<?xml version="1.0" encoding="utf-8"?> <ChannelProject> <FileVersion>1.0</FileVersion> <ExperimentList> <List> <ExperimentBase d4p1:type="ExperimentIvsT" xmlns:d4p1="http://www.w3.org/2001/XMLSchema-instance"> <Name>Current Family</Name> <Temperature>20</Temperature> <ExperimentType>CurrentVsTime</ExperimentType> <Sweeps>15</Sweeps> <Time_Sweep_ms>600</Time_Sweep_ms> <SampleInterval_us>100</SampleInterval_us> <SampleType>NSamples</SampleType> <SamplesSweep>1001</SamplesSweep> <InitialProbabilities> <InitialSweepState>SteadyState</InitialSweepState> <IntervalBetweenSweeps>1000</IntervalBetweenSweeps> <SchemeCollection> <SchemeInitialStatesProbabilities> <GateName>Scheme</GateName> <InitialProbabilities> <InitialStateProbability> <StateName>C0</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C1</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C2</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C3</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C4</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>O</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>I</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> </InitialProbabilities> </SchemeInitialStatesProbabilities> </SchemeCollection> </InitialProbabilities> <Variables> <VariableList> <VariableAndProtocol> <Name>Voltage</Name> <Value>-70</Value> <Units>mV</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>-70</First> <Last>-70</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>50</StartTime> <DeltaTime>0</DeltaTime> </Step> <Step> <Type>Step</Type> <First>-40</First> <Last>-40</Last> <DeltaFirst>10</DeltaFirst> <DeltaLast>10</DeltaLast> <StartTime>500</StartTime> <DeltaTime>0</DeltaTime> </Step> <Step> <Type>Step</Type> <First>-70</First> <Last>-70</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>50</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> <VariableAndProtocol> <Name>External-K</Name> <Value>5</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>5</First> <Last>5</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>1000</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> <VariableAndProtocol> <Name>Internal-K</Name> <Value>100</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>100</First> <Last>100</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>1000</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> </VariableList> </Variables> <SimulationMethod>QMatrix</SimulationMethod> <NChannels>1000</NChannels> <SimulationType>IonicCurrent</SimulationType> <AddNoise>false</AddNoise> <NoiseStandardDeviation>0.1</NoiseStandardDeviation> </ExperimentBase> <ExperimentBase d4p1:type="ExperimentIvsT" xmlns:d4p1="http://www.w3.org/2001/XMLSchema-instance"> <Name>DP_inact</Name> <Temperature>20</Temperature> <ExperimentType>CurrentVsTime</ExperimentType> <Sweeps>400</Sweeps> <Time_Sweep_ms>400</Time_Sweep_ms> <SampleInterval_us>100</SampleInterval_us> <SampleType>NSamples</SampleType> <SamplesSweep>1001</SamplesSweep> <InitialProbabilities> <InitialSweepState>SteadyState</InitialSweepState> <IntervalBetweenSweeps>1000</IntervalBetweenSweeps> <SchemeCollection> <SchemeInitialStatesProbabilities> <GateName>Scheme</GateName> <InitialProbabilities> <InitialStateProbability> <StateName>C0</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C1</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C2</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C3</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C4</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>O</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>I</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> </InitialProbabilities> </SchemeInitialStatesProbabilities> </SchemeCollection> </InitialProbabilities> <Variables> <VariableList> <VariableAndProtocol> <Name>Voltage</Name> <Value>-100</Value> <Units>mV</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>-100</First> <Last>-100</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>50</StartTime> <DeltaTime>0</DeltaTime> </Step> <Step> <Type>Step</Type> <First>0</First> <Last>0</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>0</StartTime> <DeltaTime>0.1</DeltaTime> </Step> <Step> <Type>Step</Type> <First>-100</First> <Last>-100</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>5</StartTime> <DeltaTime>0</DeltaTime> </Step> <Step> <Type>Step</Type> <First>70</First> <Last>70</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>50</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> <VariableAndProtocol> <Name>External-K</Name> <Value>5</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>5</First> <Last>5</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>1000</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> <VariableAndProtocol> <Name>Internal-K</Name> <Value>100</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>100</First> <Last>100</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>1000</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> </VariableList> </Variables> <SimulationMethod>QMatrix</SimulationMethod> <NChannels>1000</NChannels> <SimulationType>IonicCurrent</SimulationType> <AddNoise>false</AddNoise> <NoiseStandardDeviation>0.1</NoiseStandardDeviation> </ExperimentBase> <ExperimentBase