\section{Distributed and point input to a segment} In general, segments receive distributed and point sources of input each of which require a different mathematical treatment. The current supplied by distributed input such as intrinsic voltage-dependent current or capacitative current is proportional to the surface area of the segment on which it acts, whereas the current supplied to a segment at a synapse or by an exogenous point input is independent of the size of the segment. An implicit assumption of a compartmental model is that distributed current input to a segment is small by comparison with axial current flowing along the segment. To appreciate why this assumption is reasonable, consider a cylindrical dendritic segment of radius $r$ (cm), length $h$ and with membrane of constant conductance $g_\mathrm{M}$ (mS/cm$^2$). Suppose that axoplasm has constant conductance $g_\mathrm{A}$ (mS/cm) and that a potential difference $V$ (mV) exists between the segment boundaries, then the axial current along the segment is $I_\mathrm{A}=\pi r^2 g_\mathrm{A} V/h$ ($\mu$A) and the total distributed current crossing the membrane of the segment is $I_\mathrm{M}=2\pi r h g_\mathrm{M}\,(V/2)$. The ratio of the distributed current to the axial current is therefore \begin{equation}\label{pc1} \frac{\mbox{Distributed current}}{\mbox{Axial current}} =\frac{I_\mathrm{M}}{I_\mathrm{A}}=\frac{\pi r h g_\mathrm{M}\,V} {\pi r^2 g_\mathrm{A}\,(V/h)}=\frac{h^2 g_\mathrm{M}} {r g_\mathrm{A}}=\Big(\frac{h}{r}\Big)^2\, \frac{r g_\mathrm{M}}{g_\mathrm{A}}\,. \end{equation} For a typical dendritic segment $r g_\mathrm{M}/g_\mathrm{A}$ is small (say $ \approx 10^{-5}$), and therefore distributed current acting on a segment is small by comparison with axial current for ``short'' segments. On the other hand, segments several orders of magnitude longer than their radius can be expected to have distributed and axial currents of similar magnitude. An important property of a compartmental model is that segments are not excessively long by comparison with their radius. In the treatment of distributed current, the development of the new compartmental model makes explicit use of the assumption that distributed current is much smaller than axial current. Since this assumption may not be valid for point sources of current, it will not be made for the treatment of these current in the new compartmental model. \subsection{Axial current in the absence of distributed and point current input} Figure \ref{model} illustrates a dendritic segment of length $h$ (cm) where $\lambda\in[0,1]$ is the fractional distance of a point of the segment from its proximal end ($\lambda=0$). Let $r_\mathrm{P}$ and $r_\mathrm{D}$ be the radii of the segment at its proximal and distal boundaries respectively, let $V_\mathrm{P}(t)$ and $V_\mathrm{D}(t)$ be the membrane potentials at these boundaries and let $I_\mathrm{PD}$ be the axial current in the segment in the absence of transmembrane current. \begin{figure}[!h] \centering \begin{tabular}{c} \includegraphics[ ]{NewCompFig2.eps} \end{tabular}\qquad \begin{tabular}{p{2.55in}} \caption{\label{model} A segment of length $h$ (cm) is illustrated. In the absence of transmembrane current, membrane potentials $V_\mathrm{P}$ and $V_\mathrm{D}$ at the proximal and distal boundaries of the segment generate axial current $I_\mathrm{PD}$.} \end{tabular} \end{figure} %\begin{figure}[!h] %\centering %\begin{tabular}{c} %\begin{mfpic}[1][1]{-40}{140}{180}{300} %\headlen7pt %\pen{0.5pt} %\dotspace=4pt %\dotsize=1pt %\pen{1pt} %\dotspace=4pt %\dotsize=1.5pt %% %% LH cylinder %\parafcn[s]{-180,180,5}{(100-21*sind(t),240+28*cosd(t))} %\lines{(0,288),(100,268)} %\lines{(0,192),(100,212)} %% %% Partial cylinder on left %\dotted\parafcn[s]{0,180,5}{(36*sind(t),240+48*cosd(t))} %\parafcn[s]{0,180,5}{(-36*sind(t),240+48*cosd(t))} %% %% Annotation of LH cylinder %\dashed\arrow\lines{(0,240),(100,240)} %\tlabel[bl](50,250){\large $I_\mathrm{PD}$} %\tlabel[bc](0,250){$V_\mathrm{P}$} %\tlabel[cc](0,180){\large $\lambda=0$} %\tlabel[bc](0,295){\textsf{P}} %\arrow\lines{(0,235),(0,200)} %\tlabel[cr](-5,220){\textsf{$r_\mathrm{P}$}} %% %% Annotation of RH cylinder %\tlabel[bc](100,250){$V_\mathrm{D}$} %\tlabel[cc](100,180){\large $\lambda=1$} %\tlabel[cc](100,280){\textsf{D}} %\arrow\lines{(100,235),(100,216)} %\tlabel[cl](105,228){\textsf{$r_\mathrm{D}$}} %\arrow\lines{(60,228),(95,228)} %\arrow\lines{(40,228),(5,228)} %\tlabel[cc](50,228){$h$} %\end{mfpic} %\end{tabular}\qquad %\begin{tabular}{p{2.55in}} %\caption{\label{model} A segment of length $h$ (cm) is illustrated. In %the absence of transmembrane current, membrane potentials $V_\mathrm{P}$ %and $V_\mathrm{D}$ at the proximal and distal boundaries of the %segment generate axial current $I_\mathrm{PD}$.