proc celldef() {
topol()
subsets()
geom()
biophys()
geom_nseg()
diam_seg()
}
create soma, dend1[3], dend1_1[3], dend2[5], SDI, SIprox, SIdistal
create axon
proc topol() { local i
connect dend1(0), soma(0)
for i = 1, 2 connect dend1[i](0), dend1[i-1](1)
for i = 0, 2 connect dend1_1[i](0), soma(1)
for i = 0, 1 connect dend2[i](0), dend1(1)
for i = 2, 3 connect dend2[i](0), dend1[1](1)
connect dend2[4](0), dend1[2](1)
connect SDI(0), dend1[2](1)
connect SIprox(0), SDI(1)
connect SIdistal(0), SIprox(1)
connect axon(0), SIdistal(1)
basic_shape()
}
proc basic_shape() {
soma {pt3dclear() pt3dadd(0, 0, 0, 1) pt3dadd(15, 0, 0, 1)}
dend1 {pt3dclear() pt3dadd(0, 0, 0, 1) pt3dadd(-29, 0, 0, 1)}
dend1[1] {pt3dclear() pt3dadd(-29, 0, 0, 1) pt3dadd(-59, 0, 0, 1)}
dend1[2] {pt3dclear() pt3dadd(-59, 0, 0, 1) pt3dadd(-104, 0, 0, 1)}
dend1_1 {pt3dclear() pt3dadd(15, 0, 0, 1) pt3dadd(90, -59, 0, 1)}
dend1_1[1] {pt3dclear() pt3dadd(15, 0, 0, 1) pt3dadd(120, 0, 0, 1)}
dend1_1[2] {pt3dclear() pt3dadd(15, 0, 0, 1) pt3dadd(105, 60, 0, 1)}
dend2 {pt3dclear() pt3dadd(-29, 0, 0, 1) pt3dadd(-29, 60, 0, 1)}
dend2[1] {pt3dclear() pt3dadd(-29, 0, 0, 1) pt3dadd(-29, -59, 0, 1)}
dend2[2] {pt3dclear() pt3dadd(-59, 0, 0, 1) pt3dadd(-59, 60, 0, 1)}
dend2[3] {pt3dclear() pt3dadd(-59, 0, 0, 1) pt3dadd(-59, -59, 0, 1)}
SDI {pt3dclear() pt3dadd(-104, 0, 0, 1) pt3dadd(-119, 15, 0, 1)}
SIprox {pt3dclear() pt3dadd(-119, 15, 0, 1) pt3dadd(-134, 30, 0, 1)}
SIdistal {pt3dclear() pt3dadd(-134, 30, 0, 1) pt3dadd(-164, 60, 0, 1)}
axon {pt3dclear() pt3dadd(-164, 60, 0, 1) pt3dadd(-194, 165, 0, 1)}
dend2[4] {pt3dclear() pt3dadd(-104, 0, 0, 1) pt3dadd(-164, -44, 0, 1)}
}
objref all, Secdend
proc subsets() { local i
objref all, Secdend
all = new SectionList()
Secdend = new SectionList()
soma all.append()
for i=0, 2 dend1[i] all.append()
for i=0, 2 dend1_1[i] all.append()
for i=0, 4 dend2[i] all.append()
SDI all.append()
SIprox all.append()
SIdistal all.append()
axon all.append()
for i=0, 4 dend2[i] Secdend.append()
}
proc geom() {
forsec all { }
soma.L = 20
dend1.L = 27
dend1[1].L = 26
dend1[2].L = 27
dend1_1.L = 500
dend1_1[1].L = 500
dend1_1[2].L = 500
dend2.L =500
dend2[1].L = 500
dend2[2].L = 500
dend2[3].L =500
dend2[4].L = 500 //axonal
SDI.L = 21
SIprox.L = 15
SIdistal.L = 15
axon.L = 800
}
proc geom_nseg() {
forsec all { nseg = int((L/(0.1*lambda_f(1000))+.999)/2)*2 + 1 }
}
proc diam_seg() {
soma.diam = 20
dend1.diam = 3.3
dend1[1].diam = 2.75
dend1[2].diam = 2.4
dend1_1.diam(0:1) = 3:0.5
dend1_1[1].diam(0:1) = 3:0.5
dend1_1[2].diam(0:1) = 3:0.5
dend2.diam(0:1) = 2 : 0.5
dend2[1].diam(0:1) = 2 : 0.5
dend2[2].diam(0:1) = 2 : 0.5
dend2[3].diam(0:1) = 2 : 0.5
dend2[4].diam(0:1) = 2:0.5
SDI.diam = 1.5
SIprox.diam = 1.15
SIdistal.diam = 1
axon.diam = 0.7
}
proc biophys() {
forsec all {
Ra = 150
cm = 0.75
}
hhN=75 // for Ina and *2 for kdrDA (homo)
CaN = 1// for Ica and *x for kca (homo)
KCA= 0.1
hhsec = 75 // for Ina and *2 for kdrDA (secondary dendrites : hetero)
Casec =1// for Ica and *x (secondary dendrites : hetero)
KCAsec= 0.1
hhABD =75 // for Ina and *2 for kdrDA (ABD : hetero)
CaABD = 1// for Ica and *x (ABD: hetero)
KCAABD =0.1
soma {
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kaDasoma
gbar_kaDasoma = 150
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaN
insert cad
insert kca
gbar_kca = KCA
ena=60
ek=-90
}
dend1 {
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend1[1] {
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend1[2] {
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend1_1 {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend1_1[1] {
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend1_1[2] {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kdrDA
gbar_kdrDA = hhN*2
insert Na12
gbar_Na12 = hhN
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend2 {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend2[1] {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kdrDA
gbar_kdrDA = hhN*2
insert Na12
gbar_Na12 = hhN
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend2[2] {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend2[3] {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert kdrDA
gbar_kdrDA = hhN*2
insert Na12
gbar_Na12 = hhN
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
dend2[4] {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
SDI {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert Na12
gbar_Na12 = hhN
insert kdrDA
gbar_kdrDA = hhN*2
insert kaDa
gbar_kaDa = 100
insert Ih
gbar_Ih = 3
insert CAV13
gbar_CAV13 = CaABD
insert cad
insert kca
gbar_kca = KCAABD
ena=60
ek=-90
}
SIprox {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert Na12
gbar_Na12 = 2000
insert kdrDA
gbar_kdrDA = 1000
ena=60
ek=-90
}
SIdistal {
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
insert Na12
gbar_Na12 = 2000
insert kdrDA
gbar_kdrDA =1000
ena=60
ek=-90
}
axon {
insert Na12
gbar_Na12 = 400
insert kdrDA
gbar_kdrDA = 400
insert pasnts
g_pasnts = 1e-05
e_pasnts = -50
ena=60
ek=-90
}
}