// pulse.hoc
// add pulse generator Ipulse2 from
// http://www.neuron.yale.edu/neuron/static/docs/repstim/pulsedistrib.zip
objref ip
soma ip=new Ipulse2(0.5)
ip.del=100
ip.dur=10 // duration of pulse
ip.per=240 // period of pulse
ip.num=4
ip.amp=0 // set to .3 when creating figure 7c
proc set_period() {
ip.per=$1
tstop=4*ip.per+2*ip.del
}
xpanel("run pulse generator")
xlabel("Click below to generate traces for")
xbutton("Figure 7c Marsurkar, Chen 2011 (Ca2+)","fig7c()")
xpanel()
objref vbox, g1, g2
objref v_vec, t_vec
proc fig7c() {
vbox=new VBox()
vbox.intercept(1)
g1=new Graph()
set_period(240)
v_vec=new Vector()
t_vec=new Vector()
v_vec.record(&soma.v(0.5))
t_vec.record(&t)
store_iclamp=IClamp[0].amp
IClamp[0].amp=0
ip.amp=.3
init()
run()
v_vec.line(g1, t_vec)
g1.exec_menu("View = plot")
g2=new Graph()
set_period(130)
v_vec=new Vector()
t_vec=new Vector()
v_vec.record(&soma.v(0.5))
t_vec.record(&t)
init()
run()
IClamp[0].amp=store_iclamp
ip.amp=0 // turn off pulses in case want to run IClamp
v_vec.line(g2, t_vec)
g2.exec_menu("View = plot")
vbox.intercept(0)
vbox.map()
}