/* 4-compartment model of a deep layer PFC pyramidal cell */
/* based on Durstewitz et al. (2000), J.Neurophysiol. 83: 1733-50 updated by K. Sidiropoulou, 05/2014*/
begintemplate Pcell
public soma, dend, axon
create soma,dend[3], axon //dend[0]=basal, dend[1]=proximal, dend[2]=distal
proc init() {
ndend = 3
create soma, dend[ndend], axon
soma{
nseg=3
L=86.3
diam=10.14
insert pas
cm = 1.2 /* microF/cm2 */
g_pas = 9e-5 /* mho/cm2 */
e_pas = -65
Ra = 150 /* ohm-cm */
//conductances
soma_kdr = 0.018*0.3
soma_naf = 0.018
fast_kca = 0.0022
soma_nap = 0.000018
soma_Kslow = 0.0012
soma_kad = 0.0007
soma_calc = 0.0001*0.1
soma_cal = 0.0001*0.3
soma_can = 0.0002
soma_cat = 0.0002*0.3
soma_car = 0.000001*0.3
soma_h = 1.8e-5 *0.5
soma_kca = 0.005*5
ican_soma=0.001*0.07*0
ican_dend=ican_soma*0.1*0
insert Naf
gnafbar_Naf = soma_naf*6
insert Nap
gnapbar_Nap = soma_nap*0.1
insert calc
gcabar_calc = soma_calc
insert cal
gcalbar_cal = soma_cal
insert can
gcabar_can = soma_can*0.1
insert car
gcabar_car = soma_car*0.1
insert cat
gcatbar_cat = soma_cat*0.1
insert kdr
gkdrbar_kdr = soma_kdr
insert IKs
gKsbar_IKs = soma_Kslow*0.5
insert kad
gkabar_kad = soma_kad
insert iC
gkcbar_iC = fast_kca
insert h
gbar_h = soma_h
insert kca
gbar_kca = soma_kca
insert ican
gbar_ican=ican_soma
insert cadyn
}
//Dendritic_factors
dend[0]{
//basal dendrites
nseg=9
L=150.0
diam=1
insert pas
cm = 1.2*2 /* microF/cm2 */
g_pas = 8.5e-5*2 /* mho/cm2 */
e_pas = -65
Ra = 150 /* ohm-cm */
insert Naf
gnafbar_Naf = soma_naf*0.1
insert Nap
gnapbar_Nap = soma_nap
insert calc
gcabar_calc = soma_calc
insert cal
gcalbar_cal = soma_cal
insert can
gcabar_can = soma_can*0.3
insert car
gcabar_car = soma_car*0.3
insert cat
gcatbar_cat = soma_cat
insert kdr
gkdrbara_kdr = soma_kdr*0.09
insert IKs
gKsbar_IKs = soma_Kslow
insert kad
gkabar_kad = soma_kad
insert iC
gkcbar_iC = fast_kca*0.001
insert kca
gbar_kca = soma_kca*0.001
insert h
gbar_h =soma_h
insert ican
gbar_ican=ican_dend
insert cadyn
}
dend[1]{
//proximal dendrites
nseg=17
L=400.0
diam=2.6
insert pas
cm = 1.2*2 /* microF/cm2 */
g_pas = 8.5e-5*2 /* mho/cm2 */
e_pas = -65
Ra = 150 /* ohm-cm */
insert Naf
gnafbar_Naf = soma_naf*0.4
insert Nap
gnapbar_Nap = soma_nap*3
insert calc
gcabar_calc = soma_calc
insert can
gcabar_can = soma_can*0.3
insert car
gcabar_car = soma_car*0.3
insert cat
gcatbar_cat = soma_cat
insert kdr
gkdrbar_kdr = soma_kdr*0.004
insert IKs
gKsbar_IKs = soma_Kslow
insert kad
gkabar_kad = soma_kad
insert iC
gkcbar_iC = fast_kca*0.01
insert kca
gbar_kca = soma_kca*0.1
insert h
gbar_h =soma_h
insert ican
gbar_ican=ican_dend
insert cadyn
}
dend[2]{
nseg=5
L=400
diam=2.6
insert pas
cm = 1.2*2 /* microF/cm2 */
g_pas = 8.5e-5*2 /* mho/cm2 */
e_pas = -65
Ra = 150 /* ohm-cm */
insert Naf
gnafbar_Naf = soma_naf*0.2
insert Nap
gnapbar_Nap = soma_nap*10
insert calc
gcabar_calc = soma_calc*0.3
insert can
gcabar_can = soma_can*5
insert car
gcabar_car = soma_car*5
insert cat
gcatbar_cat = soma_cat*0.1
insert kdr
gkdrbar_kdr = soma_kdr*0.001
insert IKs
gKsbar_IKs = soma_Kslow
insert kad
gkabar_kad = soma_kad*0.1
insert iC
gkcbar_iC = fast_kca*0.001
insert h
gbar_h =soma_h*10
insert ican
gbar_ican=ican_dend
insert cadyn
}
axon {
nseg=1
L=113.22
diam=1.1
//insert kdyn
insert pas
cm = 1.2 /* microF/cm2 */
g_pas = 8.5e-5 /* mho/cm2 */
e_pas = -65
Ra = 150 /* ohm-cm */
insert Naf
gnafbar_Naf=soma_naf*10
insert kdr
gkdrbar_kdr=soma_kdr
}
connect dend[0](0), soma(0)
connect dend[1](0), soma(1)
connect dend[2](0), dend[1](1)
connect axon(0), soma(0.5)
cao0_ca_ion = 2 /* mM */
cai0_ca_ion = 50e-6 /* mM */
ko0_k_ion = 3.82 /* mM */
ki0_k_ion = 140 /* mM */
celsius = 23
}
init()
endtemplate Pcell
//Creating new neurons
nPcells = 16//9
objref Pcells[nPcells]
for i = 0, (nPcells-1) {
Pcells[i] = new Pcell()
}
//Create a list with segments
objref soma_list, dend_list, pcell_list, cell0_list
soma_list = new SectionList()
for i=0, (nPcells-1) {
Pcells[i].soma soma_list.append()
}
dend_list = new SectionList()
for i=0, (nPcells-1) {Pcells[i].dend[0] dend_list.append()}
for i=0, (nPcells-1) {Pcells[i].dend[1] dend_list.append()}
for i=0, (nPcells-1) {Pcells[i].dend[2] dend_list.append()}
pcell_list = new SectionList()
for i=0, (nPcells-1) {Pcells[i].soma pcell_list.append()}
for i=0, (nPcells-1) {Pcells[i].dend[0] pcell_list.append()}
for i=0, (nPcells-1) {Pcells[i].dend[1] pcell_list.append()}
for i=0, (nPcells-1) {Pcells[i].dend[2] pcell_list.append()}
cell0_list=new SectionList()
Pcells[0].soma cell0_list.append()
Pcells[0].dend[0] cell0_list.append()
Pcells[0].dend[1] cell0_list.append()
Pcells[0].dend[2] cell0_list.append()
xopen("../bash_templates/current-balance.hoc")
current_balance(-65)