DAtemp33) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp32+500)))} : Basal level
else if (t >= DAtemp33 && t <= DAtemp33+500) {DA1 = DA_t3} : 19th tone EE
else if (t > DAtemp33+500 && t < DAtemp34) {DA1 = 1.0 + (DA_t2-1)*exp(-Beta2*(t-(DAtemp33+500)))} : Basal level
else if (t >= DAtemp34 && t <= DAtemp34+500) {DA1 = DA_t3} : 20th tone EE
else { DA1 = 1.0}
}
FUNCTION DA2(DAstart2 (ms), DAstop2 (ms)) {
LOCAL DA2temp1, DA2temp2, DA2temp3, DA2temp4, DA2temp5, DA2temp6, DA2temp7, DA2temp8, DA2temp9, DA2temp10, DA2temp11, DA2temp12, DA2temp13, DA2temp14, DA2temp15, DA2temp16,s
DA2temp1 = DAstart2 + 4000
DA2temp2 = DA2temp1 + 4000
DA2temp3 = DA2temp2 + 4000
DA2temp4 = DA2temp3 + 4000
DA2temp5 = DA2temp4 + 4000
DA2temp6 = DA2temp5 + 4000
DA2temp7 = DA2temp6 + 4000
DA2temp8 = DA2temp7 + 4000
DA2temp9 = DA2temp8 + 4000
DA2temp10 = DA2temp9 + 4000
DA2temp11 = DA2temp10 + 4000
DA2temp12 = DA2temp11 + 4000
DA2temp13 = DA2temp12 + 4000
DA2temp14 = DA2temp13 + 4000
DA2temp15 = DA2temp14 + 4000
if (t <= DAstart2) { DA2 = 1.0}
else if (t >= DAstart2 && t <= DAstop2) {DA2 = DA_S } : 1st shock
else if (t > DAstop2 && t < DA2temp1) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DAstop2+500)))}
else if (t >= DA2temp1 && t <= DA2temp1+100) {DA2=DA_S} : 2nd shock
else if (t > DA2temp1+100 && t < DA2temp2) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp1+100)))}
else if (t >= DA2temp2 && t <= DA2temp2+100) {DA2=DA_S} : 3rd shock
else if (t > DA2temp2+100 && t < DA2temp3) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp2+100)))}
else if (t >= DA2temp3 && t <= DA2temp3+100) {DA2=DA_S} : 4th shock
else if (t > DA2temp3+100 && t < DA2temp4) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp3+100)))}
else if (t >= DA2temp4 && t <= DA2temp4+100) {DA2=DA_S} : 5th shock
else if (t > DA2temp4+100 && t < DA2temp5) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp4+100)))}
else if (t >= DA2temp5 && t <= DA2temp5+100) {DA2=DA_S} : 6th shock
else if (t > DA2temp5+100 && t < DA2temp6) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp5+100)))}
else if (t >= DA2temp6 && t <= DA2temp6+100) {DA2=DA_S} : 7th shock
else if (t > DA2temp6+100 && t < DA2temp7) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp6+100)))}
else if (t >= DA2temp7 && t <= DA2temp7+100) {DA2=DA_S} : 8th shock
else if (t > DA2temp7+100 && t < DA2temp8) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp7+100)))}
else if (t >= DA2temp8 && t <= DA2temp8+100) {DA2=DA_S } : 9th shock
else if (t > DA2temp8+100 && t < DA2temp9) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp8+100)))}
else if (t >= DA2temp9 && t <= DA2temp9+100) {DA2=DA_S } : 10th shock
else if (t > DA2temp9+100 && t < DA2temp10) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp9+100)))}
else if (t >= DA2temp10 && t <= DA2temp10+100) {DA2=DA_S} : 11th shock
else if (t > DA2temp10+100 && t < DA2temp11) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp10+100)))}
else if (t >= DA2temp11 && t <= DA2temp11+100) {DA2=DA_S } : 12th shock
