#!/usr/bin/python
from .neuron_builder import NeuronBuilder
from .synapse_builder_ampanmdasynplast import AMPANMDASynPlastBuilder
from neuron import gui
import os
import argparse
import shutil
from datetime import datetime
SynapseType = AMPANMDASynPlastBuilder
class SmartFormatter(argparse.HelpFormatter):
def _split_lines(self, text, width):
if text.startswith('R|'):
return text[2:].splitlines()
return argparse.HelpFormatter._split_lines(self, text, width)
def parse_args():
parser = argparse.ArgumentParser(description='Build and run neuron simulation',
formatter_class=SmartFormatter)
parser.add_argument("-p", "--protocol",
choices=['ltp', 'ltd'],
default="ltp",
help="R|Provide protocol name.\n"
" ltp - 2x bursts 100Hz 1s with 1s interburst interval\n"
" ltd - 1Hz 900s\n"
)
parser.add_argument("-aC", "--aicdchannels", help="How much AICD in channels [0-1]", default="0")
parser.add_argument("-aN", "--aicdnr2b", help="How much AICD for NR2B [0-4]", default="1")
parser.add_argument("-n2b", "--nr2bmult", help="GluN2B multiplier", default="1")
parser.add_argument("-b", "--beta", help="How much Beta Amyloids [0-1]", default="0")
parser.add_argument("-bM", "--beta_midpoint", help="Beta Amyloids LTP hill midpoint multiplier", default="1")
parser.add_argument("-s", "--session", help="Session file", default="main.ses")
args = parser.parse_args()
if args.session != "none" and not os.path.exists(f"sessions/{args.session}"):
print(f"No session file: sessions/{args.session}. Exiting...")
exit(1)
print(f"Protocol: {args.protocol}")
print(f"AICD Channels: {args.aicdchannels}")
print(f"AICD GluN2B: {args.aicdnr2b}")
print("------------------------")
return args
def ltp(args, datestring):
if float(args.aicdchannels) > 0:
hocfile = "cell_seed4_0_AICD_cell.hoc"
else:
hocfile = "cell_seed4_0_control_cell.hoc"
neuron_model = NeuronBuilder(hocfile=hocfile)
synapse = SynapseType()
neuron_parameters = { 'nr2bmult': float(args.nr2bmult)}
synapse_parameters = {}
neuron_model.set_parameters(neuron_parameters)
synapse.set_parameters(synapse_parameters)
neuron_model.set_alzheimers(float(args.aicdchannels), float(args.aicdnr2b))
neuron_model.set_beta_amyloid(float(args.beta), float(args.beta_midpoint))
neuron_model.build_protocol_ltp(synapse)
if args.session != "none":
print(f"Loading session: {args.session}")
neuron_model.load_session(f"sessions/{args.session}")
neuron_model.run()
synapse.print_epsp_change()
def ltd(args, datestring):
if float(args.aicdchannels) > 0:
hocfile = "cell_seed4_0_AICD_cell.hoc"
else:
hocfile = "cell_seed4_0_control_cell.hoc"
neuron_model = NeuronBuilder(hocfile=hocfile)
synapse = SynapseType()
neuron_parameters = { 'nr2bmult': float(args.nr2bmult)}
synapse_parameters = {}
neuron_model.set_parameters(neuron_parameters)
synapse.set_parameters(synapse_parameters)
neuron_model.set_alzheimers(float(args.aicdchannels), float(args.aicdnr2b))
neuron_model.set_beta_amyloid(float(args.beta), float(args.beta_midpoint))
neuron_model.build_protocol_ltd(synapse)
if args.session != "none":
print(f"Loading session: {args.session}")
neuron_model.load_session(f"sessions/{args.session}")
neuron_model.run()
synapse.print_epsp_change()
def main():
global neuron_model, synapse
if 'MILEDIDEBUG' not in os.environ:
os.environ['MILEDIDEBUG'] = '0'
if os.path.isdir('x86_64'):
shutil.rmtree('x86_64')
args = parse_args()
datestring = datetime.now().strftime("%Y.%m.%d.%H.%M.%S")
globals()[args.protocol](args, datestring)
if __name__ == "__main__":
main()