// Author: Etay Hay, 2013
// Preserving axosomatic spiking features despite diverse dendritic morphology (Hay et al., 2013, J.Neurophysiology)
//
// Template for models of L5 Pyramidal Cell
begintemplate L5PCtemplate
public init
public locateSites, getLongestBranch, delete_axon2
public soma, dend, apic, axon, getAbsSecIndex, myelin
public all, somatic, apical, axonal, basal, nSecSoma, nSecApical, nSecBasal, nSecAxonal, nSecAll, nSecAxonalOrig, SecSyn, distribute_channels
objref SecSyn, this
objref all, somatic, apical, axonal, basal
strdef tstr
//$s1 - morphology file name
proc init() {localobj nl,import
all = new SectionList()
somatic = new SectionList()
basal = new SectionList()
apical = new SectionList()
axonal = new SectionList()
forall delete_section()
nl = new Import3d_Neurolucida3()
nl.quiet = 1
nl.input($s1)
import = new Import3d_GUI(nl, 0)
import.instantiate(this)
geom_nseg()
biophys()
forsec this.all {
if(diam == 0){
diam = 1
printf("Error : Morphology problem with section [%s] 0 diam \n", secname())
}
}
}
create soma[1], dend[1], apic[1], axon[1], myelin[1]
proc geom() {
}
proc geom_nseg() {local nSec, L1, L2, D1, D2, nSeg1, nSeg2
soma area(.5) // make sure diam reflects 3d points
nSec = 0
forsec all {
nseg = 1 + 2*int(L/40)
nSec = nSec + 1
}
nSecAll = nSec
nSec = 0
forsec somatic { nSec = nSec + 1}
nSecSoma = nSec
nSec = 0
forsec apical { nSec = nSec + 1}
nSecApical= nSec
nSec = 0
forsec basal { nSec = nSec + 1}
nSecBasal = nSec
nSec = 0
forsec axonal { nSec = nSec + 1}
nSecAxonalOrig = nSecAxonal = nSec
}
proc biophys() {localobj bp
delete_axon()
area(0.5)
distance()
access soma
bp = new L5PCbiophys()
bp.biophys(this)
}
// deleting axon, keeping only first 60 micrometers
proc delete_axon(){
forsec axonal{delete_section()}
create axon[2]
access axon[0]{
L= 30
diam = 1
nseg = 1+2*int(L/40)
all.append()
axonal.append()
}
access axon[1]{
L= 30
diam = 1
nseg = 1+2*int(L/40)
all.append()
axonal.append()
}
nSecAxonal = 2
connect axon(0), soma(0.5)
connect axon[1](0), axon[0](1)
access soma
}
//deleting axon, appending instead a standard axon
//$1-4: parameters of axonal tapering
//$1 d0, diameter at axon base
//$2 d1, diameter at axon 20 micrometers from soma
//$3 d2, diameter at axon 50 micrometers from soma
//$4 d3, diameter of myelin section
proc delete_axon2(){
forsec axonal{delete_section()}
create axon[2]
access axon[0]{
L= 20
nseg = 1+2*int(L/10)
diam(0:1) = $1:$2
all.append()
axonal.append()
}
access axon[1]{
L= 30
nseg = 1+2*int(L/10)
diam(0:1) = $2:$3
all.append()
axonal.append()
}
if($4){
create myelin[1]
access myelin{
L = 1000
diam = $4
nseg = 1+2*int(L/100)
}
connect myelin(0), axon[1](1)
}
nSecAxonal = 2
connect axon(0), soma(0.5)
connect axon[1](0), axon[0](1)
access soma
}
proc distribute_channels() {local dist,val,base,maxLength
base = $8
soma distance()
maxLength = getLongestBranch($s1)
forsec $s1 {
if(0==strcmp($s2,"Ra")){
Ra = $8
} else {
for(x) {
if ($3==3) {
dist = distance(x)
} else {
dist = distance(x)/maxLength
}
val = calculate_distribution($3,dist,$4,$5,$6,$7,$8)
sprint(tstr,"%s(%-5.10f) = %-5.10f",$s2,x,val)
execute(tstr)
}
}
}
}
// $1 is the distribution type:
// 0 linear, 1 sigmoid, 2 exponential
// 3 step for absolute distance (in microns)
func calculate_distribution() {local value
if ($1==0) {value = $3 + $2*$4}
if ($1==1) {value = $3 + ($4/(1+exp(($2-$5)/$6)))}
if ($1==2) {value = $3 + $6*exp($4*($2-$5))}
if ($1==3) {
if (($2 > $5) && ($2 < $6)) {
value = $3
} else {
value = $4
}
}
value = value*$7
return value
}
// $s1 section
func getLongestBranch(){local maxL,d localobj distallist,sref
sprint(tstr,"%s distance()",$s1)
execute(tstr,this)
if(0==strcmp($s1,"axon")){
sprint(tstr,"%s[0] distance(1)",$s1)
execute(tstr,this)
}
maxL = 0
d = 0
distallist = new SectionList()
forsec $s1 {
sref = new SectionRef()
if (sref.nchild==0) distallist.append()
}
forsec distallist{
d = distance(1)
if(maxL<d) maxL = d
}
// for the soma case
if (maxL == 0) {
$s1 {
maxL = L
}
}
return maxL
}
// $s1 section
// $2 distance x in micrometers
// return list of [1,2] vectors - of the appropriate section and the location in each vector
obfunc locateSites() {local maxL,site,d0,d1,siteX,i localobj vv,ll
ll = new List()
sprint(tstr,"%s distance()",$s1)
execute(tstr,this)
if(0==strcmp($s1,"axon")){
sprint(tstr,"%s[0] distance(1)",$s1)
execute(tstr,this)
}
maxL = getLongestBranch($s1)
site = $2
i = 0
forsec $s1 {
if (distance(0) < distance(1)) {
d0 = distance(0)
d1 = distance(1)
} else {
d1 = distance(0)
d0 = distance(1)
}
if (site <= d1 && site >= d0) {
siteX = (site-d0)/(d1-d0)
secNum = i
vv = new Vector()
ll.append(vv.append(secNum,siteX))
}
i = i+1
}
return ll
}
func getAbsSecIndex(){ local nAbsInd, index localobj str,strObj
strObj = new StringFunctions()
str = new String()
nAbsInd = 0
index = 0
if(strObj.substr($s1, "soma") > 0) {
strObj.tail($s1, "soma", str.s)
if(sscanf(str.s, "%*c%d", &index) < 0) {
index = 0
}
nAbsInd = index
}else if (strObj.substr($s1, "axon") >0) {
strObj.tail($s1, "axon", str.s)
if(sscanf(str.s, "%*c%d", &index) < 0) {
index = 0
}
nAbsInd = nSecSoma + index
}else if (strObj.substr($s1, "dend") >0) {
strObj.tail($s1, "dend", str.s)
if(sscanf(str.s, "%*c%d", &index) < 0) {
index = 0
}
nAbsInd = nSecSoma + nSecAxonalOrig + index
}else if (strObj.substr($s1, "apic") > 0) {
strObj.tail($s1, "apic", str.s)
if(sscanf(str.s, "%*c%d", &index) < 0) {
index = 0
}
nAbsInd = nSecSoma + nSecAxonalOrig + nSecBasal + index
}
return nAbsInd
}
endtemplate L5PCtemplate