# NEURON Code for Implementing 2D Olfactory Bulb (OB) Model of Gamma Oscillations
**Author:**
Guoshi Li
Department of Psychiatry
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
**Reference:**
Li G and Cleland TA (2017). A coupled-oscillator model of olfactory bulb gamma oscillations. *PLoS computational biology*, 13(11), e1005760.
For questions, please email: [guoshi_li@med.unc.edu](mailto:guoshi_li@med.unc.edu)
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## Overview
The OB model is implemented with **NEURON 7.3**, and simulations are run under both **CentOS Linux** and **Ubuntu Linux**.
The default OB network model contains:
- 25 mitral cells (MCs)
- 25 periglomerular cells (PGs)
- 100 granule cells (GCs)
### Package Contents
The package contains the following folders:
- **`celldata`**: Stores data from single-cell simulations
- **`data0`**: Stores data from network simulations
- **`connection`**: Stores connectivity information between MCs and GCs
- **`Input`**: Stores odor input values
- **`Readme`**: Contains model information
### Running Simulations
- **Network simulation**: Run `mosinit.hoc`
- **MC single-cell simulation**: Run `MC_Stim.hoc`
- **GC single-cell simulation**: Run `GC_Stim.hoc`
- **PG single-cell simulation**: Run `PG_Stim.hoc`
### Major HOC Files
- **`Parameter.hoc`**: Specifies the parameters of the OB model
- **`Connect.hoc`**: Specifies network connectivity
- **`Background.hoc`**: Generates random background inputs to the network
- **`Input.hoc`**: Generates odor inputs to the network
- **`Figure.hoc`**: Generates graphic displays of network simulation results (optional)
- **`SaveData.hoc`**: Saves all relevant data into files for later analysis
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## Simulation Details
- **Simulation step**: 0.002 ms
- **Default simulation time**: 3000 ms (3 seconds)
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## Data Analysis
The data saved in the `data0` folder after simulation can be analyzed using the following custom MATLAB scripts.
**Note**: The simulation time must be 3000 ms (3 seconds) for the MATLAB scripts to run properly.
- **`PlotV.m`**: Plots cell membrane voltages
- **`Pro_Delay.m`**: Plots spike propagation delay of one representative MC
- **`PlotG.m`**: Plots GABAa conductances
- **`Rasterplot.m`**: Generates raster plots of spikes
- **`LFP.m`**: Performs frequency analysis of the simulated local field potential (sLFP)
- **`Load_data.m`**: Loads all relevant data into the workspace (to save the data into a `.mat` file for future analysis)
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## Changelog
- **2022-05**: Updated MOD files to compile with the latest NEURON releases where ion variables used as `STATE` cannot be declared as `GLOBAL`.
- **2025-04-06**: Reformatted readme. Removed backup files and `__MACOSX` folder. Added full reference information (was previously listed as "in press")