/*
* Procedures for launching desired cell model, scaling diameters/lengths, and adding myelinated axon
* AUTHOR: Aman Aberra, Duke University
* CONTACT: aman.aberra@duke.edu
*/
cell_id = 0 // leave unset until user selects cell
objref cell_names, cell, nil
objref main_ax_list, strobj
objref secnames[numSect]
min_sec_ind = 0
cell_names = new List()
strdef tstr1, tstr2, cell_dir, current_dir,createsim_file // read Traub template names into cell_names List
current_dir = getcwd()
for i=1,25 {getstr(tstr1,1) cell_names.append(new String(tstr1))}
L1_NGC-DA_bNAC219_1
L1_NGC-DA_bNAC219_2
L1_NGC-DA_bNAC219_3
L1_NGC-DA_bNAC219_4
L1_NGC-DA_bNAC219_5
L23_PC_cADpyr229_1
L23_PC_cADpyr229_2
L23_PC_cADpyr229_3
L23_PC_cADpyr229_4
L23_PC_cADpyr229_5
L4_LBC_cACint209_1
L4_LBC_cACint209_2
L4_LBC_cACint209_3
L4_LBC_cACint209_4
L4_LBC_cACint209_5
L5_TTPC2_cADpyr232_1
L5_TTPC2_cADpyr232_2
L5_TTPC2_cADpyr232_3
L5_TTPC2_cADpyr232_4
L5_TTPC2_cADpyr232_5
L6_TPC_L4_cADpyr231_1
L6_TPC_L4_cADpyr231_2
L6_TPC_L4_cADpyr231_3
L6_TPC_L4_cADpyr231_4
L6_TPC_L4_cADpyr231_5
// cell_chooser(cell_id)
proc cell_chooser() { local start_ind, synapses_enabled localobj terminal_sec_str, meth1cells
cell_id = $1 // reset cell_id to input argument
if (cell_id > 0){
forall delete_section()
//cell = nil
// Launch cell using create_cell within Blue Brain code
synapses_enabled = 0 // turn off synapses
sprint(cell_dir,"cells/%s",cell_names.o(cell_id-1).s)
chdir(cell_dir)
{load_file("createsimulation.hoc")} // load cell-specific createsimulation.hoc file
create_cell(synapses_enabled)
printf("*---------*\nLoaded Blue Brain cell: %s\n",cell_names.o(cell_id-1).s)
// insert xtra/extracellular and run setpointers/get secrefs()
forall { // call setpointers() on original cell to get type_xtra of each original axon section
insert xtra
insert extracellular
}
// Scale diameters and myelinate axon before setting pointers
// Scale compartment diameters
if (scale_soma_area != 1) diam(0.5) = scale_soma_area*area(0.5)/(PI*L)
if (scale_apic_diam != 1) scale_diam2(scale_apic_diam,cell.apical)
if (scale_basal_diam != 1) scale_diam2(scale_basal_diam,cell.basal)
if (scale_basal_L != 1) forsec cell.basal L=L*scale_basal_L
if (scale_axon_diam != 1) scale_diam2(scale_axon_diam,cell.axonal)
// get coordinates and secrefs list
getSecRefs()
// Get main axon of original axon
// Prune axon if prune_ax >=1 for BLUE-BRAIN cells
meth1cells = new Vector() // identify which main axon identification algorithm to use
meth1cells.append(6,8,20,21,22,25) // method 1 cell ids
// get main axon (most relevant for PCs) and prune sections (non-main axon axonal sections)
objref main_ax_list
// defines main_ax_list and min_sec_ind (public variable)
if (!meth1cells.contains(cell_id)) { // use descending approach for most axons
main_ax_list = get_main_ax2()
print "Got main axon (method 2)"
} else { // for cell meth1cells use ascending approach
main_ax_list = get_main_ax()
print "Got main axon (method 1)"
}
// Myelinate axon after getting main axon, pruning, and scaling diameter
if (myelinate_ax) {
myelinate_axon(cell.axonal)
numComp = 0
forall {
if (myelinate_ax){ // re-insert for myelin
insert xtra
insert extracellular
}
numComp+= nseg // save number of compartments
}
setpointers()
printf("Inserted xtra and extracellular in all %g compartments\n",numComp)
//setpointers()
// get new main axon
if (!meth1cells.contains(cell_id)) { // use descending approach for most axons
