NEURON TUTORIAL FOR IMPLEMENTING SIMULATIONS OF NETWORKS OF THALAMIC RETICULAR NEURONS Alain Destexhe Laboratory for Computational Neuroscience Unité de Neurosciences Intégratives et Computationnelles (UNIC) Centre National de la Recherche Scientifique (CNRS) 91198 Gif-sur-Yvette, France Formerly from (when this work was created): Department of Physiology, Laval University, Quebec G1K 7P4, Canada Destexhe@iaf.cnrs-gif.fr http://cns.iaf.cnrs-gif.fr/Main.html This package is running with the NEURON simulation program written by Michael Hines and available on internet at: http://www.neuron.yale.edu/neuron/ http://neuron.duke.edu/ The package contains mechanisms (.mod files) and programs (.oc files) needed to simulate networks of thalamic reticular (RE) neurons interacting with inhibitory synapses. The approach followed here uses the object-oriented facilities of NEURON to simulate networks, and can be applied to other types of neurons and/or other types of synaptic interactions. This model studies the genesis of 10Hz oscillations by inhibitory networks of RE neurons in the thalamus. Each neuron is described by a single-compartment containing voltage- and calcium-dependent currents described by Hodgkin-Huxley type of model. Synaptic interactions are handled by kinetic models of postsynaptic receptors of the GABA_A and GABA_B types. The models given here are described in detail in the following paper: Destexhe, A., Contreras, D., Sejnowski, T.J. and Steriade, M. A model of spindle rhythmicity in the isolated thalamic reticular nucleus. Journal of Neurophysiology, 72: 803-818, 1994. A postscript version of this paper, including figures, is available on internet at http://cns.iaf.cnrs-gif.fr/Main.html PROGRAMS ======== Each program reproduces one figure of the paper: Fig5A.hoc : 2 neurons connected with GABA_A synapses Fig5B.hoc : 2 neurons connected with GABA_A synapses + self-connections Fig6A.hoc : 5 neurons connected with GABA_A synapses (first-neighbor) Fig6B.hoc : 5 neurons connected with GABA_A synapses (first-neighbor+self) Fig6C.hoc : 5 neurons connected with GABA_A synapses (2nd-neighbor) Fig6D.hoc : 5 neurons connected with GABA_A synapses (all-to-all) Fig13A.hoc : 2 neurons connected with GABA_B synapses Fig13B.hoc : 2 neurons connected with GABA_B synapses + self-connections MECHANISMS ========== HH2.mod : Hodgkin-Huxley kinetics for action potentials IT2.mod : low-threshold calcium current of thalamic RE neurons IAHP.mod : calcium-activated K+ current responsible for AHP ICAN.mod : calcium-activated nonspecific cation current capump.mod : calcium clearance mechanisms gaba.mod : two-state kinetic model for GABA_A receptors gabab1.mod : two-state kinetic model for GABA_B receptors HOW TO RUN ========== Use autolaunch on modeldb or: unix platform: To compile the demo, NEURON and INTERVIEWS must be installed and working on the machine you are using. Just type "nrnivmodl" to compile the mechanisms given in the mod files. Then, execute the main demo program by typing: nrngui mosinit.hoc mswin platform: Compile the mechanism (mod) files by using mknrndll. Then start the simulation by clicking on mosinit.hoc in windows explorer. back to any platform: Once the menu and graphics interface has appeared, click on a simulation from the Simulations of thalamic reticular network window. Reposition the windows to find the Run Control window. Then click on "Init and Run" button to start the simulation on the RunControl window. For more information about how to get NEURON and how to install it, please refer to the following sites: http://www.neuron.yale.edu/neuron/ http://neuron.duke.edu/ For further information, please contact: Alain Destexhe Laboratory for Computational Neuroscience Unité de Neurosciences Intégratives et Computationnelles (UNIC) Centre National de la Recherche Scientifique (CNRS) 91198 Gif-sur-Yvette, France Destexhe@iaf.cnrs-gif.fr http://cns.iaf.cnrs-gif.fr/Main.html 7/11/2005 this model updated to close windows and reopen a run control window between simulations. ModelDB-Note: This model updated for NEURON version 4.3.1 and higher. 10-2007: AD, better synaptic mechanisms using counters 20120106 changed mod files for better solve methods (avoid euler) as per web page http://www.neuron.yale.edu/phpbb/viewtopic.php?f=28&t=592