else if (t > DA2temp11+100 && t < DA2temp12) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp11+100)))}
else if (t >= DA2temp12 && t <= DA2temp12+100) {DA2=DA_S} : 13th shock
else if (t > DA2temp12+100 && t < DA2temp13) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp12+100)))}
else if (t >= DA2temp13 && t <= DA2temp13+100) {DA2=DA_S } : 14th shock
else if (t > DA2temp13+100 && t < DA2temp14) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp13+100)))}
else if (t >= DA2temp14 && t <= DA2temp14+100) {DA2=DA_S} : 15th shock
else if (t > DA2temp14+100 && t < DA2temp15) {DA2 = 1.0 + (DA_S-1)*exp(-Beta2*(t-(DA2temp14+100)))}
else if (t >= DA2temp15 && t <= DA2temp15+100) {DA2=DA_S} : 16th shock
else { DA2 = 1.0}
}
FUNCTION NEn(NEstart1 (ms), NEstop1 (ms)) {
LOCAL NEtemp1, NEtemp2, NEtemp3, NEtemp4, NEtemp5, NEtemp6, NEtemp7, NEtemp8, NEtemp9, NEtemp10, NEtemp11, NEtemp12, NEtemp13, NEtemp14, NEtemp15, NEtemp16, NEtemp17, NEtemp18, NEtemp19, NEtemp20, NEtemp21, NEtemp22, NEtemp23, NEtemp24, NEtemp25, NEtemp26, NEtemp27, NEtemp28, NEtemp29, NEtemp30, NEtemp31, NEtemp32, NEtemp33, NEtemp34,s
NEtemp1 = NEstart1+4000
NEtemp2 = NEtemp1+4000
NEtemp3 = NEtemp2+4000
NEtemp4 = NEtemp3+4000
NEtemp5 = NEtemp4+4000
NEtemp6 = NEtemp5+4000
NEtemp7 = NEtemp6+4000
NEtemp8 = NEtemp7+4000
NEtemp9 = NEtemp8+4000
NEtemp10 = NEtemp9+4000
NEtemp11 = NEtemp10+4000
NEtemp12 = NEtemp11+4000
NEtemp13 = NEtemp12+4000
NEtemp14 = NEtemp13+4000
NEtemp15 = NEtemp14 + 4000 + 100000 : 100sec Gap
NEtemp16 = NEtemp15 + 4000
NEtemp17 = NEtemp16 + 4000
NEtemp18 = NEtemp17 + 4000
NEtemp19 = NEtemp18 + 4000
NEtemp20 = NEtemp19 + 4000
NEtemp21 = NEtemp20 + 4000
NEtemp22 = NEtemp21 + 4000
NEtemp23 = NEtemp22 + 4000
NEtemp24 = NEtemp23 + 4000
NEtemp25 = NEtemp24 + 4000
NEtemp26 = NEtemp25 + 4000
NEtemp27 = NEtemp26 + 4000
NEtemp28 = NEtemp27 + 4000
NEtemp29 = NEtemp28 + 4000
NEtemp30 = NEtemp29 + 4000
NEtemp31 = NEtemp30 + 4000
NEtemp32 = NEtemp31 + 4000
NEtemp33 = NEtemp32 + 4000
NEtemp34 = NEtemp33 + 4000
if (t <= NEstart1) { NEn = 1.0}
else if (t >= NEstart1 && t <= NEstop1) {NEn = NE_t1} : 2nd tone in early conditioning (EC)
else if (t > NEstop1 && t < NEtemp1) {NEn = 1.0 + (NE_t1-1)*exp(-Beta1*(t-NEstop1))} : Basal level
else if (t >= NEtemp1 && t <= NEtemp1+500) {NEn = NE_t1} : 3rd tone EC
else if (t > NEtemp1+500 && t < NEtemp2) {NEn = 1.0 + (NE_t1-1)*exp(-Beta1*(t-NEstop1))} : Basal level
else if (t >= NEtemp2 && t <= NEtemp2+500) {NEn = NE_t1} : 4th tone EC
else if (t > NEtemp2+500 && t < NEtemp3) {NEn = 1.0 + (NE_t1-1)*exp(-Beta1*(t-(NEtemp2+500)))} : Basal level
else if (t >= NEtemp3 && t <= NEtemp3+500) {NEn = NE_t1} : 5th tone EC
else if (t > NEtemp3+500 && t < NEtemp4) {NEn = 1.0 + (NE_t1-1)*exp(-Beta1*(t-(NEtemp3+500)))} : Basal level
else if (t >= NEtemp4 && t <= NEtemp4+500) {NEn = NE_t1} : 6th tone EC
else if (t > NEtemp4+500 && t < NEtemp5) {NEn = 1.0 + (NE_t1-1)*exp(-Beta1*(t-(NEtemp4+500)))} : Basal level
else if (t >= NEtemp5 && t <= NEtemp5+500) {NEn = NE_t1} : 7th tone EC
else if (t > NEtemp5+500 && t < NEtemp6) {NEn = 1.0 + (NE_t1-1)*exp(-Beta1*(t-(NEtemp5+500)))} : Basal level
