//genesis float dt = 20e-6 str disk = "results/" int amp = {1 / dt + 1} str a = (amp) int i, k, l str el, name float t /********************************************************************* ** Simple Granule cell model script (#1) ** Carl Piaf BBF 1994 *********************************************************************/ str filename = (disk) @ "G_soma_test20pA" /* always include these default definitions! */ include defaults str cellpath = "/Golgi" /* Golgi cell constants */ include Golg_const.g /* special scripts to create the prototypes */ include Golg_chan_tab.g include Golg_synchan.g include Golg_comp.g /* Set the clocks */ for (i = 0; i <= 7; i = i + 1) setclock {i} {dt} end setclock 8 4.0e-5 setclock 9 1 /* To ensure that all subsequent elements are made in the library */ ce /library /* These make the prototypes of channels and compartments that can be ** invoked in .p files */ make_Golgi_chans make_Golgi_syns make_Golgi_comps /* call Gran_InNa TABSAVE tabInNa37.data call Gran_KDr TABSAVE tabKDr37.data call Gran_KA TABSAVE tabKA37.data call Gran_CaHVA TABSAVE tabCaHVA37.data call Gran_H TABSAVE tabH37.data call Moczyd_KC TABSAVE tabKCa37.data */ //make_Vmgraph /* create the model and set up the run cell mode */ // read cell data from .p file echo STARTING READ CELL readcell Golg1M0.p {cellpath} create neutral /library/interneuron/soma/mf_presyn disable /library/interneuron/soma/mf_presyn setfield /library/interneuron/soma/mf_presyn z 0 // Comment out whichever one to switch it off //addmsg /library/interneuron/soma/mf_presyn /Golgi/soma/GABAA ACTIVATION z //addmsg /library/interneuron/soma/mf_presyn /Golgi/soma/mf_NMDA ACTIVATION z addmsg /library/interneuron/soma/mf_presyn /Golgi/soma/mf_AMPA ACTIVATION z //read_hines -vm -method 11 Gran1M0.p {cellpath} /* Create the output element */ create asc_file /output/plot_out //create disk_out /output/plot_out useclock /output/plot_out 8 enable /output enable /output/plot_out ce {cellpath} // setup the hines solver echo preparing hines solver... create hsolve solve // if this is set then reset will NOT change Vm in Hines ce solve setfield . path "../##[][TYPE=compartment]" comptmode 1 chanmode 4 call . SETUP setmethod 11 /* el = ({findsolvefield {cellpath}/solve soma/H Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#2 el = ({findsolvefield {cellpath}/solve soma/Ca_pool Ca}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#3 */ el = ({findsolvefield {cellpath}/solve soma Vm}) //#4 addmsg {cellpath}/solve /output/plot_out SAVE {el} /* el = ({findsolvefield {cellpath}/solve soma/Moczyd_KC Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#5 el = ({findsolvefield {cellpath}/solve soma/KDr Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#6 el = ({findsolvefield {cellpath}/solve soma/InNa Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#7 el = ({findsolvefield {cellpath}/solve soma/KA Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#8 el = ({findsolvefield {cellpath}/solve soma/CaHVA Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#9 el = ({findsolvefield {cellpath}/solve soma/Moczyd_KC Gk}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#5 el = ({findsolvefield {cellpath}/solve soma/CaHVA Gk}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#9 el = ({findsolvefield {cellpath}/solve soma/mf_AMPA Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#10 el = ({findsolvefield {cellpath}/solve soma/mf_AMPA Gk}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#10 el = ({findsolvefield {cellpath}/solve soma/mf_NMDA Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#11 el = ({findsolvefield {cellpath}/solve soma/Mg_BLOCK Ik}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#12 el = ({findsolvefield {cellpath}/solve soma/Mg_BLOCK Gk}) addmsg {cellpath}/solve /output/plot_out SAVE {el} //#13 */ setfield /output/plot_out filename {filename} initialize 1 leave_open 1 \ flush 1 echo Output to {filename} //check reset /* step 0.175 -time // Synaptic stimulation protocol setfield /library/interneuron/soma/mf_presyn z {amp} step 1 setfield /library/interneuron/soma/mf_presyn z 0 step 325e-3 -time */ // Current injection protocol step 0.5 -time call {cellpath}/solve HGET {cellpath}/soma setfield {cellpath}/soma inject 20e-12 call {cellpath}/solve HPUT {cellpath}/soma step 0.5 -time call {cellpath}/solve HGET {cellpath}/soma setfield {cellpath}/soma inject 0e-12 call {cellpath}/solve HPUT {cellpath}/soma step 0.5 -time /* call {cellpath}/solve HGET {cellpath}/soma setfield {cellpath}/soma inject -22.5e-12 call {cellpath}/solve HPUT {cellpath}/soma step 0.5 -time */