} %\end{tabular} %\end{figure} The membrane of the segment in Figure \ref{model} is formed by rotating the straight line PD about the axis of the dendrite to form the frustum of a cone of radius \begin{equation}\label{mp1} r(\lambda)=(1-\lambda)r_\mathrm{P}+\lambda r_\mathrm{D} \,,\qquad \lambda\in[0,1]\,. \end{equation} Assuming that the segment is filled with axoplasm of constant conductance $g_\mathrm{A}$ and that no current crosses its membrane, then the relationship between $V_\mathrm{P}$, $V_\mathrm{D}$ and $I_\mathrm{PD}$ can be constructed by integrating \[ I_\mathrm{PD}=-\frac{g_\mathrm{A}\pi}{h}\,\Big[\, (1-\lambda)r_\mathrm{P}+\lambda r_\mathrm{D}\,\Big]^2\, \frac{dV}{d\lambda} \] with boundary conditions $V(0)=V_\mathrm{P}$ and $V(1)=V_\mathrm{D}$. This calculation shows that the potentials $V_\mathrm{P}$ and $V_\mathrm{D}$ give rise to axial current \begin{equation}\label{mp2} I_\mathrm{PD}= \frac{\pi g_\mathrm{A} r_\mathrm{P} r_\mathrm{D}}{h}\,\big(\,V_\mathrm{P}-V_\mathrm{D}\,\big) \end{equation} in the absence of distributed and point currents, and that the potential at point $\lambda$ is \begin{equation}\label{mp3} V(\lambda) = \frac{V_\mathrm{P}\,(1-\lambda)\, r_\mathrm{P}+V_\mathrm{D}\,\lambda\,r_\mathrm{D}} {(1-\lambda)\,r_\mathrm{P}+\lambda\,r_\mathrm{D}}\,. \end{equation} Expressions (\ref{mp2}) and (\ref{mp3}) are estimates of the axial current flowing along a segment and the potential distribution within the segment in the absence of transmembrane current. \subsection{Motivation for partitioning point current input - model accuracy}\label{assertion} One inescapable feature of a traditional compartmental model is that small variations in the location of segment boundaries, as might occur when a dendrite is represented by segments, may exert a large influence on the solution of the resulting mathematical model. Consider, for example, a point input close to a segment boundary. A small variation in the position of that boundary may change the assigned location of this input from the centre of one segment to that of an adjacent segment. With respect to the mathematical model, the location of this input is therefore determined only to an accuracy of half a segment length, and this indeterminacy will in turn generate a model solution that is particularly sensitive to segment boundaries -- small changes in these boundaries may lead to large changes in the model solution. Of course, with a small number of point sources of input, this problem can be avoided in a traditional compartmental model by arranging that only one point input falls on a segment, and that the location of this input coincides with the centre of the segment. However, this strategy is not feasible when dealing with large scale point input. What is required is a procedure that describes the effect of point input on a dendritic section in a way that is largely insensitive to how that section is represented by segments. It is essential to recognise that there are two primary sources of error in the construction of a compartmental model; the first is the well-documented effect of discretising a continuous dendrite, and the second pertains to error introduced by the placement of input on this dendrite. In a traditional compartmental model with $n$ compartments, the first type of error is $O(1/n^2)$ (by analogy with the finite difference representation of derivatives), but it is not widely recognised that the second type of error is $O(1/n)$. Since the accuracy of any model must be governed by the least accurate contribution to the model, it is clear that \emph{in practice} a traditional model is $O(1/n)$ accurate. This theoretical observation is supported by the simulation exercises of Subsections \ref{sim1} and \ref{sim2}. By contrast with a traditional compartmental model, the new compartmental model describes the influence of input to an accuracy of $O(1/n^2)$, and therefore one would anticipate that it does not degrade the overall accuracy of the model. This assertion is testable by a simulation exercise. \subsection{Partitioning rule for transmembrane current} In compartmental modelling the effect of input current enters the mathematical model at points, or nodes, at which the membrane potential is known. In a traditional model, these nodes are at the centres of segments, whereas in the new model they are at the boundaries of segments. In the new model, input at any location is partitioned between the nodes at the proximal and distal