IonChannelLab is available at: http://www.jadesantiago.com/Electrophysiology/IonChannelLab/Download/ For more information on the program see De Santiago Castillo et al. Channels (2010). The .ichl files contain the schemes, parameters and simulations used. The interface contains two areas: 1)Kinetic modeling - schemes and parameters 2)Simulations - where experiments are run There may be some need to change sampling intervals or number of sweeps in the double pulse protocols to get the full resolution. Simulations may be saved as a .txt file by clicking the save button and selecting simulation. To recreate figure 2, there will be a need to save each SSI and current family before switching the lambda1 (kio) value and repeating. Any questions can be directed to david.ritter@jefferson.edu