# savesynapselocations.py
# A script for randomly picking locations along the apical dendrite (from 300um on)
# A maximum of 50 synapses per segment are allowed, while the total number of synapses
# is 3000.
#
# The input code for the hoc-interface is based on BAC_firing.hoc by Etay Hay (2011)
#
# Tuomo Maki-Marttunen, Jan 2015
# (CC BY)
from neuron import h
import matplotlib
matplotlib.use('Agg')
from pylab import *
import mytools
import pickle
import time
import sys
import random
random.seed(1)
proximalpoint = 400
distalpoint = 620
BACdt = 5.0
fs = 8
maxLens = [1300,1185]
maxSynsPerSeg = 50
Nsyns = 3000
synlocsAll = []
for icell in range(0,2):
morphology_file = "morphologies/cell"+str(icell+1)+".asc"
biophys_file = "models/L5PCbiophys3.hoc"
template_file = "models/L5PCtemplate.hoc"
synlocs = []
h("""
load_file("stdlib.hoc")
load_file("stdrun.hoc")
objref cvode
cvode = new CVode()
cvode.active(1)
load_file("import3d.hoc")
objref L5PC
load_file(\""""+biophys_file+"""\")
load_file(\""""+template_file+"""\")
L5PC = new L5PCtemplate(\""""+morphology_file+"""\")
access L5PC.soma
objref sl,syn1,con1,isyn, tvec, syns["""+str(Nsyns)+"""]
tvec = new Vector()
sl = new List()
double siteVec[2]
""")
synsInSegs = [0]*len(h.L5PC.apic)
for istim in range(0,Nsyns):
myiseg = -1
while myiseg == -1:
x = 300.0+(maxLens[icell]-300)*random.random()
h("""sl = L5PC.locateSites("apic","""+str(x)+""")
Nsegs_x = sl.count()
""")
iseg = random.randint(0,h.Nsegs_x-1)
h("iseg = sl.o["+str(iseg)+"].x[0]")
if synsInSegs[int(h.iseg)] < maxSynsPerSeg:
myiseg = int(h.iseg)
break
print "istim = "+str(istim)+", x = "+str(x)+", continue searching for iseg..."
synsInSegs[myiseg] = synsInSegs[myiseg] + 1
h("""
siteVec[0] = sl.o[j].x[0]
siteVec[1] = sl.o[j].x[1]
L5PC.apic[siteVec[0]] {
syns["""+str(istim)+"""] = new AlphaSynapse(siteVec[1])
syns["""+str(istim)+"""].e = 0
syns["""+str(istim)+"""].tau = 5
syns["""+str(istim)+"""].onset = 10000 + """+str(BACdt)+"""
}
""")
synlocs.append([h.siteVec[0],h.siteVec[1]])
synlocsAll.append(synlocs[:])
picklelist = [Nsyns,maxSynsPerSeg,maxLens,synlocsAll]
file = open('synlocs.sav', 'w')
pickle.dump(picklelist,file)
file.close()