d4p1:type="ExperimentIvsT" xmlns:d4p1="http://www.w3.org/2001/XMLSchema-instance"> <Name>SP_inact</Name> <Temperature>20</Temperature> <ExperimentType>CurrentVsTime</ExperimentType> <Sweeps>1</Sweeps> <Time_Sweep_ms>600</Time_Sweep_ms> <SampleInterval_us>100</SampleInterval_us> <SampleType>NSamples</SampleType> <SamplesSweep>5000</SamplesSweep> <InitialProbabilities> <InitialSweepState>SteadyState</InitialSweepState> <IntervalBetweenSweeps>1000</IntervalBetweenSweeps> <SchemeCollection> <SchemeInitialStatesProbabilities> <GateName>Scheme</GateName> <InitialProbabilities> <InitialStateProbability> <StateName>C0</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C1</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C2</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C3</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>C4</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>O</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> <InitialStateProbability> <StateName>I</StateName> <Probability>0.142857149</Probability> <RelativeProbability>0</RelativeProbability> </InitialStateProbability> </InitialProbabilities> </SchemeInitialStatesProbabilities> </SchemeCollection> </InitialProbabilities> <Variables> <VariableList> <VariableAndProtocol> <Name>Voltage</Name> <Value>-70</Value> <Units>mV</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>-70</First> <Last>-70</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>50</StartTime> <DeltaTime>0</DeltaTime> </Step> <Step> <Type>Step</Type> <First>0</First> <Last>0</Last> <DeltaFirst>10</DeltaFirst> <DeltaLast>10</DeltaLast> <StartTime>500</StartTime> <DeltaTime>0</DeltaTime> </Step> <Step> <Type>Step</Type> <First>-70</First> <Last>-70</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>50</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> <VariableAndProtocol> <Name>External-K</Name> <Value>5</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>5</First> <Last>5</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>1000</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> <VariableAndProtocol> <Name>Internal-K</Name> <Value>120</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> <Steps> <Step> <Type>Step</Type> <First>120</First> <Last>120</Last> <DeltaFirst>0</DeltaFirst> <DeltaLast>0</DeltaLast> <StartTime>1000</StartTime> <DeltaTime>0</DeltaTime> </Step> </Steps> </VariableAndProtocol> </VariableList> </Variables> <SimulationMethod>QMatrix</SimulationMethod> <NChannels>1000</NChannels> <SimulationType>IonicCurrent</SimulationType> <AddNoise>false</AddNoise> <NoiseStandardDeviation>0.1</NoiseStandardDeviation> </ExperimentBase> <ExperimentBase d4p1:type="ExperimentExpressionVsX" xmlns:d4p1="http://www.w3.org/2001/XMLSchema-instance"> <Name>SSI</Name> <Temperature>20</Temperature> <ExperimentType>SteadyStateExpressionVsX</ExperimentType> <Variables> <VariableList> <VariableAndDelta> <Name>Voltage</Name> <Value>0</Value> <Units>mV</Units> <DeltaValue>0</DeltaValue> </VariableAndDelta> <VariableAndDelta> <Name>External-K</Name> <Value>5</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> </VariableAndDelta> <VariableAndDelta> <Name>Internal-K</Name> <Value>100</Value> <Units>mM</Units> <DeltaValue>0</DeltaValue> </VariableAndDelta> </VariableList> </Variables> <Sweeps>1</Sweeps> <SamplesSweep>300</SamplesSweep> <SelectedVariable>Voltage</SelectedVariable> <StartValue>-100</StartValue> <FinalValue>100</FinalValue> <Expression>C0+C1+C2+C3+C4+O</Expression> </ExperimentBase> </List> </ExperimentList> <IndexOfSelectedExperiment>1</IndexOfSelectedExperiment> <Description> <string /> <string>Beck, E.J., M. Bowlby, W.F. An, K.J. Rhodes, and M. Covarrubias. 2002.</string> <string> Remodeling inactivation gating of Kv4 channels by KChIP-1, a small-molecular-weight calcium binding protein. J. Physiol. 