: Author: David Catherall; Grill Lab; Duke University
: Created: November 2016
: Ka is the Early Transient Outward K current in Schild 1994
: Neuron Block creates mechanism
NEURON {
SUFFIX ka :Sets suffix of mechanism for insertion into models
USEION k READ ek WRITE ik :Lays out which NEURON variables will be used/modified by file
RANGE gbar, ek, ik, shiftka :Allows variables to be modified in hoc and collected in vectors
}
: Defines Units different from NEURON base units
UNITS {
(S) = (siemens)
(mV) = (millivolts)
(mA) = (milliamp)
}
: Defines variables which will have a constant value throughout any given simulation run
PARAMETER {
gbar =0.000141471 (S/cm2) : (S/cm2) Channel Conductance
Q10ka=1.93 :All gating variables have the same constant
Q10TempA = 22.85 (degC) : Used to shift tau values based on temperature with equation : tau(T1)=tau(Q10TempA)*Q10^((Q10TempA-T1)/Q10TempB)
Q10TempB = 10 (degC)
shiftka=3.0 (mV) : Shift factor present in C-fiber
:ka_p Variables
: Steady State Variables
V0p5p=-28.0 (mV):As defined by Schild 1994, zinf=1.0/(1.0+exp((V-V0p5z)/(-S0p5z))
S0p5p=28.0 (mV)
: Tau Variables
A_taup=5.0 (ms) :As defined by Schild 1994, tauz=A_tauz*exp(-B^2(V-Vpz)^2)+C
B_taup=0.022 (/mV)
C_taup=2.5 (ms)
Vpp=-65.0 (mV)
:ka_q Variables
: Steady State Variables
V0p5q=-58.0 (mV)
S0p5q=-7.0 (mV)
: Tau Variables
A_tauq=100.0 (ms)
B_tauq=0.035 (/mV)
C_tauq=10.5 (ms)
Vpq=-30.0 (mV)
}
: Defines variables which will be used or calculated throughout the simulation which may not be constant. Also included NEURON provided variables, like v, celsius, and ina
ASSIGNED {
:NEURON provided Variables
v (mV) : NEURON provides this
ik (mA/cm2)
celsius (degC)
ek (mV)
:Model Specific Variables
g (S/cm2)
tau_q (ms)
tau_p (ms)
pinf
qinf
}
: Defines state variables which will be calculated by numerical integration
STATE { p q }
: This block iterates the state variable calculations and uses those calculations to calculate currents
BREAKPOINT {
SOLVE states METHOD cnexp
g = gbar * p^3 * q
ik = g * (v-ek)
}
: Intializes State Variables
INITIAL {
rates(v) : set tau_m, tau_h, hinf, minf
: assume that equilibrium has been reached
p = pinf
q = qinf
}
:Defines Governing Equations for State Variables
DERIVATIVE states {
rates(v)
p' = (pinf - p)/tau_p
q' = (qinf - q)/tau_q
}
: Any other functions go here
:rates is a function which calculates the current values for tau and steady state equations based on voltage.
FUNCTION rates(Vm (mV)) (/ms) {
tau_p = A_taup*exp(-(B_taup)^2*(Vm-Vpp)^2)+C_taup
pinf = 1.0/(1.0+exp((Vm-V0p5p+shiftka)/(-S0p5p)))
tau_q = A_tauq*exp(-(B_tauq)^2*(Vm-Vpq)^2)+C_tauq
qinf = 1.0/(1.0+exp((Vm-V0p5q+shiftka)/(-S0p5q)))
:This scales the tau values based on temperature
tau_p=tau_p*Q10ka^((Q10TempA-celsius)/Q10TempB)
tau_q=tau_q*Q10ka^((Q10TempA-celsius)/Q10TempB)
}