//genesis /* This is a graphics file. It is meant for the graphical representation of the activity of a simple compartment containing the HH type delayed rectifier potassium channel and a transient rat brain sodium channel that shows prepulse amplitude and duration dependence. */ //---------------------------- // injection to the cell soma //---------------------------- function inject_soma (dialog) str dialog setfield /input/injectpulse/somacurr gain {getfield {dialog} value} end //=========================== //setting the pulsegen object //=========================== function make_input float injwidth = 0.2 //sec float injdelay = 0.01 //sec float somacurrent = 2e-9 // injection current to soma create neutral /input create pulsegen /input/injectpulse setfield /input/injectpulse level1 1.0 width1 {injwidth} \ delay1 {injdelay} baselevel 0.0 trig_mode 0 delay2 1 create diffamp /input/injectpulse/somacurr setfield /input/injectpulse/somacurr saturation 1 gain {somacurrent} addmsg /input/injectpulse /input/injectpulse/somacurr PLUS output addmsg /input/injectpulse/somacurr /cell/soma INJECT output end //---------------------- // RUN STEP //---------------------- function step_tmax step {tmax} -time end //-------------------- // Overlaying graphs //------------------- function overlaytoggle (widget) str widget setfield /##[TYPE=xgraph] overlay {getfield {widget} state} end //------------------- // The control panel //------------------- function make_control create xform /control [10,10,350,150] create xlabel /control/label -label "Control Panel" \ -hgeom 30 -bg cyan create xbutton /control/RESET -script reset -wgeom 33%\ -ygeom 0:label -offbg yellow create xbutton /control/RUN -script step_tmax -xgeom 0:RESET \ -ygeom 0:label -wgeom 33% -offbg green create xbutton /control/QUIT -script quit -xgeom 0:RUN \ -ygeom 0:label -wgeom 33% -offbg red create xdialog /control/inject -label \ "Current Injection (amperes)" -value 2e-9 \ -script "inject_soma <widget>" create xtoggle /control/overlay \ -script "overlaytoggle <widget>" setfield /control/overlay offlabel "Overlay OFF" onlabel \ "Overlay ON" state 0 offfg blue onfg red xshow /control end //----------------- // GRAPHS //----------------- function pulse_response create xform /data create xform /data/Vm [500,10,400,250] create xlabel /data/Vm/label -label "Voltage Response" \ -hgeom 30 -bg pink create xgraph data/Vm/voltage -xmin -0.01 -xmax 0.2 -ymin -0.10 \ -ymax 0.05 setfield ^ XUnits sec YUnits V addmsg cell/soma /data/Vm/voltage PLOT Vm *soma *red xshow /data/Vm create xform /data/Ik [500,500,400,250] create xlabel /data/Ik/label -label "Current Response" \ -hgeom 30 -bg pink create xgraph data/Ik/current -xmax 0.2 -ymin -2.0e-9 -ymax 5.0e-10 setfield ^ XUnits sec YUnits A addmsg cell/soma/Na_rat_sm /data/Ik/current PLOT Ik *Na *blue addmsg cell/soma/Kdr_hip_traub91 /data/Ik/current PLOT Ik *K *green xshow /data/Ik create xform /data/Gk [10,500,400,250] create xlabel /data/Gk/label -label "Channel Conductances" \ -hgeom 30 -bg pink create xgraph data/Gk/conductances -xmax 0.2 -ymin 0.1 setfield ^ XUnits sec YUnits mho addmsg cell/soma/Na_rat_sm /data/Gk/conductances PLOT Gk *Na *blue addmsg cell/soma/Kdr_hip_traub91 /data/Gk/conductances PLOT Gk *K *green xshow /data/Gk end //******************* // Main Script //******************* make_input make_control pulse_response