main_ax_list = get_main_ax2()
print "Got main axon (method 2)"
} else { // for meth1cells use ascending approach
setpointers()
main_ax_list = get_main_ax()
print "Got main axon (method 1)"
}
} else {
// get numComp
numComp = 0
forall {
insert xtra
insert extracellular
numComp+= nseg // save number of compartments
}
setpointers()
}
chdir(current_dir) // switch back to parallel_plate
print "Cell Loaded"
// Initilize potentials and waveform
// getes() // set Ve(x,y,z)
// setstim(DEL,DUR,AMP) // set waveform (current of E-field)
color_plotmax()
} else {
print "Cell model not selected"
}
}
// Get main axon using ascending approach - starting from lowest z point and going to AIS
// objref main_ax
// main_ax = get_main_ax()
// also outputs min_sec_ind, access using secrefs.o(min_sec_ind)
main_ax_rad = 500 // um main descending axon should be within this radius
obfunc get_main_ax() { local i, miny, min_ind, xi, yi, zi, at_root localobj main_ax, current_secref
main_ax = new SectionList() // main axon sections
// Find lowest point of axon (on z axis)
miny = 100 // initialize
for i = 0,numSect-1 {
if (yList.o(i).min < miny){
min_ind = yList.o(i).min_ind
xi = xList.o(i).x[min_ind]
yi = yList.o(i).x[min_ind]
rdist = sqrt((xi-secrefs.o(0).sec.x_xtra(0.5))^2+ (zi-secrefs.o(0).sec.y_xtra(0.5))^2 )
if (rdist < main_ax_rad){ // make sure compartment is not an oblique, descending collateral by ensuring it's within a horizontal radius
miny = yList.o(i).min
min_sec_ind = i // get index of section with lowest z-value, corresponds to terminal compartment
}
}
}
// ascend axon
at_root = 0
current_secref = secrefs.o(min_sec_ind)
while (!at_root) { // loop until reach root
if (current_secref.has_parent){
current_secref.sec main_ax.append() // add to SectionList
current_secref.parent {
current_secref = new SectionRef() // make parent current secref
}
} else {
at_root = 1
}
}
return main_ax
}
// Get main axon using descending approach - choose biggest of branches
obfunc get_main_ax2() { local i, biggest_branch_diam, biggest_branch_ind, at_terminal localobj main_ax, current_secref, terminal_sec_str, current_sec_str
main_ax = new SectionList() // main axon sections
// descend axon
at_terminal = 0
cell.axon[0] current_secref = new SectionRef() // start at cell.axon[0] (initial segment/axon hillock)
while (!at_terminal) { // loop until reach root
current_secref.sec main_ax.append() // add to SectionList
if (current_secref.nchild > 0){
biggest_branch_diam = 0 // diameter of biggest child branch
biggest_branch_ind = 0 // index of biggest child branch among current_secref's children
for i = 0,current_secref.nchild - 1 {
if(current_secref.child[i].diam(0) > biggest_branch_diam) {
biggest_branch_diam = current_secref.child[i].diam(0)
biggest_branch_ind = i
}
}
current_secref.child[biggest_branch_ind] { // make biggest child branch new current_sec, append to main-ax
current_secref = new SectionRef() // make child current secref
}
} else {
at_terminal = 1
terminal_sec_str = new String()
current_secref.sec terminal_sec_str.s = secname()
current_sec_str = new String()
for i = 0, numSect - 1 {
secrefs.o(i).sec current_sec_str.s = secname()
if (strcmp(current_sec_str.s,terminal_sec_str.s) == 0){ // compare each secname to terminal secname to get index - 0 means identical
min_sec_ind = i
//printf("Terminal section: %s, index: %g\n",terminal_sec_str.s,min_sec_ind)
}
}
}
}
return main_ax
}