//First, run nrnivmodl *.mod file to compile all the channels.
load_file("nrngui.hoc")
load_file("baseline_values.txt")
load_file("all_tau_vecs.hoc")
load_file("../Elec/Nov9IR2a_spine.hoc")
load_file("ranstream.hoc")
xopen("electro_procs.hoc")
// Using SEClamp, as recommended on the NEURON User Forum. See init_PFC.hoc for details.
INITDUR = 80
steps_per_ms = 40
dt = 0.025
load_file("basic_procs.hoc")
set_pas(G_PAS)
set_naf(G_NAF)
set_nap(G_NAP)
set_kir(G_KIR)
set_kas(G_KAS)
set_kaf(G_KAF)
set_kdr(G_KDR)
set_bkkca(G_BKKCA)
set_skkca(G_SKKCA)
set_caL(P_CAL)
set_caL13(P_CAL13)
set_can(P_CAN)
set_caq(P_CAQ)
set_car(P_CAR)
set_cat(P_CAT)
set_cainf(CAINF)
set_taur(TAUR)
set_cadrive(CA_DRIVE)
set_pump(CA_PUMP)
print "setup complete"
/**** This is HS's new codes: ***/
objref nslist, rslist, rs
nslist = new List()
rslist = new List()
objref tvec, idvec, nil
tvec = new Vector()
idvec = new Vector()
random_stream_offset_ = 500 // Adjacent streams will be correlated by this offset.
// Seeds for network architecture and stimulus trains.
connect_random_low_start_ = 1
run_random_low_start_ = 2
proc restart() { local i
for i = 0, rslist.count()-1 rslist.o(i).start()
}
print "Addsynapese good?"
distance()
objref synBranches, synLoc
/*** new parameter settings as of 23 Jan 2012 ***/
VO = -80
V0 = -80 //avoid ambiguity of "Capital O" vs "zero 0"
/****
set up a Voltage Clamp
****/
objref seClamp
soma seClamp = new SEClamp(0.5)
seClamp.dur1 = 1e9
seClamp.amp1 = -80
seClamp.dur2 = 0
/***** end voltage clamp ****/
/********************** copied from synTweak.hoc in ~/LuebkeAmatrudo_forCluster *********/
strdef synFilename
objref synFout, tv, iv
objref rs
/**********************
Written originally in main_PFC_simEPSC_all.hoc; now copied here.
$1 number of synapses
$2 value of tau1
$3 value of tau2
$4 value of gAMPA
$s5 file basename
**********************/
proc synTweak() { local i, vecsz, btyp
adjust_tau1($2,$1)
adjust_tau2($3,$1)
adjust_gAMPA($4,$1)
sprint(synFilename,"%s_tR%.4f_tF%.4f_gAMP%.7f.Ibin",$s5,$2,$3,$4)
synFout = new File()
synFout.wopen(synFilename)
tv = new Vector()
tv.record(&t)
iv = new Vector()
iv.record(&seClamp.i)
init()
run()
vecsz = tv.size()
synFout.vwrite(&vecsz)
tv.fwrite(synFout)
iv.fwrite(synFout)
synFout.close()
sprint(synFilename,"%s_tR%.4f_tF%.4f_gAMP%.7f_dist.txt",$s5,$2,$3,$4)
synFout = new File()
synFout.wopen(synFilename)
i = 0
forsec synBranches {
btyp = 0
ifsec proximal { btyp = 1 }
ifsec middend { btyp = 2 }
ifsec distal {btyp = 3}
synFout.printf("%d\t%g\t%g\t%d\n",i,distance(synLoc.x[i]),distance(synLoc.x[i])-soma.diam,btyp)
i+=1
}
synFout.close()
}
/********************** end from synTweak.hoc in ~/LuebkeAmatrudo_forCluster *********/
xopen("plot_seClamp_i.ses")
xopen("PFC-V1_AddSynapses_neg.hoc")
nBr = 0
//WTD1
AddExcSynapses_byNumber(5,15,10,200,200,synBranches,synLoc)
//This is to specify how many synapses to insert in proximal, medial and distal dendrites.
//WTD2
// AddExcSynapses_byNumber(5,14,10,200,200,synBranches,synLoc)
//HETD1
// AddExcSynapses_byNumber(4,13,10,200,200,synBranches,synLoc)
//HETD2
// AddExcSynapses_byNumber(3,14,11,200,200,synBranches,synLoc)
// nSynapse = AddExcSynapses_byNumber(10,10,10,200,200,synBranches,synLoc)
nSynapse = nASyn + nBSyn + nCSyn
// endSyn = 200 + (nSynapse + 2)*200
endSyn = 30000
distance()
cnt = 0
tstop=endSyn
//WTD1_Nov9IR2a
synTweak(nSynapse,2.0053,1.5669,.000268,"fig11_PFCapic")
//This is to specify the rise/decay time constants and max AMPAR conductance.
connEsyndend.record(tvec,idvec)
sprint(synFilename,"test_raster.txt")
//The test_raster file contains all the time for EPSC and corresponding activated synapses.
synFout = new File()
synFout.wopen(synFilename)
for j=0, tvec.size-1 synFout.printf("%7.3f \t%d\n", tvec.x(j), idvec.x(j))
synFout.close()
//printf("\n\n**************************\n\n")
//printf("Output has been written to a file ending in .Ibin, a customized binary file format.\n")
//printf("These files can be read with MATLAB. See the .m files contained in this directory.\n")
//printf("MATLAB's Statistics Toolbox is required to analyze EPSC shapes.\n")
//printf("\tSample usage: read_EPSCsims_mdb('fig11_PFCapic',0.00054)\n")
//printf("\n\n**************************\n\n")