//---------------------------------------------------------------------------------------------------------------------------------//
// Localization of intracellular stimulation at the soma and Ca-PIC channels over dendrites at the similar distance from the soma //
//---------------------------------------------------------------------------------------------------------------------------------//
// global constants
v_init=-70
num_row=10000
// global variables
objref iCaL[num_row]
objref istim
// localization of Ca-PIC channels at the similar Dpath
proc loc_dend(){local max_dist, err_min, dpath, init, end, err_dist, x_min, j
max_dist=0
err_min=0
init=0
end=0
err_dist=0
x_min=0
j=0
// calculate the longest Dpath from the soma
forall {
soma distance(0, 0)
for(x){
if (max_dist < distance(x)) {
max_dist = distance(x)
}
}
err_min=max_dist
}
// set the specific Dpath
dpath = 800
forall {
init = distance(0)
end = distance(1)
if (init<=dpath && dpath<end) {
for(x) {
err_dist=abs(dpath-distance(x))
if (err_min > err_dist) {
err_min = err_dist
x_min=x
}
}
if(x_min==0){x_min=0.0001}
if(x_min==1){x_min=0.9999}
iCaL[j] = new CaL(x_min)
//iCaL[j].gcalbar=1.57 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 100um
//iCaL[j].gcalbar=1.14 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 200um
//iCaL[j].gcalbar=1.21 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 300um
//iCaL[j].gcalbar=1.25 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 400um
//iCaL[j].gcalbar=1.28 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 500um
//iCaL[j].gcalbar=1.37 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 600um
//iCaL[j].gcalbar=1.39 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 700um
iCaL[j].gcalbar=1.95 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 800um
//iCaL[j].gcalbar=2.8 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 900um
//iCaL[j].gcalbar=4.1 * area(x_min) * (10^-4)^2 * 10^3 //[mS/cm2] for dpath = 1000um
j=j+1
err_min=max_dist
}
}
}
loc_dend()
// Intracellular current injection at the soma
access soma
istim = new SawtoothIClamp(0.5)
istim.tp = 2000
istim.pkamp = 15
istim.bias = -5