//genesis float dt = 20e-6 str disk = "results_gap/" int amp = {1 / dt} str a = (amp) int i, k, l str el, name float t /********************************************************************* ** Simple Granule cell model script (#1) ** Carl Piaf BBF 1994 *********************************************************************/ str filename = (disk) @ "test2" /* always include these default definitions! */ include defaults str cellpath = "/Granule" /* Purkinje cell constants */ include Gran_const.g /* special scripts to create the prototypes */ include Gran_chan_tab.g //include Gran_synchan2_biexpGABA.g include Gran_synchan.g include Gran_comp.g /* Set the clocks */ for (i = 0; i <= 7; i = i + 1) setclock {i} {dt} end setclock 8 2.0e-5 setclock 9 1 /* To ensure that all subsequent elements are made in the library */ if (! {exists /library/granule}) create neutral /library/granule end ce /library/granule /* These make the prototypes of channels and compartments that can be ** invoked in .p files */ make_Granule_chans make_Granule_syns make_Granule_comps /* call Gran_InNa TABSAVE tabInNa37.data call Gran_KDr TABSAVE tabKDr37.data call Gran_KA TABSAVE tabKA37.data call Gran_CaHVA TABSAVE tabCaHVA37.data call Gran_H TABSAVE tabH37.data call Moczyd_KC TABSAVE tabKCa37.data */ //make_Vmgraph /* create the model and set up the run cell mode */ // read cell data from .p file readcell Gran1M0.p {cellpath} // Gran1M0.p {cellpath} createmap {cellpath} /granule_cell_layer \ 2 1 -delta 1.0 0.0 -origin 0.0 0.0 disable {cellpath} create diffamp /granule_cell_layer/Granule[1]/diffamp setfield /granule_cell_layer/Granule[1]/diffamp gain 1e-9 saturation 10e8 addmsg /granule_cell_layer/Granule[1]/soma /granule_cell_layer/Granule[1]/diffamp PLUS Vm addmsg /granule_cell_layer/Granule[0]/soma /granule_cell_layer/Granule[1]/diffamp MINUS Vm addmsg /granule_cell_layer/Granule[1]/diffamp /granule_cell_layer/Granule[0]/soma INJECT output create neutral /library/granule/soma/mf_presyn disable /library/granule/soma/mf_presyn setfield /library/granule/soma/mf_presyn z 0 // Comment out whichever one to switch it off //addmsg /library/granule/soma/mf_presyn /Granule/soma/GABAA ACTIVATION z //addmsg /library/granule/soma/mf_presyn /Granule/soma/GABAB ACTIVATION z //addmsg /library/granule/soma/mf_presyn /Granule/soma/mf_NMDA ACTIVATION z //addmsg /library/granule/soma/mf_presyn /Granule/soma/mf_AMPA ACTIVATION z /* Create the output element */ create asc_file /output/plot_out //create disk_out /output/plot_out useclock /output/plot_out 8 enable /output enable /output/plot_out ce {cellpath} // setup the hines solver echo preparing hines solver... create hsolve /granule_cell_layer/Granule[0]/solve ce /granule_cell_layer/Granule[0]/solve setfield . path "../##[][TYPE=compartment]" comptmode 1 chanmode 4 call . SETUP create hsolve /granule_cell_layer/Granule[1]/solve ce /granule_cell_layer/Granule[1]/solve setfield . path "../##[][TYPE=compartment]" comptmode 1 chanmode 4 call . SETUP setmethod 11 reset // setmethod 0 // addmsg {cellpath}/soma /output/plot_out SAVE Vm el = ({findsolvefield /granule_cell_layer/Granule[0]/solve soma Vm}) //#1 addmsg /granule_cell_layer/Granule[0]/solve /output/plot_out SAVE {el} // el = ({findsolvefield /granule_cell_layer/Granule[1]/solve soma Vm}) //#1 // addmsg /granule_cell_layer/Granule[1]/solve /output/plot_out SAVE {el} setfield /output/plot_out filename {filename} initialize 1 leave_open 1 \ flush 1 echo Output to {filename} //check reset // Synaptic stimulation protocol /* setfield {cellpath}/soma inject 20.0e-12 call {cellpath}/solve HPUT {cellpath}/soma step 0.3 -time */ /* setfield {cellpath}/soma Em -0.065 setfield {cellpath}/soma Vm -0.0625 setfield {cellpath}/soma initVm -0.0625 call {cellpath}/solve HPUT {cellpath}/soma */ /* // step 0.05 -time setfield /library/granule/soma/mf_presyn z {amp} step 1 setfield /library/granule/soma/mf_presyn z 0 step 100e-3 -time */ step 0.3 -t // Current injection protocol step 0.1 -time setfield /granule_cell_layer/Granule[1]/soma inject 20.0e-12 call /granule_cell_layer/Granule[1]/solve HPUT /granule_cell_layer/Granule[1]/soma step 0.5 -time call /granule_cell_layer/Granule[1]/solve HSAVE /granule_cell_layer/Granule[1]/soma setfield /granule_cell_layer/Granule[1]/soma inject 0.0e-12 call /granule_cell_layer/Granule[1]/solve HPUT /granule_cell_layer/Granule[1]/soma step 0.2 -time