//genesis (R.M. 4/9/2007)
// copied from Golgi cell in granular network
include defaults
str Purkinje_dir = "../Purkinje_cell"
float dt = 2.0e-5
// setting the clocks, this must de done BEFORE creating the hines solver !!!
int i
echo dt = {dt}
for (i=0; i<=7; i=i+1)
setclock {i} {dt}
end
// setclock 1 0.0005
setclock 8 4.0e-5
include ../Purkinje_cell/Purk_const
include ../Purkinje_cell/Purk_chanload // chansave // chanload
include ../Purkinje_cell/Purk_cicomp
//include ../Purkinje_cell/Purk_syn37dC
include ../Purkinje_cell/Purk_syn
if (! {exists /library})
create neutral /library
end
ce /library
// Make the prototypes of channels and compartments that can be invoked in .p files
make_Purkinje_chans
include ../Purkinje_cell/Purk_chanloadNaFshift
make_Purkinje_syns
make_Purkinje_comps
make_Purkinje_spines
if (!{exists /Purkinje_layer})
create neutral /Purkinje_layer
end
str cellpath = "/Purkinje_cell"
// read cell data from .p file
readcell ../Purkinje_cell/psmall.p {cellpath}
// rotate the cell so that PF-axis becomes x-axis
// rotcoord {cellpath} {-3.1415927/2} -z
// There is a bug in rotcoord: compartments and their children are rotated,
// spines are rotated too, but spike heads and par channels are NOT,
// so let us do it brute force (each element separately).
rotcoord /Purkinje_cell/##[CLASS=segment] {-3.1415927/2} -z -fixkids
// add a spikegen object
create spikegen {cellpath}/soma/spike
setfield {cellpath}/soma/spike thresh -0.02 \
abs_refract 0.001 \
output_amp 1
addmsg {cellpath}/soma {cellpath}/soma/spike INPUT Vm
echo making hsolve of Purkinje cells
create hsolve {cellpath}/solve
ce {cellpath}/solve
setfield . path "../##[][TYPE=compartment]" comptmode 1 chanmode 4 // {chanmode}
setfield . computeIm 1
call . SETUP
setmethod 11
reset