TITLE BK Ca 2+ -activated K + channel
: Calcium activated K channel.
COMMENT
Starting from the formulation in De Schutter and Bower, 1994, we
reduced the Ca 2+ dependent activation time to half to account for the larger slow repolarisation at
depolarised states.
Current Model Reference: Karima Ait Ouares , Luiza Filipis , Alexandra Tzilivaki , Panayiota Poirazi , Marco Canepari (2018) Two distinct sets of Ca 2+ and K + channels
are activated at different membrane potential by the climbing fibre synaptic potential in Purkinje neuron dendrites.
Kinetics were fit to data from Filipis et al. 2022
PubMed link:
Contact: Filipis Luiza (luiza.filipis@univ-grenoble-alpes.fr)
ENDCOMMENT
UNITS {
(molar) = (1/liter)
}
UNITS {
(mV) = (millivolt)
(mA) = (milliamp)
(mM) = (millimolar)
}
INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)}
NEURON {
SUFFIX bks
USEION lca READ lcai
USEION nca READ ncai
USEION tca READ tcai
USEION k READ ek WRITE ik
RANGE gkbar,gk,zinf,ik, minf
GLOBAL bkcoef,bkexp,cahco, carco,tin, tinsh, calco
}
PARAMETER {
celsius=37 (degC)
v (mV)
gkbar=.08 (mho/cm2) : Maximum Permeability
ncai (mM)
lcai (mM)
tcai (mM)
ek (mV)
dt (ms)
tin=0.02
bkcoef=0.023
bkexp=7.5
cahco=1
carco=0
calco=1
tinsh=400
}
ASSIGNED {
ik (mA/cm2)
minf
mexp
zinf
zexp
gk
}
STATE { m z } : fraction of open channels
BREAKPOINT {
SOLVE state
: gk = gkbar*1000*m*z*z
ik = gkbar*1000*m*z*z*(v - ek)
}
:UNITSOFF
:LOCAL fac
:if state_cagk is called from hoc, garbage or segmentation violation will
:result because range variables won't have correct pointer. This is because
: only BREAKPOINT sets up the correct pointers to range variables.
PROCEDURE state() { : exact when v held constant; integrates over dt step
rate(v, lcai,ncai,tcai)
m = m + mexp*(minf - m)
z = z + zexp*(zinf - z)
VERBATIM
return 0;
ENDVERBATIM
}
INITIAL {
rate(v, lcai,ncai, tcai)
m = minf
z = zinf
}
FUNCTION alp(v (mV), lcai (mM), ncai (mM), tcai(mM)) (1/ms) { :callable from hoc
alp = 0.4/((lcai*calco+ncai*cahco+tcai*carco))
}
FUNCTION bet(v (mV)) (1/ms) { :callable from hoc
bet = 0.11/exp((v-55)/14.9)
}
PROCEDURE rate(v (mV), lcai (mM),ncai (mM), tcai (mM)) { :callable from hoc
LOCAL a,b,tinca
a = alp(v,lcai,ncai, tcai)
zinf = 1/(1+a)
:printf("zinf=%15.10g a=%15.10g\n",zinf,a)
zexp = (1 - exp(-dt/4))
b = bet(v)
:minf = 8.5/(7.5+b)
minf = 1/(1+b)
mexp = (1 - exp(-dt*(bkexp+b)))
}
:UNITSON