TITLE Low threshold calcium current
:
: Ca++ current responsible for low threshold spikes (LTS)
: RETICULAR THALAMUS - custom channel
: Differential equations
:
: Adapted from Model of Huguenard & McCormick, J Neurophysiol 68: 1373-1383, 1992.
: The kinetics is described by standard equations (NOT GHK)
: using a m2h format, according to the voltage-clamp data
: (whole cell patch clamp) of Huguenard & Prince, J Neurosci.
: 12: 3804-3817, 1992.
:
: - Kinetics adapted to fit the T-channel of reticular neuron
: - Time constant tau_h refitted from experimental data
: - shift parameter for screening charge
:
: Model described in detail in:
: Destexhe, A., Contreras, D., Steriade, M., Sejnowski, T.J. and
: Huguenard, J.R. In vivo, in vitro and computational analysis of
: dendritic calcium currents in thalamic reticular neurons.
: Journal of Neuroscience 16: 169-185, 1996.
: See also:
: http://www.cnl.salk.edu/~alain
: http://cns.fmed.ulaval.ca
:
: Written by Alain Destexhe, Salk Institute, Sept 18, 1992
:
: Trivial modification by Andrew Knox to include taubase variable
INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)}
NEURON {
SUFFIX itrecustom
USEION ca READ cai, cao WRITE ica
RANGE gcabar, m_inf, tau_m, h_inf, tau_h, shift, qm, qh, taubase
}
UNITS {
(molar) = (1/liter)
(mV) = (millivolt)
(mA) = (milliamp)
(mM) = (millimolar)
FARADAY = (faraday) (coulomb)
R = (k-mole) (joule/degC)
}
PARAMETER {
v (mV)
celsius = 36 (degC)
: eca = 120 (mV)
gcabar = .003 (mho/cm2)
shift = 0 (mV)
taubase (mV)
cai = 2.4e-4 (mM) : adjusted for eca=120 mV
cao = 2 (mM)
qm = 2.5
qh = 2.5
}
STATE {
m h
}
ASSIGNED {
ica (mA/cm2)
carev (mV)
m_inf
tau_m (ms)
h_inf
tau_h (ms)
phi_m
phi_h
}
BREAKPOINT {
SOLVE castate METHOD cnexp
carev = (1e3) * (R*(celsius+273.15))/(2*FARADAY) * log (cao/cai)
ica = gcabar * m*m*h * (v-carev) : original function
: ica = gcabar * m_inf * m_inf * h * (v-carev)
}
DERIVATIVE castate {
evaluate_fct(v)
m' = (m_inf - m) / tau_m
h' = (h_inf - h) / tau_h
}
UNITSOFF
INITIAL {
:
: Activation functions and kinetics were obtained from
: Huguenard & Prince, and were at 23-25 deg.
: Transformation to 36 deg using Q10
:
phi_m = qm ^ ((celsius-24)/10)
phi_h = qh ^ ((celsius-24)/10)
evaluate_fct(v)
m = m_inf
h = h_inf
}
PROCEDURE evaluate_fct(v(mV)) {
:
: Time constants were obtained from J. Huguenard
:
: original steady state activation/inactivation
m_inf = 1.0 / ( 1 + exp(-(v+shift+50)/7.4) )
h_inf = 1.0 / ( 1 + exp((v+shift+78)/5.0) )
tau_m = ( 3 + 1.0 / ( exp((v+shift+25)/10) + exp(-(v+shift+100)/15) ) )
tau_h = ( taubase + 1.0 / ( exp((v+shift+46)/4) + exp(-(v+shift+405)/50) ))
tau_h = tau_h / phi_h
tau_m = tau_m / phi_m
}
UNITSON