else if (t >= NEtemp6 && t <= NEtemp6+500) {NEn = NE_t1} : 8th tone EC
else if (t > NEtemp6+500 && t < NEtemp7) {NEn = 1.0 + (NE_t1-1)*exp(-Beta1*(t-(NEtemp6+500)))} : Basal level
else if (t >= NEtemp7 && t <= NEtemp7+500) {NEn = NE_t2} : 9th tone - Second Step late cond (LC)
else if (t > NEtemp7+500 && t < NEtemp8) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp7+500)))} : Basal level
else if (t >= NEtemp8 && t <= NEtemp8+500) {NEn = NE_t2} : 10th tone LC
else if (t > NEtemp8+500 && t < NEtemp9) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp8+500)))} : Basal level
else if (t >= NEtemp9 && t <= NEtemp9+500) {NEn = NE_t2} : 11th tone LC
else if (t > NEtemp9+500 && t < NEtemp10) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp9+500)))} : Basal level
else if (t >= NEtemp10 && t <= NEtemp10+500) {NEn = NE_t2} : 12th tone LC
else if (t > NEtemp10+500 && t < NEtemp11) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp10+500)))} : Basal level
else if (t >= NEtemp11 && t <= NEtemp11+500) {NEn = NE_t2} : 13th tone LC
else if (t > NEtemp11+500 && t < NEtemp12) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp11+500)))} : Basal level
else if (t >= NEtemp12 && t <= NEtemp12+500) {NEn = NE_t2} : 14th tone LC
else if (t > NEtemp12+500 && t < NEtemp13) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp12+500)))} : Basal level
else if (t >= NEtemp13 && t <= NEtemp13+500) {NEn = NE_t2} : 15th tone LC
else if (t > NEtemp13+500 && t < NEtemp14) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp13+500)))} : Basal level
else if (t >= NEtemp14 && t <= NEtemp14+500) {NEn = NE_t2} : 16th tone LC
else if (t > NEtemp14+500 && t < NEtemp15) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp14+500)))} : Basal level
else if (t >= NEtemp15 && t <= NEtemp15+500) {NEn = NE_t2} : 1st tone EE
else if (t > NEtemp15+500 && t < NEtemp16) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp15+500)))} : Basal level
else if (t >= NEtemp16 && t <= NEtemp16+500) {NEn = NE_t2} : 2nd tone EE
else if (t > NEtemp16+500 && t < NEtemp17) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp16+500)))} : Basal level
else if (t >= NEtemp17 && t <= NEtemp17+500) {NEn = NE_t2} : 3rd tone EE
else if (t > NEtemp17+500 && t < NEtemp18) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp17+500)))} : Basal level
else if (t >= NEtemp18 && t <= NEtemp18+500) {NEn = NE_t2} : 4th tone EE
else if (t > NEtemp18+500 && t < NEtemp19) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp18+500)))} : Basal level
else if (t >= NEtemp19 && t <= NEtemp19+500) {NEn = NE_t3} : 5th tone EE
else if (t > NEtemp19+500 && t < NEtemp20) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp19+500)))} : Basal level
else if (t >= NEtemp20 && t <= NEtemp20+500) {NEn = NE_t3} : 6th tone EE
else if (t > NEtemp20+500 && t < NEtemp21) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp20+500)))} : Basal level
else if (t >= NEtemp21 && t <= NEtemp21+500) {NEn = NE_t3} : 7th tone EE
else if (t > NEtemp21+500 && t < NEtemp22) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp21+500)))} : Basal level