538:691-706. </string> </Description> <Channel d2p1:type="ChannelScheme" xmlns:d2p1="http://www.w3.org/2001/XMLSchema-instance"> <Name>Channel</Name> <IonicCurrentList> <IonicCurrentBase d2p1:type="OhmicCurrent"> <Ion> <Name>K</Name> <Type>Ion</Type> <Valence>1</Valence> </Ion> <Conductance>10</Conductance> </IonicCurrentBase> </IonicCurrentList> <Scheme> <Name>Scheme</Name> <SchemeID>406018</SchemeID> <LayersToShow> <LayerA>0</LayerA> <LayerB>0</LayerB> </LayersToShow> <TransitionCollection> <TransitionView d2p1:type="TransitionViewAB"> <Transition d2p1:type="TransitionAB"> <StateA>C0</StateA> <StateB>C1</StateB> <Activated>true</Activated> <RateA>4*a</RateA> <RateB>b</RateB> </Transition> </TransitionView> <TransitionView d2p1:type="TransitionViewAB"> <Transition d2p1:type="TransitionAB"> <StateA>C1</StateA> <StateB>C2</StateB> <Activated>true</Activated> <RateA>3*a</RateA> <RateB>2*b</RateB> </Transition> </TransitionView> <TransitionView d2p1:type="TransitionViewAB"> <Transition d2p1:type="TransitionAB"> <StateA>C2</StateA> <StateB>C3</StateB> <Activated>true</Activated> <RateA>2*a</RateA> <RateB>3*b</RateB> </Transition> </TransitionView> <TransitionView d2p1:type="TransitionViewAB"> <Transition d2p1:type="TransitionAB"> <StateA>C3</StateA> <StateB>C4</StateB> <Activated>true</Activated> <RateA>a</RateA> <RateB>4*b</RateB> </Transition> </TransitionView> <TransitionView d2p1:type="TransitionViewAB"> <Transition d2p1:type="TransitionAB"> <StateA>C4</StateA> <StateB>O</StateB> <Activated>true</Activated> <RateA>kco</RateA> <RateB>koc</RateB> </Transition> </TransitionView> <TransitionView d2p1:type="TransitionViewAB"> <Transition d2p1:type="TransitionAB"> <StateA>O</StateA> <StateB>I</StateB> <Activated>true</Activated> <RateA>koi</RateA> <RateB>kio</RateB> </Transition> </TransitionView> </TransitionCollection> <SchemeFunctionsList> <FunctionBase d2p1:type="ExponentialFRTFunction"> <Name>a</Name> <Type>ExponentialFRT</Type> <NumberParameters>2</NumberParameters> <P> <double>3351.95557</double> <double>0.06336615</double> </P> </FunctionBase> <FunctionBase d2p1:type="ExponentialFRTFunction"> <Name>b</Name> <Type>ExponentialFRT</Type> <NumberParameters>2</NumberParameters> <P> <double>3230.19482</double> <double>-0.7965966</double> </P> </FunctionBase> <FunctionBase d2p1:type="ExponentialFRTFunction"> <Name>kco</Name> <Type>ExponentialFRT</Type> <NumberParameters>2</NumberParameters> <P> <double>434.302551</double> <double>0.5199188</double> </P> </FunctionBase> <FunctionBase d2p1:type="ExponentialFRTFunction"> <Name>koc</Name> <Type>ExponentialFRT</Type> <NumberParameters>2</NumberParameters> <P> <double>70.37514</double> <double>-0.374497831</double> </P> </FunctionBase> <FunctionBase d2p1:type="ConstantFunction"> <Name>koi</Name> <Type>Constant</Type> <NumberParameters>1</NumberParameters> <P> <double>55.0211449</double> </P> </FunctionBase> <FunctionBase d2p1:type="ConstantFunction"> <Name>kio</Name> <Type>Constant</Type> <NumberParameters>1</NumberParameters> <P> <double>0.7999873</double> </P> </FunctionBase> </SchemeFunctionsList> <StatesList> <State> <Name>C0</Name> <StateID>155192</StateID> <Activated>true</Activated> <Charge>0</Charge> <color>SteelBlue</color> <Type>Closed</Type> <GatingValue>0</GatingValue> <Position> <X>50</X> <Y>40</Y> </Position> <ZOrder>0</ZOrder> </State> <State> <Name>C1</Name> <StateID>513997</StateID> <Activated>true</Activated> <Charge>0.85996275</Charge> <color>SteelBlue</color> <Type>Closed</Type> <GatingValue>0</GatingValue> <Position> <X>180</X> <Y>40</Y> </Position> <ZOrder>0</ZOrder> </State> <State> <Name>C2</Name> <StateID>406018</StateID> <Activated>true</Activated> <Charge>1.7199255</Charge> <color>SteelBlue</color> <Type>Closed</Type> <GatingValue>0</GatingValue> <Position> <X>300</X> <Y>40</Y> </Position> <ZOrder>0</ZOrder> </State> <State> <Name>C3</Name> <StateID>9741</StateID> <Activated>true</Activated> <Charge>2.5798882499999998</Charge> <color>SteelBlue</color> <Type>Closed</Type> <GatingValue>0</GatingValue> <Position> <X>420</X> <Y>40</Y> </Position> <ZOrder>0</ZOrder> </State> <State> <Name>C4</Name> <StateID>869635</StateID> <Activated>true</Activated> <Charge>3.439851</Charge> <color>SteelBlue</color> <Type>Closed</Type> <GatingValue>0</GatingValue> <Position> <X>540</X> <Y>40</Y> </Position> <ZOrder>0</ZOrder> </State> <State> <Name>O</Name> <StateID>562829</StateID> <Activated>true</Activated> <Charge>4.334267631</Charge> <color>SteelBlue</color> <Type>Open</Type> <GatingValue>1.96</GatingValue> <Position> <X>660</X> <Y>40</Y> </Position> <ZOrder>0</ZOrder> </State> <State> <Name>I</Name> <StateID>707462</StateID> <Activated>true</Activated> <Charge>4.334267631</Charge> <color>SteelBlue</color> <Type>Closed</Type> <GatingValue>0</GatingValue> <Position> <X>770</X> <Y>40</Y> </Position> <ZOrder>0</ZOrder> </State> </StatesList> <NGates>1</NGates> </Scheme> </Channel> </ChannelProject>