TITLE Store operated calcium channel for bladder small DRG neuron soma model
: Author: Darshan Mandge (darshanmandge@iitb.ac.in)
: Computational Neurophysiology Lab
: Indian Institute of Technology Bombay, India
: For details refer:
: A biophysically detailed computational model of bladder small DRG neuron soma
: Darshan Mandge and Rohit Manchanda, PLOS Computational Biology (2018)
NEURON {
SUFFIX soce
USEION ca READ cai, cao WRITE ica
USEION caer READ caeri VALENCE 2
RANGE minf, mtau
RANGE pmax, ica
THREADSAFE
}
UNITS {
(mA) = (milliamp)
(mV) = (millivolt)
(mM) = (milli/liter)
FARADAY = 96489 (coul)
R = 8.314 (volt-coul/degC)
}
PARAMETER {
v (mV)
celsius (degC)
pmax = 1e-9 (cm/s)
: Luik et al., 2008
kd = 169e-3 (mM) : dissociation constant
nh = 4.2 (1) : hill coefficient
}
STATE {
m
}
ASSIGNED {
ica (mA/cm2)
mtau (ms)
minf (1)
cai (mM)
cao (mM)
caeri (mM)
}
BREAKPOINT {
SOLVE state METHOD cnexp
ica = pmax*m*ghk(v,cai,cao,2)
}
DERIVATIVE state {
rates(caeri)
m'= (minf-m) / mtau
}
INITIAL {
rates(caeri)
m = minf
}
: For GHK Current equation (adapted from Stepheen and Manchanda, 2009)
FUNCTION ghk( v(mV), ci(mM), co(mM), z) (millicoul/cm3) { LOCAL e, w
w = v * (.001) * z*FARADAY / (R*(celsius+273.16))
if (fabs(w)>1e-4)
{ e = w / (exp(w)-1) }
else : denominator is small -> Taylor series
{ e = 1-w/2 }
ghk = - (.001) * z*FARADAY * (co-ci*exp(w)) * e
}
UNITSOFF
PROCEDURE rates(c (mM)) {
UNITSOFF
minf = 1 - (c^nh)/(c^nh+kd^nh) :reverse hill equation : Luik et al., 2008
UNITSON
mtau = 5000 (ms)
}
UNITSON