else if (t >= NEtemp22 && t <= NEtemp22+500) {NEn = NE_t3} : 8th tone EE
else if (t > NEtemp22+500 && t < NEtemp23) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp22+500)))} : Basal level
else if (t >= NEtemp23 && t <= NEtemp23+500) {NEn = NE_t3} : 9th tone EE
else if (t > NEtemp23+500 && t < NEtemp24) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp23+500)))} : Basal level
else if (t >= NEtemp24 && t <= NEtemp24+500) {NEn = NE_t3} : 10th tone EE
else if (t > NEtemp24+500 && t < NEtemp25) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp24+500)))} : Basal level
else if (t >= NEtemp25 && t <= NEtemp25+500) {NEn = NE_t3} : 11th tone EE
else if (t > NEtemp25+500 && t < NEtemp26) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp25+500)))} : Basal level
else if (t >= NEtemp26 && t <= NEtemp26+500) {NEn = NE_t3} : 12th tone EE
else if (t > NEtemp26+500 && t < NEtemp27) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp26+500)))} : Basal level
else if (t >= NEtemp27 && t <= NEtemp27+500) {NEn = NE_t3} : 13th tone EE
else if (t > NEtemp27+500 && t < NEtemp28) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp27+500)))} : Basal level
else if (t >= NEtemp28 && t <= NEtemp28+500) {NEn = NE_t3} : 14th tone EE
else if (t > NEtemp28+500 && t < NEtemp29) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp28+500)))} : Basal level
else if (t >= NEtemp29 && t <= NEtemp29+500) {NEn = NE_t3} : 15th tone EE
else if (t > NEtemp29+500 && t < NEtemp30) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp29+500)))} : Basal level
else if (t >= NEtemp30 && t <= NEtemp30+500) {NEn = NE_t3} : 16th tone EE
else if (t > NEtemp30+500 && t < NEtemp31) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp30+500)))} : Basal level
else if (t >= NEtemp31 && t <= NEtemp31+500) {NEn = NE_t3} : 17th tone EE
else if (t > NEtemp31+500 && t < NEtemp32) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp31+500)))} : Basal level
else if (t >= NEtemp32 && t <= NEtemp32+500) {NEn = NE_t3} : 18th tone EE
else if (t > NEtemp32+500 && t < NEtemp33) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp32+500)))} : Basal level
else if (t >= NEtemp33 && t <= NEtemp33+500) {NEn = NE_t3} : 19th tone EE
else if (t > NEtemp33+500 && t < NEtemp34) {NEn = 1.0 + (NE_t2-1)*exp(-Beta2*(t-(NEtemp33+500)))} : Basal level
else if (t >= NEtemp34 && t <= NEtemp34+500) {NEn = NE_t3} : 20th tone EE
else { NEn = 1.0}
}
FUNCTION NE2(NEstart2 (ms), NEstop2 (ms)) {
LOCAL NE2temp1, NE2temp2, NE2temp3, NE2temp4, NE2temp5, NE2temp6, NE2temp7, NE2temp8, NE2temp9, NE2temp10, NE2temp11, NE2temp12, NE2temp13, NE2temp14, NE2temp15, NE2temp16,s
NE2temp1 = NEstart2 + 4000
NE2temp2 = NE2temp1 + 4000
NE2temp3 = NE2temp2 + 4000
NE2temp4 = NE2temp3 + 4000
NE2temp5 = NE2temp4 + 4000
NE2temp6 = NE2temp5 + 4000
NE2temp7 = NE2temp6 + 4000
NE2temp8 = NE2temp7 + 4000
NE2temp9 = NE2temp8 + 4000
NE2temp10 = NE2temp9 + 4000
NE2temp11 = NE2temp10 + 4000
NE2temp12 = NE2temp11 + 4000
NE2temp13 = NE2temp12 + 4000
