: Chapman JB, Johnson EA, Kootsey JM. (1983)
: Electrical and Biochemical Properties of an Enzyme Model of the Sodium Pump
: J. Membrane Biol. 74, 139-153
: note default step 5 voltage dependence
: extended by Michael Hines as a component of larger models.
: I.e. modifies nai, ki, contributes to ina, ik, and consumes atp
: for investigation of isolated pump, allow clamping of
: nai, ki, atp (note p and adp are constant here)
: initialize to steady state pump with nai, ki, atp clamped.
NEURON {
SUFFIX nakpump
USEION na READ nai WRITE ina
USEION k READ ko WRITE ik
RANGE ik, ina
RANGE nain, naout, kin, kout, smalla, b1, b2, celsiusT, kvotqt
}
UNITS {
(molar) = (1/liter) : moles do not appear in units
(mM) = (millimolar)
(uA) = (microamp)
(mA) = (milliamp)
(mV) = (millivolt)
(um) = (micron)
}
PARAMETER {
: nain = 63.4 (mmol/l)
: kout = 5.6 (mmol/l)
nain = 11.4 (mM)
kout = 5.6 (mM)
smalla = 0 (mA/cm2)
b1 = 1 (mM)
: b1 = 0.5 (mM) :testing for disp
:b2 = 30 (mM)
kvotqt
celsiusT = 32
}
ASSIGNED {
ina (mA/cm2)
ik (mA/cm2)
nai (mM)
ko (mM)
}
STATE {
inapump (mA/cm2)
ikpump (mA/cm2)
}
INITIAL {
inapump = 0
ikpump = 0
}
BREAKPOINT {
: kvotqt = 2.1^((celsiusT-22)/10)
kvotqt = 1^((celsius-22)/10)
ikpump = smalla/((1+b1/ko)^2) * (1.62/(1+(6.7(mM)/(nai+8(mM)))^3) + 1.0/(1+(67.6(mM)/(nai+8(mM)))^3))
ikpump = ikpump*kvotqt
inapump = -1.5*ikpump
ina = inapump
ik = ikpump
}