/*----------------------------------------------------------------------------
demo file
---------
Simulations of networks using the object-oriented ability of NEURON
2 neurons reciprocally connected with GABA-B synapses
+ self-connections
Idem Fig.13B of the J Neurophysiol. paper
Reference:
Destexhe, A., Contreras, D., Sejnowski, T.J. and Steriade, M. A model of
spindle rhythmicity in the isolated reticular thalamus. Journal of
Neurophysiology 72: 803-818, 1994.
See also:
http://www.cnl.salk.edu/~alain
http://cns.fmed.ulaval.ca
----------------------------------------------------------------------------*/
//----------------------------------------------------------------------------
// load and define general graphical procedures
//----------------------------------------------------------------------------
//xopen("$(NEURONHOME)/lib/hoc/stdrun.hoc")
objectvar g[20] // max 20 graphs
ngraph = 0
proc addgraph() { local ii // define subroutine to add a new graph
// addgraph("variable", minvalue, maxvalue)
ngraph = ngraph+1
ii = ngraph-1
g[ii] = new Graph()
g[ii].size(0,tstop,$2,$3)
g[ii].xaxis()
g[ii].yaxis()
g[ii].addvar($s1,1,0)
g[ii].save_name("graphList[0].")
graphList[0].append(g[ii])
}
if(ismenu==0) {
nrnmainmenu() // create main menu
nrncontrolmenu() // create control menu
ismenu=1
}
//----------------------------------------------------------------------------
// general parameters
//----------------------------------------------------------------------------
dt=0.1
tstop = 7000
runStopAt = tstop
steps_per_ms = 5
celsius = 36
v_init = -70
//----------------------------------------------------------------------------
// create cells
//----------------------------------------------------------------------------
load_file("RE.tem") // load template for RE cell
ncells = 2 // sets the number of cells
objectvar RE[ncells] // create an array of object variables
for(i=0; i<ncells; i=i+1) {
RE[i] = new REcell() // create RE cells from template
}
//----------------------------------------------------------------------------
// create connections
//----------------------------------------------------------------------------
neigh = 2 // number of neighbors connected
// neigh=1 interconnections
// neigh=2 idem + self synapse
objectvar syn[ncells][ncells] // create object variables for synapses
for(i=0; i<ncells; i=i+1) { // scan over each pair of cells
nv = 0
for(j=0; j<ncells; j=j+1) {
if( (i==j) && (neigh==1) ) {
print "<< no self synapse in cell ",i," >>"
} else {
print "<< GABAB1 synapse number ",nv," from cell ",i," to cell ",j," >>"
syn[i][nv] = new GABAB1()
// postsynaptic is soma of RE[j]
RE[j].soma syn[i][nv].loc(0.5)
// presynaptic is soma of RE[i]
setpointer syn[i][nv].pre, RE[i].soma.v(0.5)
nv = nv + 1
}
}
}
print " "
//----------------------------------------------------------------------------
// create procedure for setting the synaptic weights
//----------------------------------------------------------------------------
proc assign_synapses() { // assign a value to all synapses
for(i=0; i<ncells; i=i+1) { // scan over each cell
for(j=0; j<neigh; j=j+1) { // scan over each connection
syn[i][j].gmax = $1 // assign value of gmax
}
}
}
assign_synapses(0.005) // assign value (in uS) to each synapse
// sum of all synapses must be of 0.01uS
// for each cell
//----------------------------------------------------------------------------
// insert random current pulse in each neuron
//----------------------------------------------------------------------------
objectvar RG,Pulse[ncells] // create object variables
RG = new Random() // create random generator
for (i=0; i<ncells; i=i+1) {
print "defining current pulse in RE[",i,"]"
RE[i].soma Pulse[i] = new IClamp(.5) // create current stim
// note: for older versions of NEURON, please use PulseStim instead of IClamp
Pulse[i].dur = 200 // fixed duration
Pulse[i].amp = RG.uniform(0,-0.05) // random amplitude
Pulse[i].del = RG.uniform(0,2000) // random latency
}
//----------------------------------------------------------------------------
// add graphs
//----------------------------------------------------------------------------
print " "
print " << Creating graphics ... >>"
print " "
for(i=0; i<ncells; i=i+1) {
addgraph("RE[i].soma.v(0.5)",-100,20)
}