NE2temp14 = NE2temp13 + 4000
NE2temp15 = NE2temp14 + 4000
if (t <= NEstart2) { NE2 = 1.0}
else if (t >= NEstart2 && t <= NEstop2) {NE2 = NE_S } : 1st shock
else if (t > NEstop2 && t < NE2temp1) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NEstop2+500)))}
else if (t >= NE2temp1 && t <= NE2temp1+100) {NE2=NE_S} : 2nd shock
else if (t > NE2temp1+100 && t < NE2temp2) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp1+100)))}
else if (t >= NE2temp2 && t <= NE2temp2+100) {NE2=NE_S} : 3rd shock
else if (t > NE2temp2+100 && t < NE2temp3) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp2+100)))}
else if (t >= NE2temp3 && t <= NE2temp3+100) {NE2=NE_S} : 4th shock
else if (t > NE2temp3+100 && t < NE2temp4) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp3+100)))}
else if (t >= NE2temp4 && t <= NE2temp4+100) {NE2=NE_S} : 5th shock
else if (t > NE2temp4+100 && t < NE2temp5) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp4+100)))}
else if (t >= NE2temp5 && t <= NE2temp5+100) {NE2=NE_S} : 6th shock
else if (t > NE2temp5+100 && t < NE2temp6) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp5+100)))}
else if (t >= NE2temp6 && t <= NE2temp6+100) {NE2=NE_S} : 7th shock
else if (t > NE2temp6+100 && t < NE2temp7) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp6+100)))}
else if (t >= NE2temp7 && t <= NE2temp7+100) {NE2=NE_S} : 8th shock
else if (t > NE2temp7+100 && t < NE2temp8) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp7+100)))}
else if (t >= NE2temp8 && t <= NE2temp8+100) {NE2=NE_S } : 9th shock
else if (t > NE2temp8+100 && t < NE2temp9) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp8+100)))}
else if (t >= NE2temp9 && t <= NE2temp9+100) {NE2=NE_S } : 10th shock
else if (t > NE2temp9+100 && t < NE2temp10) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp9+100)))}
else if (t >= NE2temp10 && t <= NE2temp10+100) {NE2=NE_S} : 11th shock
else if (t > NE2temp10+100 && t < NE2temp11) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp10+100)))}
else if (t >= NE2temp11 && t <= NE2temp11+100) {NE2=NE_S } : 12th shock
else if (t > NE2temp11+100 && t < NE2temp12) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp11+100)))}
else if (t >= NE2temp12 && t <= NE2temp12+100) {NE2=NE_S} : 13th shock
else if (t > NE2temp12+100 && t < NE2temp13) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp12+100)))}
else if (t >= NE2temp13 && t <= NE2temp13+100) {NE2=NE_S } : 14th shock
else if (t > NE2temp13+100 && t < NE2temp14) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp13+100)))}
else if (t >= NE2temp14 && t <= NE2temp14+100) {NE2=NE_S} : 15th shock
else if (t > NE2temp14+100 && t < NE2temp15) {NE2 = 1.0 + (NE_S-1)*exp(-Beta2*(t-(NE2temp14+100)))}
else if (t >= NE2temp15 && t <= NE2temp15+100) {NE2=NE_S} : 16th shock
else { NE2 = 1.0}
}
FUNCTION GAP1(GAPstart1 (ms), GAPstop1 (ms)) {
LOCAL s
if (t <= GAPstart1) { GAP1 = 1}
else if (t >= GAPstop1 ) {GAP1 = 1} : During the Gap, apply lamda2*2
else { GAP1 = 1}
}
FUNCTION unirand() { : uniform random numbers between 0 and 1
unirand = scop_random()
}