//genesis float dt = 20e-6 str disk = "results/" int amp = {1 / dt} str a = (amp) int i, k, l str el, name float t /********************************************************************* ** Simple Granule cell model script (#1) ** Carl Piaf BBF 1994 *********************************************************************/ str filename = (disk) @ "G_GABA_I60_w15_0.333" //str filename = (disk) @ "G_GABA0.332" /* always include these default definitions! */ include defaults str cellpath = "/Granule" /* Purkinje cell constants */ include Gran_const.g /* special scripts to create the prototypes */ include Gran_chan_tab.g //include Gran_synchan2_biexpGABA.g include Gran_synchan.g //include Gran_comp.g include Gran_comp_soma_dend.g /* Set the clocks */ for (i = 0; i <= 7; i = i + 1) setclock {i} {dt} end setclock 8 2.0e-5 setclock 9 1 /* To ensure that all subsequent elements are made in the library */ if (! {exists /library/granule}) create neutral /library/granule end ce /library/granule /* These make the prototypes of channels and compartments that can be ** invoked in .p files */ make_Granule_chans make_Granule_syns //make_Granule_comps make_Granule_comps_soma_dend /* call Gran_InNa TABSAVE tabInNa37.data call Gran_KDr TABSAVE tabKDr37.data call Gran_KA TABSAVE tabKA37.data call Gran_CaHVA TABSAVE tabCaHVA37.data call Gran_H TABSAVE tabH37.data call Moczyd_KC TABSAVE tabKCa37.data */ //make_Vmgraph setfield /library/granule/soma/GABAA \ tau1 {{getfield /library/granule/soma/GABAA tau1} / 3} \ tau2 {{getfield /library/granule/soma/GABAA tau2} / 3} \ gmax {{getfield /library/granule/soma/GABAA gmax} * 15} //setfield /library/granule/soma/InNa Gbar {{getfield /library/granule/soma/InNa Gbar} * 5.0} //setfield /library/granule/soma/KDr Gbar {{getfield /library/granule/soma/KDr Gbar} * 5.0} /* create the model and set up the run cell mode */ // read cell data from .p file readcell Gran1M0_dend.p {cellpath} // Gran1M0.p {cellpath} setfield /Granule/soma/InNa Gbar {{getfield /Granule/soma/InNa Gbar} * 6.0} setfield /Granule/soma/KDr Gbar {{getfield /Granule/soma/KDr Gbar} * 6.0} create neutral /library/granule/soma/mf_presyn disable /library/granule/soma/mf_presyn setfield /library/granule/soma/mf_presyn z 0 // Comment out whichever one to switch it off addmsg /library/granule/soma/mf_presyn /Granule/soma/GABAA ACTIVATION z //addmsg /library/granule/soma/mf_presyn /Granule/soma/GABAB ACTIVATION z //addmsg /library/granule/soma/mf_presyn /Granule/soma/mf_NMDA ACTIVATION z //addmsg /library/granule/soma/mf_presyn /Granule/soma/mf_AMPA ACTIVATION z /* Create the output element */ create asc_file /output/plot_out useclock /output/plot_out 8 enable /output enable /output/plot_out ce {cellpath} // setup the hines solver echo preparing hines solver... create hsolve solve ce solve // if this is set then reset will NOT change Vm in Hines setfield . path "../##[][TYPE=compartment]" comptmode 1 chanmode 4 call . SETUP setmethod 11 // addmsg {cellpath}/soma /output/plot_out SAVE Vm el = ({findsolvefield {cellpath}/solve soma Vm}) //#4 addmsg {cellpath}/solve /output/plot_out SAVE {el} /* el = ({findsolvefield {cellpath}/solve soma/GABAA Gk}) addmsg {cellpath}/solve /output/plot_out SAVE {el} // el = ({findsolvefield {cellpath}/solve soma/GABAA Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} // */ setfield /output/plot_out filename {filename} initialize 1 leave_open 1 \ flush 1 echo Output to {filename} //check reset // Synaptic stimulation protocol setfield {cellpath}/soma inject 60.0e-12 call {cellpath}/solve HPUT {cellpath}/soma step 0.333 -time setfield /library/granule/soma/mf_presyn z {amp} step 1 setfield /library/granule/soma/mf_presyn z 0 step 100e-3 -time //step 0.3 -t exit