// genesis include kons_olm.g /*****This script creates a prototype for the oriens interenuron which projects ******to the LM moleculare region innervating pyramidal cells in the distal ******apical dendrites ******/ create neutral /prot_olm //***************************************************************************** //*****Somatic compartment create compartment /prot_olm/soma setfield /prot_olm/soma \ Cm {CM_OLM * SOMA_A_OLM} \ // F Ra {RA_OLM * SOMA_L_OLM /SOMA_XA_OLM}\ // ohm (felesleges) Em {EREST_ACT_OLM} \ // V Rm {RM_OLM/SOMA_A_OLM} \ // ohm inject 0.0 \ initVm -0.065 //***************************************************************************** //*****Active sodium channel a la Wang & Buzsaki '96 with fast m gate create vdep_channel /prot_olm/soma/Na_channel setfield /prot_olm/soma/Na_channel \ Ek 55e-3 \ // V gbar { 350 * {SOMA_A_OLM} } // S //*****the m gate of the sodium channel create table /prot_olm/soma/Na_channel/m_gate ce /prot_olm/soma/Na_channel/m_gate call . TABCREATE {VRES} {VMIN} {VMAX} int i float y float alpham float betam float x echo Tablazat feltoltese ... for (i = 0; i<= VRES; i = i + 1) x = (i * (VMAX - VMIN) / VRES) + VMIN alpham= -0.1e+6 * ( x + 0.035) / ({ exp {-0.1e+3 * ( x + 0.035 )} } - 1) betam= 4e+3 * { exp { -1 * ( x + 0.060 ) / 0.018 } } y = alpham / ( alpham + betam ) if (x == -0.035) y = 0.9970947 end setfield . table->table[{i}] {y} end setfield . table->calc_mode 0 ce / //*****the h gate of the sodium channel create tabgate /prot_olm/soma/Na_channel/h_gate /*****tobb dolog is megkavarja a nagysagrandeket es elojeleket: atteres mV->V; ******atteres ms->s; az exponencialis szamlaloban, vagy nevezoben van-e ******/ setupgate ^ alpha {Phi*70} 0 0 58e-3 20e-3 -size {VRES} -range {VMIN} {VMAX} setupgate ^ beta {Phi*1e+3} 0 1 28e-3 -10e-3 -size {VRES} -range {VMIN} {VMAX} //*****Connecting gates to the channel ce /prot_olm/soma/Na_channel addmsg m_gate . MULTGATE output 3 addmsg h_gate . MULTGATE m 1 ce / //***************************************************************************** //*****Active potassium channel (deleyed rectifier) create vdep_channel /prot_olm/soma/K_channel setfield ^ Ek -90e-3 gbar {90*{SOMA_A_OLM}} create tabgate /prot_olm/soma/K_channel/nv_gate setupgate ^ alpha {-0.01e+6*Phi*0.034} {-0.01e+6*Phi} -1 0.034 -10e-3 -size {VRES} -range {VMIN} {VMAX} setupgate ^ beta {0.125e+3*Phi} 0 0 0.044 0.080 -size {VRES} -range {VMIN} {VMAX} //*****Connecting gates to the channel ce /prot_olm/soma/K_channel addmsg nv_gate . MULTGATE m 4 ce / //***************************************************************************** //*****Active hyperpolarization-activated channel (for the I_h current) create vdep_channel /prot_olm/soma/h_channel setfield ^ Ek -40e-3 gbar {1.5*{SOMA_A_OLM}} //*****H gate of the channel create tabgate /prot_olm/soma/h_channel/H_gate //create table /prot_olm/soma/h_channel/H_gate ce /prot_olm/soma/h_channel/H_gate call . TABCREATE alpha {VRES} {VMIN} {VMAX} call . TABCREATE beta {VRES} {VMIN} {VMAX} float alpha, beta for (i = 0; i <= VRES; i = i + 1) x = (i * (VMAX - VMIN) / VRES) + VMIN alpha = 1 / (1 + { exp {(x + 80e-3) / 10e-3 }}) / (200e-3/({ exp {(x + 70e-3) /20e-3}} + { exp {-(x + 70e-3) / 20e-3}})+0.005) beta = (1 - 1 / (1 + { exp {(x + 80e-3) / 10e-3 }})) / (200e-3/({ exp {(x + 70e-3) /20e-3}} + { exp {-(x + 70e-3) / 20e-3}})+0.005) setfield . alpha->table[{i}] {alpha} setfield . beta->table[{i}] {beta} end ce /prot_olm/soma/h_channel addmsg H_gate . MULTGATE m 1 ce / //***************************************************************************** //*****Calcium channel create vdep_channel /prot_olm/soma/Ca_channel setfield ^ Ek 120e-3 gbar {10 * {SOMA_A_OLM}} //*****The m gate of the channel create table /prot_olm/soma/Ca_channel/m_gate ce /prot_olm/soma/Ca_channel/m_gate call . TABCREATE {VRES} {VMIN} {VMAX} for (i = 0; i<= VRES; i = i + 1) x = (i * (VMAX - VMIN) / VRES) + VMIN y = 1/(1 + { exp {-(x + 20e-3) / 9e-3}}) setfield . table->table[{i}] {y} end setfield . table->calc_mode 0 ce /prot_olm/soma/Ca_channel addmsg m_gate . MULTGATE output 2 ce / //***************************************************************************** //*****Hyperpolarization activated Calcium-dependent potassium current //*****Calcium concentration create Ca_concen /prot_olm/soma/Calcium_c setfield ^ tau 0.08 B 200000 Ca_base 0 create vdep_channel /prot_olm/soma/K_C_channel setfield ^ Ek -90e-3 gbar {100 * {SOMA_A_OLM}} create table /prot_olm/soma/K_C_channel/psg ce ^ call . TABCREATE 3200 0 32e-7 for (i = 0; i <= 3200; i = i + 1) x = (i * (32e-7 - 0) / 3200) + 0 y = x / (x + 30e-6) setfield . table->table[{i}] {y} end setfield . table->calc_mode 0 ce /prot_olm/soma/K_C_channel addmsg psg . MULTGATE output 1 ce / //***************************************************************************** //Copying constituents to a prototype cell ce /prot_olm/soma addmsg . K_channel/nv_gate VOLTAGE Vm addmsg K_channel . CHANNEL Gk Ek addmsg . K_channel VOLTAGE Vm addmsg . Na_channel/m_gate INPUT Vm addmsg . Na_channel/h_gate VOLTAGE Vm addmsg Na_channel . CHANNEL Gk Ek addmsg . Na_channel VOLTAGE Vm addmsg . h_channel/H_gate VOLTAGE Vm addmsg h_channel . CHANNEL Gk Ek addmsg . h_channel VOLTAGE Vm addmsg . Ca_channel/m_gate INPUT Vm addmsg Ca_channel . CHANNEL Gk Ek addmsg . Ca_channel VOLTAGE Vm addmsg Ca_channel Calcium_c I_Ca Ik addmsg Calcium_c K_C_channel/psg INPUT Ca addmsg K_C_channel . CHANNEL Gk Ek addmsg . K_C_channel VOLTAGE Vm ce /