boundaries of the segment on which the input acts. This procedure ensures that the solution of the mathematical model is insensitive to small changes in the location of segment boundaries simply because changes in these boundaries also affects how the input is partitioned between nodes. In the mathematical model, the effect of input to a segment is treated as perturbations $I_\mathrm{P}$ and $I_\mathrm{D}$ to the axial current $I_\mathrm{PD}$ at the proximal and distal boundaries of a segment. Axial current $I_\mathrm{PD}+I_\mathrm{P}$ is assumed to leave the proximal boundary of a segment in the direction of its distal boundary, while axial current $I_\mathrm{PD}+I_\mathrm{D}$ is assumed to arrive at the distal boundary of a segment from the direction of its proximal boundary. The perturbations $I_\mathrm{P}$ and $I_\mathrm{D}$ must satisfy the conservation of current condition \begin{equation}\label{potc1} (I_\mathrm{PD}+I_\mathrm{D})-(I_\mathrm{PD}+I_\mathrm{P})+h\int_0^1 J(\lambda,t)\,d\lambda=0\quad\rightarrow\quad I_\mathrm{P}-I_\mathrm{D}=h\int_0^1 J(\lambda,t)\,d\lambda \end{equation} where $hJ(\lambda,t)\,d\lambda+o(d\lambda)$ is the transmembrane current crossing the segment in $(\lambda,\lambda+d\lambda)$. The task is to construct expressions for $I_\mathrm{P}$ and $I_\mathrm{D}$ that satisfy (\ref{potc1}) for all constitutive forms for $J(\lambda,t)$. In the new compartmental model, transmembrane current acting at point $\lambda$ is divided between the proximal and distal boundaries of a segment in inverse proportion to the resistance of the segment lying between the point $\lambda$ and that boundary. If $R_\mathrm{P}(\lambda)$ is the axial resistance of the portion of segment lying between the point $\lambda$ and the proximal boundary of the segment, and $R_\mathrm{D}(\lambda)$ is the axial resistance of the portion of segment lying between the point $\lambda$ and the distal boundary of the segment, then \begin{equation}\label{potc2} R_\mathrm{P}(\lambda) = \frac{\lambda h} {\pi g_\mathrm{A} r_\mathrm{P} r(\lambda)}\,,\qquad R_\mathrm{D}(\lambda) = \frac{(1-\lambda)h} {\pi g_\mathrm{A} r_\mathrm{D} r(\lambda)}\,,\qquad R_\mathrm{P}(\lambda)+R_\mathrm{D}(\lambda) = \frac{h} {\pi g_\mathrm{A} r_\mathrm{P} r_\mathrm{D}}\,. \end{equation} The rule for partitioning transmembrane current now leads to the expressions \begin{equation}\label{potc3} I_\mathrm{P} = h\int_0^1 \frac{(1-\lambda)\,r_\mathrm{P}\, J(\lambda,t)\,d\lambda}{(1-\lambda)\,r_\mathrm{P} +\lambda\,r_\mathrm{D}}\,,\qquad -I_\mathrm{D} = h\int_0^1 \frac{\lambda\,r_\mathrm{D}\,J(\lambda,t)\,d\lambda} {(1-\lambda)\,r_\mathrm{P}+\lambda\,r_\mathrm{D}}\,, \end{equation} which clearly satisfy identically condition (\ref{potc1}) for the conservation of current. \subsection{Specification of transmembrane current} Transmembrane current is usually assumed to consist of four distinct components: capacitative current, intrinsic voltage-dependent current, synaptic current and exogenous current. Total transmembrane current is represented by \begin{equation}\label{tc1} \int 2\pi r \,c_\mathrm{M}\,\frac{\partial V}{\partial t}\,dx +\int 2\pi r\,J_\mathrm{IVDC}(V)\,dx+\sum J_\mathrm{SYN}(V_\mathrm{syn}) +\sum I_\mathrm{EX} \end{equation} where the integrals and summations are taken over the length of a segment. In this expression $c_\mathrm{M}$ ($\mu$F/cm$^2$) is the specific capacitance of the segment membrane, $V(x,t)$ is the distribution of membrane potential at time $t$ (msec), $J_\mathrm{IVDC}(V)$ ($\mu$A/cm$^2$) is the density of transmembrane current due to intrinsic voltage-dependent channel activity, $J_\mathrm{SYN}(V_\mathrm{syn})$ ($\mu$A) describes synaptic input and $I_\mathrm{EX}$ ($\mu$A) describes exogenous input. Although the specific capacitance of dendritic membrane is normally taken to be constant in neuronal modelling, it will be treated here as a function of position to show how transmembrane current of this type may be incorporated into the new compartmental model. For a segment of length $h$, the expression for $J(\lambda,t)$ corresponding to formula (\ref{tc1}) is \begin{equation}\label{tc2} \begin{array}{rcl} h J(\lambda,t) & = & \ds 2\pi h r(\lambda)\,c_\mathrm{M}(\lambda)\,\frac{\partial V(\lambda,t)}{\partial t}+2\pi h r(\lambda)\,J_\mathrm{IVDC}(V(\lambda,t))\\[10pt] &&\qquad \ds+\;\sum_k J_\mathrm{SYN}(V_\mathrm{syn})\,\delta(\lambda-\lambda_k) + \sum_k I_\mathrm{EX}(t)\,\delta(\lambda-\lambda_k) \end{array} \end{equation} where $\lambda_k$ denotes the relative location of the $k^{th}$ synapse or exogenous input with respect to the proximal boundary of the segment ($\lambda=0$).