: test units: modlunit ostim.mod
TITLE Optical Stimulation
NEURON {
POINT_PROCESS ostim
RANGE radius
RANGE delay, amp, dur
RANGE pulses, isi, pcount
POINTER irradiance
POINTER flux
POINTER photons
POINTER tstimon
POINTER tstimoff
RANGE wavelength
}
UNITS {
(mW) = (milliwatt)
(W) = (watt)
PI = (pi) (1)
}
PARAMETER {
radius = 100 (um)
delay = 1 (ms)
dur = 5 (ms)
: Aravanis et al 2007
: Psrc = 20 mW
: Af = 0.6
: r = 0.1 mm
: Irradiance = Af * Psrc/(pi * r**2) = 380 mW/mm2 --> 0.38 W/mm2 --> 38 W/cm2
amp = 38 (W/cm2)
wavelength = 4.73e-7 (m)
h = 6.6260693e-34 (m2 kg/s) : planck's constant
c = 299792458.0 (m/s) : speed of light
: Number of pulses
pulses = 1
isi = 1 (ms) : Inter-spike interval
}
ASSIGNED {
on
irradiance (W/cm2) : W/cm2
flux (1/ms cm2) : photons/ms cm2
photons (photons/ms) : photons/ms
pcount
tstimon
tstimoff
}
INITIAL {
on = 0
pcount = pulses
irradiance = 0
flux = 0
photons = 0
net_send(delay,1) : Sends a packet that should arrive after given initial_delay
tstimon = 0
tstimoff = 0
}
BEFORE BREAKPOINT {
if (on==0) {
irradiance = 0
photons = 0
flux = 0
} else if (on==1) {
calc_irradiance_photons_flux()
}
}
NET_RECEIVE (w) {
if (flag==1) {
on = 1
tstimon = t
pcount = pcount - 1
if (pcount>0) {
net_send(dur,3)
} else {
net_send(dur,2)
}
}else if (flag==2) { : stimulus complete
on = 0
tstimoff = t
}else if (flag==3) { : spike train
on = 0
net_send(isi,1)
tstimoff = t
}
}
FUNCTION calc_irradiance_photons_flux() {
LOCAL photon_energy, area
irradiance = amp
photon_energy = h * c / wavelength : J / photon
flux = (1 / 1000) * amp / photon_energy : (1 s / 1000 ms) * (W/cm2) * (photons/W s)
: --> photons/ms cm2
area = PI * (radius ^ 2) : um2
photons = 1e-8 * flux * area : (1 cm2/1e8 um2) * (um2) * (photons/ms cm2)
}